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Anti-EF1a, clone CBP-KK1

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货号：05-235

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
Bact, Ma, Yeast (S. cerevisiae) IP, WB M Purified Monoclonal Antibody
Description
Catalogue Number 05-235
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-EF1α Antibody, clone CBP-KK1
Alternate Names
  • CTCL tumor antigen
  • EF1a-like protein
  • Elongation factor 1 A-1
  • Elongation factor Tu
  • Leukocyte receptor cluster member 7
  • cervical cancer suppressor 3
  • elongation factor 1 alpha subunit
  • elongation factor 1-alpha
  • eukaryotic translation elongation factor 1 alpha 1
  • eukaryotic translation elongation factor 1 alpha 1-like 14
  • glucocorticoid receptor AF-1 specific elongation factor
  • leukocyte receptor cluster (LRC) member 7
  • prostate tumor-inducing protein 1
  • translation elongation factor 1 alpha 1-like 14
Background Information Human eukaryotic translation elongation factor 1 alpha has two isoforms, alpha 1 and alpha 2. The alpha 1 isoform gene encodes a subunit of the elongation factor-1 complex which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. The alpha 1 isoform is expressed in brain, placenta, lung, liver, kidney, and pancreas. The other isoform (alpha 2) is expressed in brain, heart and skeletal muscle.
The alpha 1 isoform is identified as an autoantigen in 66% of patients with Felty syndrome. The gene for the alpha 1 isoform has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. The gene for the alpha 2 may be critical in the development of ovarian cancer.
References
Product Information
Format Purified
Control
  • Jurkat cell lysate.
    Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 µL of 2-mercaptoethanol per 100 µL of lysate and boil for 5 minutes to reduce the preparation. Use 20 µg per lane for minigels.
Presentation Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide.
Applications
Application Detect EF1α using this Anti-EF1α Antibody, clone CBP-KK1 validated for use in IP & WB.
Key Applications
  • Immunoprecipitation
  • Western Blotting
Application Notes Immunoprecipitation:
4 µg of a previous lot immunoprecipitated EF1α from an A431 RIPA cell lysate.
Biological Information
Immunogen Crude calmodulin-binding proteins from Trypanosoma brucei isolated using calmodulin-affinity chromatography. Clone CBP-KK1.
Clone CBP-KK1
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity This antibody recognizes EF-1α (Elongation factor-1 alpha), Mr 53 kDa. Occasionally, a 38 kDa protein is also detected in A431 cell lysate.
Isotype IgG1κ
Species Reactivity
  • Bacteria
  • Mammals
  • Yeast (S. cerevisiae)
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes.
Gene Symbol
  • EEF1A1
  • eEF1A-1
  • FLJ25721
  • EF-1-alpha-1
  • EEF-1
  • HNGC:16303
  • ALPHA
  • CCS-3
  • PTI1
  • MGC131894
  • EEF1A
  • CCS3
  • MGC102687
  • EF-Tu
  • MGC16224
  • GRAF-1EF
  • LENG7
  • EF1A
Purification Method Protein G Chromatography
UniProt Number
UniProt Summary FUNCTION: SwissProt: P68104 # This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
SIZE: 462 amino acids; 50141 Da
SUBUNIT: Found in a nuclear export complex with XPO5, EEF1A1, Ran and aminoacylated tRNA. Interacts with XPO5.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle.
SIMILARITY: SwissProt: P68104 ## Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.
Molecular Weight 53 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Routinely evaluated on RIPA lysates from non-stimulated A431 cells, human Jurkat or mouse 3T3/A31.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected EF1α in RIPA lysates from non-stimulated A431 cells. Previous lots detected EF1α in human Jurkat and mouse 3T3/A31 RIPA cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size 200 µg
Transport Information
Supplemental Information
Specifications

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