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Anti-phospho-Threonine, clone 20H6.1 (mouse monoclonal)

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货号：05-1923

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M, A WB, ICC M Purified Monoclonal Antibody
Description
Catalogue Number 05-1923
Description Anti-phospho-Threonine Antibody, clone 20H6.1
Alternate Names
  • Threonine
Background Information Protein phosphorylation is normally associated with protein modification in signaling pathways and may be useful in pathophysiology and treatment of major neuropsychiatric disorders. Antibodies against phospho-threonine have been used to establish stability of the phosphorylated forms of proteins that may be involved in many different types of signaling. Phospho-threonine antibody is used to measure phosphorylation levels of PSII reaction center proteins D1, D3 and chlorophyll a binding protein CP43.
References
Product Information
Format Purified
Control
  • Calyculin/okadaic acid treated and untreated A431 cell lysates.
Presentation Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application This phospho-Threonine antibody is validated for use in WB & ICC for the detection of the phospho-Threonine protein.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application Notes Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated Threonine from 100 µg of A431 cells stimulated with calyculin/okadaic acid.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Threonine in A431, NIH/3T3, and HeLa cells.
Biological Information
Immunogen KLH-conjugated linear peptide corresponding to Threonine.
Clone 20H6.1
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Isotype IgG2bκ
Species Reactivity
  • Human
  • Mouse
  • All
Species Reactivity Note Demonstrated to react with human and mouse. Predicted to react with all vertebrates based on 100% sequence homology.
Antibody Type Monoclonal Antibody
Gene Symbol
  • THR
Purification Method Protein G
Molecular Weight Various observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in calyculin/okadaic acid treated and untreated A431 cell lysates.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Threonine on 10 µg of calyculin/okadaic acid treated and untreated A431 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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