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Rb X (HIP1R)

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货号：AB9882

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, R, M WB, ICC Rb Purified Polyclonal Antibody
Description
Catalogue Number AB9882
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Huntingtin-Interacting Protein 1 Related (HIP1R) Antibody
Alternate Names
  • HIP1R
Background Information Huntingtin-Interacting protein-1 related (HIP1R) is an actin-binding protein that is considered an element of the clathrin-coated pits and vesicles and polymerizes actin. HIP1R is essential for normal actin organization and endocytosis. HIP1R is equipped with a epsin N-terminal homology domain which allows it to bind 3-phosphorylated inositol lipids, which allows HIP1R to function as a tyrosine kinase stabilizing receptor and plays a role in overall cell growth and survival.
References
Product Information
Format Purified
Presentation Liquid
Applications
Application Detect Huntingtin-Interacting Protein 1 Related (HIP1R) using this Anti-Huntingtin-Interacting Protein 1 Related (HIP1R) Antibody validated for use in WB & IC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application Notes Western Blot: a 1:500-1:2,000 dilution of this antibody detected Huntingtin-interacting protein 1 related (HIP1R) in 10 µg of rat brain extract and mouse brain post-nuclear supernatant.A 1:500-1:2,000 dilution of this antibody detected Huntingtin-interacting protein 1 related (HIP1R) in 10 µg of rat brain extract and mouse brain post-nuclear supernatant.

Immunocytochemistry: a previous lot of this antibody was used by an independent laboratory for IC of human cells. (Poupon, V., et al. (2008). PNAS. 105(1): 168-173.)

Optimal working dilutions must be determined by end user.
Biological Information
Immunogen Synthetic peptide
Host Rabbit
Specificity Huntingtin-interacting protein 1 related (HIP1R). By Western blot the antibody recognizes a band at ~116 kDa on P2 microsomal fraction from embryonic rat brain.
Species Reactivity
  • Human
  • Rat
  • Mouse
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIP1R
  • FLJ14000
  • HIP3
  • ILWEQ
  • HIP12
  • Hip1-related
  • MGC47513
  • KIAA0655
UniProt Number
UniProt Summary FUNCTION: SwissProt: O75146 # Component of clathrin-coated pits and vesicles, that may link the endocytic machinery to the actin cytoskeleton.
SIZE: 1068 amino acids; 119388 Da
SUBUNIT: Interacts with actin and huntingtin interacting protein 1 (HIP1). Does not interact with huntingtin (By similarity).
SUBCELLULAR LOCATION: Cytoplasm, perinuclear region (By similarity). Intracytoplasmic membrane (By similarity). Note=Membrane-associated protein, mainly localized at the endocytic compartments and in the perinuclear region (By similarity).
TISSUE SPECIFICITY: Brain, heart, kidney, pancreas, and liver, but not in lung or placenta.
DOMAIN: SwissProt: O75146 The talin-like domain binds F-actin (By similarity).
SIMILARITY: Belongs to the SLA2 family. & Contains 1 ENTH (epsin N-terminal homology) domain. & Contains 1 I/LWEQ domain.
Molecular Weight ~116 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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