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Anti-PARN, clone 7A7.1 (mouse monoclonal)

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货号：05-1238

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB, ICC M Purified Monoclonal Antibody
Description
Catalogue Number 05-1238
Description Anti-PARN Antibody, clone 7A7.1
Alternate Names
  • Poly(A)-specific ribonuclease PARN
  • Deadenylating nuclease
  • Deadenylation nuclease
  • Polyadenylate-specific ribonuclease
Background Information Poly(A) ribonuclease (PARN) is a exoribonuclease responsible for the deadenylation of mRNA. This protein is constitutively expressed in virtually all mammalian tissues. With a critical role in the post-transcriptional control of gene expression PARN consists of two RNA-binding domains and one catalytic domain. Although PARN has a preference for poly(A), data suggests the protein can also degrade poly(U).
References
Product Information
Format Purified
Control
  • A431 cell lysate
Presentation Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
Applications
Application Use Anti-PARN Antibody, clone 7A7.1 (Mouse Monoclonal Antibody) validated in WB, ICC to detect PARN also known as Poly(A)-specific ribonuclease PARN, Deadenylating nuclease.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application Notes Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected PARN, clone 7A7.1 in HeLa cells.
Biological Information
Immunogen Linear peptide corresponding to human PARN.
Clone 7A7.1
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Isotype IgMκ
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary Summary: The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Gene Symbol
  • PARN
  • DAN
Purification Method Ion Exchange
UniProt Number
UniProt Summary FUNCTION: 3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization.

CATALYTIC SCTIVITY: Exonucleolytic cleavage of poly(A) to 5'-AMP.

COFACTOR: Divalent metal cations. Mg2+ is the most probable.

SUBUNIT STRUCTURE: Homodimer. Interacts with KHSRP and CELF1/CUGBP1. Found in a mRNA decay complex with RENT1, RENT2 and RENT3B.

SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Nucleus › nucleolus. Note: Some nuclear fraction is nucleolar.

TISSUE SPECIFICITY: Ubiquitous.

SEQUENCE SIMILARITIES: Belongs to the CAF1 family. Contains 1 R3H domain.
Molecular Weight ~80 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected PARN on 10 µg of A431 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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