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Anti-IL-18, clone 12E7.1 (mouse monoclonal)

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号:04-1585

牌:Millipore 密理博

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Replacement Information

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M IHC, FC M Purified Monoclonal Antibody
Description
Catalogue Number 04-1585
Description Anti-IL-18 Antibody, clone 12E7.1
Alternate Names
  • interleukin 18 (interferon-gamma-inducing factor)
  • IL-1 gamma
  • IFN-gamma-inducing factor
  • iboctadekin
  • interleukin-18
  • Interleukin-1 gamma
  • Iboctadekin
  • Interferon gamma-inducing factor
Background Information IL-18 has been shown to be a pro-inflammatory cytokine that induces interferon-gamma. IL-18 has also been found to have the ability to activate nuclear factor-kappaB (NFkB), express Fas ligand, induce CC and CXC chemokines, and also has been found to increase the production of viable human immunodeficiency virus. IL-18 has been linked to syndromes displaying symptoms of chronic low-levels of inflammation. IL-18 also seems to have some control over homeostasis and behavior in the central nervous system as well as neuroinflammatory activity.
References
Product Information
Format Purified
Control
  • PC3 cells
Presentation Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
Applications
Application Anti-IL-18 Antibody, clone 12E7.1 is an antibody against IL-18 for use in IH, FC.
Key Applications
  • Immunohistochemistry
  • Flow Cytometry
Application Notes Immunohistochemistry Analysis: 1:250 from a previous lot detected IL-18 in human tonsil tissue.

Immunohistochemistry Analysis: 1:250 from a previous lot detected IL-18 in inflamed skin and normal human spleen tissues.
Biological Information
Immunogen KLH-conjugated linear peptide corresponding to human IL-18.
Clone 12E7.1
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Isotype IgMκ
Species Reactivity
  • Human
  • Mouse
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. [provided by RefSeq].
Gene Symbol
  • IL-18
  • IL18
  • IGIF
  • IL-18
  • IL1F4
  • IL-1g
Purification Method Ion Exchange
UniProt Number
UniProt Summary FUNCTION: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.

SUBCELLULAR LOCATION: Secreted.

SEQUENCE SIMILARITIES: Belongs to the IL-1 family.
Molecular Weight ~24 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Flow Cytometry in PC3 cells.

Flow Cytometry Analysis: 1 µg of this antibody detected IL-18 in PC3 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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