Anti-PLC g-1 (mixed monoclonal antibodies)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R, Rb, B	ICC, IP, WB	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R, Rb, B

ICC, IP, WB

Purified

Monoclonal Antibody

Description
Catalogue Number	05-163
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-PLCγ-1 Antibody
Overview	An equal mix of IgG antibodies produced by SP2/0-Ag14 x Balb/c spleen cells1 and propagated as ascites. Clones B-2-5, B-6-4, B-20-3, D-7-3, E-9-4.
Alternate Names	1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1 Phosphoinositide phospholipase C Phospholipase C-gamma-1 monophosphatidylinositol phosphodiesterase phosphatidylinositol phospholipase C phosphoinositidase C phospholipase C gamma 1 phospholipase C, gamma 1 phospholipase C, gamma 1 (formerly subtype 148) phospholipase C-148 triphosphoinositide phosphodiesterase
Background Information	Phospholipase C gamma 1 is a tyrosine kinase substrate for many receptor and nonreceptor tyrosine kinases. Activation of the enzyme produces two second messenger molecules, inositol 1,4,5-trisphosphate and diacylglycerol, which provoke the mobilization of intracellular Ca2+ and activation of protein kinase C. Although the mammalian genome encodes 10 known phosphoinositide specific PLCs, only the gamma 1 and gamma 2 isoforms are regulated by tyrosine kinase activity. PLC beta isoform activity is controlled by heterotrimeric G protein coupled receptors, while the mechanism of regulation of PLCgamma activity is unknown.

Description

Catalogue Number

05-163

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-PLCγ-1 Antibody

Overview

An equal mix of IgG antibodies produced by SP2/0-Ag14 x Balb/c spleen cells1 and propagated as ascites. Clones B-2-5, B-6-4, B-20-3, D-7-3, E-9-4.

Alternate Names

1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1
Phosphoinositide phospholipase C
Phospholipase C-gamma-1
monophosphatidylinositol phosphodiesterase
phosphatidylinositol phospholipase C
phosphoinositidase C
phospholipase C gamma 1
phospholipase C, gamma 1
phospholipase C, gamma 1 (formerly subtype 148)
phospholipase C-148
triphosphoinositide phosphodiesterase

Background Information

Phospholipase C gamma 1 is a tyrosine kinase substrate for many receptor and nonreceptor tyrosine kinases. Activation of the enzyme produces two second messenger molecules, inositol 1,4,5-trisphosphate and diacylglycerol, which provoke the mobilization of intracellular Ca2+ and activation of protein kinase C. Although the mammalian genome encodes 10 known phosphoinositide specific PLCs, only the gamma 1 and gamma 2 isoforms are regulated by tyrosine kinase activity. PLC beta isoform activity is controlled by heterotrimeric G protein coupled receptors, while the mechanism of regulation of PLCgamma activity is unknown.

Product Information
Format	Purified
Control	A431 cell lysate, HeLa cell lysate Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.
Presentation	Liquid in 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl and 0.05% sodium azide. An equal mix of IgG antibodies produced by SP2/0-Ag14 x Balb/c spleen cells (Suh, P.G., 1988) and propagated as ascites. Clones B-2-5, B-6-4, B-20-3, D-7-3, E-9-4.

Product Information

Format

Purified

Control

A431 cell lysate, HeLa cell lysate
Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.

Presentation

Liquid in 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl and 0.05% sodium azide.
An equal mix of IgG antibodies produced by SP2/0-Ag14 x Balb/c spleen cells (Suh, P.G., 1988) and propagated as ascites. Clones B-2-5, B-6-4, B-20-3, D-7-3, E-9-4.

Applications
Application	Anti-PLCγ-1 Antibody detects level of PLCγ-1 & has been published & validated for use in IC, IP & WB.
Key Applications	Immunocytochemistry Immunoprecipitation Western Blotting
Application Notes	Immunoprecipitation: 4 µg of a previous lot of antibody immunoprecipitated PLCγ-1 from non-stimulated A431 cell lysate, previous lots immunoprecipitated PLCγ-1 from rabbit brain cytosol preparation. Immunocytochemistry: 5-10 µg/mL of previous lots of this antibody detected PLCγ-1 on ethanol-acetic acid [95:5] fixed A431 cells.

Applications

Application

Anti-PLCγ-1 Antibody detects level of PLCγ-1 & has been published & validated for use in IC, IP & WB.

Key Applications

Immunocytochemistry
Immunoprecipitation
Western Blotting

Application Notes

Immunoprecipitation:
4 µg of a previous lot of antibody immunoprecipitated PLCγ-1 from non-stimulated A431 cell lysate, previous lots immunoprecipitated PLCγ-1 from rabbit brain cytosol preparation.

Immunocytochemistry:
5-10 µg/mL of previous lots of this antibody detected PLCγ-1 on ethanol-acetic acid [95:5] fixed A431 cells.

