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Qiagen 凯杰 Biosharp Omega

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A CALCINEURIN/PP2B B 50UG

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货号：05-187

品牌：Millipore 密理博

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Replacement Information

特卖

100% Performance Guaranteed

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, R WB, IHC M Purified Monoclonal Antibody
Description
Catalogue Number 05-1587
Replaces 04-1007
Description Anti-FOXO3a/FKHRL1 Antibody, clone 15F7.2
Alternate Names
  • Forkhead box protein O3
  • AF6q21 protein
  • Forkhead in rhabdomyosarcoma-like 1
Background Information Forkhead box protein O3 (FOXO3a), or FKHRL1, is a ubiquitous transcription factor that triggers the expression of pro-apoptotic genes under conditions of oxidative stress. FOXO3a is regulated by the Akt kinase which phosphorylates FOXO3a on serine 253, 315, and on threonine 32 residues. Modified FOXO3a is recognized by the 14-3-3 proteins which export FOXO3a to the cytoplasm, and consequently terminates its transcriptional activity. FOXO3a may also be regulated by lysine methylation in neuronal cells.
References
Product Information
Format Purified
Control
  • Rat liver tissue lysate.
Presentation Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application Anti-FOXO3a/FKHRL1 Antibody, clone 15F7.2 is a Mouse Monoclonal Antibody for detection of FOXO3a/FKHRL1 also known as Forkhead box protein O3, AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1 & has been validated in WB & IHC.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application Notes Immunohistochemistry Analysis: A 1:300 dilution from a representative lot detected FOXO3a/FKHRL1 in human thyroid gland tissue and human breast cancer tissue.
Biological Information
Immunogen GST-tagged recombinant protein corresponding to human FOX03a.
Clone 15F7.2
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Isotype IgG1κ
Species Reactivity
  • Human
  • Rat
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • FOXO3
  • FKHRL1
  • FOXO3A
Purification Method Protein G Purified
UniProt Number
Molecular Weight ~71 kDa observed. An uncharacterized band may be observed at ~28 kDa in some tissue lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in rat liver tissue lysate.

Western Blot Analysis: 0.2 µg/mL of this antibody detected FOXO3a/FKHRL1 in 10 mg of rat liver tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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