Anti-phospho MRE11 (Thr636) (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	06-1374
Description	Anti-phospho MRE11 (Thr636) Antibody
Alternate Names	MRE11 meiotic recombination 11 homolog A (S. cerevisiae) meiotic recombination (S. cerevisiae) 11 homolog A double-strand break repair protein MRE11A Meiotic recombination 11 homolog A Meiotic recombination 11 homolog 1 DNA recombination and repair protein MRE11 homolog 1 endo/exonuclease Mre11 MRE11 homolog A AT-like disease
Background Information	MRE11 is is a nuclear protein involved the DNA damge repair and recombination. This protein is part of the MRN complex composed of three main components: Mre11, Rad50, and Nbs1/Xrs2. This protein complex is involved in signaling pathways that lead to both checkpoint activation and DNA repair. Mutations and defects in expression of this protein can lead to genomic instability, telomere shortening, aberrant meiosis and hypersensitivity to DNA damage.

Description

Catalogue Number

06-1374

Description

Anti-phospho MRE11 (Thr636) Antibody

Alternate Names

MRE11 meiotic recombination 11 homolog A (S. cerevisiae)
meiotic recombination (S. cerevisiae) 11 homolog A
double-strand break repair protein MRE11A
Meiotic recombination 11 homolog A
Meiotic recombination 11 homolog 1
DNA recombination and repair protein
MRE11 homolog 1
endo/exonuclease Mre11
MRE11 homolog A
AT-like disease

Background Information

MRE11 is is a nuclear protein involved the DNA damge repair and recombination. This protein is part of the MRN complex composed of three main components: Mre11, Rad50, and Nbs1/Xrs2. This protein complex is involved in signaling pathways that lead to both checkpoint activation and DNA repair. Mutations and defects in expression of this protein can lead to genomic instability, telomere shortening, aberrant meiosis and hypersensitivity to DNA damage.

Product Information
Format	Affinity Purified
Control	Anisomycin untreated and treated HEK293 cell lysates
Presentation	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Affinity Purified

Control

Anisomycin untreated and treated HEK293 cell lysates

Presentation

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	Anti-phospho MRE11 (Thr636) Antibody is a rabbit polyclonal antibody for detection of phospho MRE11 (Thr636) also known as MRE11 meiotic recombination 11 homolog A (S. cerevisiae), has been validated in WB.
Key Applications	Western Blotting

Applications

Application

Anti-phospho MRE11 (Thr636) Antibody is a rabbit polyclonal antibody for detection of phospho MRE11 (Thr636) also known as MRE11 meiotic recombination 11 homolog A (S. cerevisiae), has been validated in WB.

Key Applications

Western Blotting

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to human MRE11 phosphorylated at Thr636.
Epitope	Phosphorylated Thr636
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	This antibody recognizes MRE11 phosphorylated at Thr636.
Species Reactivity	Human
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_005581
Entrez Gene Summary	This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq].
Gene Symbol	MRE11A MRE11 MRE11B ATLD HNGS1
Modifications	Phosphorylation
Purification Method	Affinity Purfied
UniProt Number	P49959
UniProt Summary	FUNCTION: Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation. COFACTOR: Manganese (By similarity). SUBUNIT STRUCTURE: Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11A and NBN (By similarity). Interacts with DCLRE1C/Artemis and DCLRE1B/Apollo. SUBCELLULAR LOCATION: Nucleus (By similarity). Note: Localizes to discrete nuclear foci after treatment with genotoxic agents (By similarity). PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. INVOLVEMENT IN DISEASE: Defects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course. MISCELLANEOUS: In case of infection by adenovirus E4, the MRN complex is inactivated and degraded by viral oncoproteins, thereby preventing concatenation of viral genomes in infected cells. SEQUENCE SIMILARITIES: Belongs to the MRE11/RAD32 family.
Molecular Weight	~85 kDa observed

Biological Information

Immunogen

KLH-conjugated linear peptide corresponding to human MRE11 phosphorylated at Thr636.

Epitope

Phosphorylated Thr636

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

This antibody recognizes MRE11 phosphorylated at Thr636.

Species Reactivity

Human

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_005581

Entrez Gene Summary

This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq].

Gene Symbol

MRE11A
MRE11
MRE11B
ATLD
HNGS1

Modifications

Phosphorylation

Purification Method

Affinity Purfied

UniProt Number

P49959

UniProt Summary

FUNCTION: Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.

COFACTOR: Manganese (By similarity).

SUBUNIT STRUCTURE: Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11A and NBN (By similarity). Interacts with DCLRE1C/Artemis and DCLRE1B/Apollo.

SUBCELLULAR LOCATION: Nucleus (By similarity). Note: Localizes to discrete nuclear foci after treatment with genotoxic agents (By similarity).

PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.

INVOLVEMENT IN DISEASE: Defects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course.

MISCELLANEOUS: In case of infection by adenovirus E4, the MRN complex is inactivated and degraded by viral oncoproteins, thereby preventing concatenation of viral genomes in infected cells.

SEQUENCE SIMILARITIES: Belongs to the MRE11/RAD32 family.

Molecular Weight

~85 kDa observed

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in anisomycin untreated and treated HEK293 cell lysates. Western Blot Analysis: 1 µg/mL of this antibody detected MRE11 on 10 µg of anisomycin untreated and treated HEK293 cell lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in anisomycin untreated and treated HEK293 cell lysates.

Western Blot Analysis: 1 µg/mL of this antibody detected MRE11 on 10 µg of anisomycin untreated and treated HEK293 cell lysates.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-phospho MRE11 (Thr636) (rabbit polyclonal)

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