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Anti-SRp75 (rabbit polyclonal)

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号:06-1367

牌:Millipore 密理博

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Replacement Information

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB Rb Purified Polyclonal Antibody
Description
Catalogue Number 06-1367
Description Anti-SRp75 Antibody
Alternate Names
  • serine/arginine-rich splicing factor 4
  • splicing factor, arginine/serine-rich 4
  • SR splicing factor 4
  • Pre-mRNA-splicing factor SRP75
Background Information SRp75, also known as Serine/arginine-rich splicing factor 4, is an essential splicing factor that is a member of the SR family of proteins. This protein is a component of the pre-mRNA splicing complex and is believed to play a role in alternative splice site selection. Consistent with this excess levels of SRp75 have been shown to play a role in alternative splicing of CD45. SRp75 is normally found in the nucleus and is known to be hyperphosphorylated at serine residues in its RS domain.
References
Product Information
Format Purified
Control
  • L6 cell lysate
Presentation Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application Anti-SRp75 Antibody is a rabbit polyclonal antibody for detection of SRp75 also known as serine/arginine-rich splicing factor 4, SR splicing factor 4, Pre-mRNA-splicing factor SRP75 & has been validated in WB.
Key Applications
  • Western Blotting
Application Notes Immunoprecipitation Analysis: A reprentative lot of this antibody was used by an independent laboratory in IP. (Jiang, K., et al. (2009). Endocrinology. 150(5):2087-2097.)
Biological Information
Immunogen KLH-conjugated linear peptide corresponding to human SRp75.
Host Rabbit
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene encodes a member of the arginine/serine-rich splicing factor family. The encoded protein likely functions in mRNA processing. [provided by RefSeq].
Gene Symbol
  • SRP001LB
  • SRP75
  • SFRS4
  • SRSF4
Purification Method Protein A
UniProt Number
UniProt Summary FUNCTION: A probable role in alternative splice site selection during pre-mRNA splicing.

SUBUNIT STRUCTURE: Found in a pre-mRNA splicing complex with SRSF4/SFRS4, SRSF5/SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2. Interacts with PNN.

SUBCELLULAR LOCATION: Nucleus speckle

PTM: Extensively phosphorylated on serine residues in the RS domain.

SEQUENCE SIMILARITIES: Belongs to the splicing factor SR family.

Contains 2 RRM (RNA recognition motif) domains.
Molecular Weight ~ 62 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in L6 cell lysate.

Western Blot Analysis: 1:500 dilution of this antibody detected SRp75 on 10 µg of L6 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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