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Anti-Serotonin (Rabbit Polyclonal)

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号:AB125

牌:Millipore 密理博

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Replacement Information

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
R IHC Rb Serum Polyclonal Antibody
Description
Catalogue Number AB125
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Serotonin Antibody
Alternate Names
  • 5-HT
References
Product Information
Format Serum
Presentation Rabbit antiserum. Liquid with 0.05% sodium azide.
Applications
Application Anti-Serotonin Antibody detects level of Serotonin & has been published & validated for use in IH.
Key Applications
  • Immunohistochemistry
Application Notes Immunohistochemistry: 1:1,000-1:2,500 by ABC or enhanced detection. Glutaraldehyde fixation is required. Antibody will not react with non-glutaraldehyde treated tissues. Blocking should be with serum or BSA containing 0.1-0.2% triton X-100; remove all blocking an detergents during primary antibody incubation. Dilute only in PBS or TBS for best results. For fluorescent detection antibody dilution must be reduced, but it is not recommended because of glutaraldehyde autofluorescence.



Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen 5-Hydroxytryptamine-glutaraldehyde-Poly-lysine
Host Rabbit
Specificity Serotonin (5-Hydroxytryptamine)

The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:



Compound Cross-reactivity

5-Hydroxytryptamine-G-PL 1

5-Methoxytryptamine-G-PL 1/25

Tryptamine-G-PL 1/60

5-Hydroxytryptophan-G-PL 1/175

5-Hydroxytryptamine 1/1150

Tryptophan-G-PL 1/3400

5-Methoxytryptophan-G-PL 1/19800



The antisera was also tested for specificity using the free-floating PAP technique on rat

raphe nuclei.



Abbreviations:



(G) Glutaraldehyde

(=) Non-reduced conjugate

(PL) Poly-lysine
Species Reactivity
  • Rat
Antibody Type Polyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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