Anti-RuvB-like 2 (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	WB	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	06-1300
Description	Anti-RuvB-like 2 Antibody
Alternate Names	RuvB-like 2 (E. coli) TIP60-associated protein 54-beta 48 kDa TATA box-binding protein-interacting protein INO80 complex subunit J 51 kDa erythrocyte cytosolic protein Reptin52 RuvB (E coli homolog)-like 2 REPTIN ruvB-like 2 Reptin 52 TBP-interacting protein, 48-KD Repressing pontin 52 erythrocyte cytosolic protein, 51-KD 48 kDa TBP-interacting protein
Background Information	RuvB-like 2 (RUVBL2), also called Reptin 52 and TIP49B, is a DNA helicase of the AAA+ ATPase family known to be involved in double-strand break repair. The RuvB-Like 2 is the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. In mammalian cells, functional analysis of the RevB-like 2 protein has shown it functions as both and DNA-stimulated ATPase and an ATP-dependent DNA helicase. As a component of the NuA4 histone acetyltransferase complex this protein acetylates histones H4 and H2A to activate transcription. These changes appear to alter nucleosome-DNA interactions and promote the interactions of other proteins that activate transcription. Activation by this complex may be required for activation of genes associated with with tumor suppressor mediated growth arrest, apoptosis, DNA repair, as well as oncogene and proto-oncogene mediated growth induction.

Description

Catalogue Number

06-1300

Description

Anti-RuvB-like 2 Antibody

Alternate Names

RuvB-like 2 (E. coli)
TIP60-associated protein 54-beta
48 kDa TATA box-binding protein-interacting protein
INO80 complex subunit J
51 kDa erythrocyte cytosolic protein
Reptin52
RuvB (E coli homolog)-like 2
REPTIN
ruvB-like 2
Reptin 52
TBP-interacting protein, 48-KD
Repressing pontin 52
erythrocyte cytosolic protein, 51-KD
48 kDa TBP-interacting protein

Background Information

RuvB-like 2 (RUVBL2), also called Reptin 52 and TIP49B, is a DNA helicase of the AAA+ ATPase family known to be involved in double-strand break repair. The RuvB-Like 2 is the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. In mammalian cells, functional analysis of the RevB-like 2 protein has shown it functions as both and DNA-stimulated ATPase and an ATP-dependent DNA helicase. As a component of the NuA4 histone acetyltransferase complex this protein acetylates histones H4 and H2A to activate transcription. These changes appear to alter nucleosome-DNA interactions and promote the interactions of other proteins that activate transcription. Activation by this complex may be required for activation of genes associated with with tumor suppressor mediated growth arrest, apoptosis, DNA repair, as well as oncogene and proto-oncogene mediated growth induction.

Product Information
Format	Affinity Purified
Control	Human testis tissue lysate
Presentation	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Affinity Purified

Control

Human testis tissue lysate

Presentation

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	Anti-RuvB-like 2 Antibody is a rabbit polyclonal antibody for detection of RuvB-like 2 also known as 51 kDa erythrocyte cytosolic protein, Reptin52, RuvB (E coli homolog)-like 2, TBP-interacting protein & has been validated in WB.
Key Applications	Western Blotting

Applications

Application

Anti-RuvB-like 2 Antibody is a rabbit polyclonal antibody for detection of RuvB-like 2 also known as 51 kDa erythrocyte cytosolic protein, Reptin52, RuvB (E coli homolog)-like 2, TBP-interacting protein & has been validated in WB.

Key Applications

Western Blotting

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to human RuvB-like 2.
Host	Rabbit
Species Reactivity	Human Mouse Rat
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_006657
Entrez Gene Summary	This gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation. [provided by RefSeq].
Gene Symbol	ECP-51 TIP48 TAP54-beta ECP51 INO80 TIH2 RVB2 Rvb2 TIP49b
Purification Method	Affinity Purfied
UniProt Number	Q9Y230
UniProt Summary	FUNCTION: Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL2 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2. CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate. SUBUNIT STRUCTURE: Forms a multimeric complex with RUVBL1. Interacts with the transcriptional activation domain of MYC. Interacts With ATF2. Component of the RNA polymerase II holoenzyme complex. May also act to bridge the LEF1/TCF1-CTNNB1 complex and TBP. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. The NuA4 complex interacts with MYC and the adenovirus E1A protein. RUVBL2 interacts with EP400. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41. Interacts with NPAT. Component of the chromatin-remodeling INO80 complex, at least composed of ACTL6A, ACTR5, ACTR8, RVBL1, RVBL2, INO80, INO80B, INO80C, INO80D and INO80E. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, IN80C, KIAA1267, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with IGHMBP2. SUBCELLULAR LOCATION: Nucleus matrix. Nucleus › nucleoplasm. Cytoplasm. Membrane. Note: Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol. Although it is also present in the cytoplasm and associated with the cell membranes. TISSUE SPECIFICTY: Ubiquitously expressed. Highly expressed in testis and thymus. DOMAIN: The C-terminal domain is required for association with ATF2. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. SEQUENCE SIMILARITIES: Belongs to the ruvB family. SEQUENCE CAUTION: The sequence AAD34041.1 differs from that shown. Reason: Frameshift at position 401. The sequence AAH08355.1 differs from that shown. Reason: Frameshift at position 191.
Molecular Weight	~51 kDa observed

Biological Information

Immunogen

KLH-conjugated linear peptide corresponding to human RuvB-like 2.

Host

Rabbit

Species Reactivity

Human
Mouse
Rat

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_006657

Entrez Gene Summary

This gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation. [provided by RefSeq].

Gene Symbol

ECP-51
TIP48
TAP54-beta
ECP51
INO80
TIH2
RVB2
Rvb2
TIP49b

Purification Method

Affinity Purfied

UniProt Number

Q9Y230

UniProt Summary

FUNCTION: Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL2 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2.

CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate.

SUBUNIT STRUCTURE: Forms a multimeric complex with RUVBL1. Interacts with the transcriptional activation domain of MYC. Interacts With ATF2. Component of the RNA polymerase II holoenzyme complex. May also act to bridge the LEF1/TCF1-CTNNB1 complex and TBP. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. The NuA4 complex interacts with MYC and the adenovirus E1A protein. RUVBL2 interacts with EP400. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41. Interacts with NPAT. Component of the chromatin-remodeling INO80 complex, at least composed of ACTL6A, ACTR5, ACTR8, RVBL1, RVBL2, INO80, INO80B, INO80C, INO80D and INO80E. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, IN80C, KIAA1267, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with IGHMBP2.

SUBCELLULAR LOCATION: Nucleus matrix. Nucleus › nucleoplasm. Cytoplasm. Membrane. Note: Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol. Although it is also present in the cytoplasm and associated with the cell membranes.

TISSUE SPECIFICTY: Ubiquitously expressed. Highly expressed in testis and thymus.

DOMAIN: The C-terminal domain is required for association with ATF2.

PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.

SEQUENCE SIMILARITIES: Belongs to the ruvB family.

SEQUENCE CAUTION: The sequence AAD34041.1 differs from that shown. Reason: Frameshift at position 401.

The sequence AAH08355.1 differs from that shown. Reason: Frameshift at position 191.

Molecular Weight

~51 kDa observed

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in human testis tissue lysate. Western Blot Analysis: 1:1,000 dilution of this antibody detected RuvB-like 2 on 10 µg of human testis tissue lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in human testis tissue lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected RuvB-like 2 on 10 µg of human testis tissue lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-RuvB-like 2 (rabbit polyclonal)

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