Biological Information
Immunogen	Five individual clones raised against purified soluble phospholipase C (PLC)γ-1 from bovine brain.
Clone	Clonal blend B-2-5, B-6-4, B-20-3, D-7-3, E-9-4.
Host	Mouse
Specificity	This antibody recognizes PLCγ-1, Mr 135 kDa. Reacts with some non-PLCγ-1 SH-3 containing proteins, including nck, Mr 47kDa (Park, D., 1992).
Isotype	mixed IgGs
Species Reactivity	Human Mouse Rat Rabbit Bovine
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_002660.2
Entrez Gene Summary	The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.
Gene Symbol	PLCG1 PLC1 PLCgamma1 PLC148 PLC-II PLC-148 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate PLC-gamma-1 inositoltrisphosphohydrolase
Purification Method	Protein G Chromatography
UniProt Number	P19174
UniProt Summary	FUNCTION: SwissProt: P19174 # PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. COFACTOR: Calcium. SIZE: 1290 amino acids; 148532 Da SUBUNIT: Interacts with CENTG1 via its SH3 domain (By similarity). Interacts with phosphorylated LAT upon TCR activation. Interacts with the Pro-rich domain of TNK1 via its SH3 domain. Associates with BLNK, VAV1, GRB2 and NCK1 in a B-cell antigen receptor- dependent fashion. Interacts with CBLB in activated T-cells; which inhibits phosphorylation. Interacts with SHB. Interacts via its SH3 domain with the Arg/Gly-rich-flanked Pro-rich domains of KHDRBS1/SAM68. This interaction is selectively regulated by arginine methylation of KHDRBS1/SAM68. Interacts with INPP5D/SHIP1 (By similarity). PTM: The receptor-mediated activation of PLC-gamma-1 and PLC- gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. & Ubiquitinated by CBLB in activated T-cells (By similarity). SIMILARITY: SwissProt: P19174 ## Contains 1 C2 domain. & Contains 1 EF-hand domain. & Contains 2 PH domains. & Contains 1 PI-PLC X-box domain. & Contains 1 PI-PLC Y-box domain. & Contains 2 SH2 domains. & Contains 1 SH3 domain. ...
Molecular Weight	135 kDa

Biological Information

Immunogen

Five individual clones raised against purified soluble phospholipase C (PLC)γ-1 from bovine brain.

Clone

Clonal blend B-2-5, B-6-4, B-20-3, D-7-3, E-9-4.

Host

Mouse

Specificity

This antibody recognizes PLCγ-1, Mr 135 kDa. Reacts with some non-PLCγ-1 SH-3 containing proteins, including nck, Mr 47kDa (Park, D., 1992).

Isotype

mixed IgGs

Species Reactivity

Human
Mouse
Rat
Rabbit
Bovine

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_002660.2

Entrez Gene Summary

The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.

Gene Symbol

PLCG1
PLC1
PLCgamma1
PLC148
PLC-II
PLC-148
1-phosphatidyl-D-myo-inositol-4,5-bisphosphate
PLC-gamma-1
inositoltrisphosphohydrolase

Purification Method

Protein G Chromatography

UniProt Number

P19174

UniProt Summary

FUNCTION: SwissProt: P19174 # PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
COFACTOR: Calcium.
SIZE: 1290 amino acids; 148532 Da
SUBUNIT: Interacts with CENTG1 via its SH3 domain (By similarity). Interacts with phosphorylated LAT upon TCR activation. Interacts with the Pro-rich domain of TNK1 via its SH3 domain. Associates with BLNK, VAV1, GRB2 and NCK1 in a B-cell antigen receptor- dependent fashion. Interacts with CBLB in activated T-cells; which inhibits phosphorylation. Interacts with SHB. Interacts via its SH3 domain with the Arg/Gly-rich-flanked Pro-rich domains of KHDRBS1/SAM68. This interaction is selectively regulated by arginine methylation of KHDRBS1/SAM68. Interacts with INPP5D/SHIP1 (By similarity).
PTM: The receptor-mediated activation of PLC-gamma-1 and PLC- gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. & Ubiquitinated by CBLB in activated T-cells (By similarity).
SIMILARITY: SwissProt: P19174 ## Contains 1 C2 domain. & Contains 1 EF-hand domain. & Contains 2 PH domains. & Contains 1 PI-PLC X-box domain. & Contains 1 PI-PLC Y-box domain. & Contains 2 SH2 domains. & Contains 1 SH3 domain. ...

Molecular Weight

135 kDa

Product Usage Statements
Quality Assurance	Routinely evaluated in human A431 carcinoma cells, rabbit brain cytosol, and bovine brain cytosol preparations. Western Blot Analysis: 0.5-2 µg/mL of this lot detected PLCγ-1 in human A431 carcinoma cells; previous lots detected PKCγ-1 in rabbit brain cytosol and bovine brain cytosol preparations.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated in human A431 carcinoma cells, rabbit brain cytosol, and bovine brain cytosol preparations.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected PLCγ-1 in human A431 carcinoma cells; previous lots detected PKCγ-1 in rabbit brain cytosol and bovine brain cytosol preparations.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20ºC from date of shipment.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20ºC from date of shipment.

Packaging Information
Material Size	50 µg

Packaging Information

Material Size

50 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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Anti-PLC g-1 (mixed monoclonal antibodies)

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