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Anti-phospho-GSK3ß (Ser9), clone 2D3 (mouse monoclonal IgG1k)

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货号：05-643

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M, R EMSA, WB M Purified Monoclonal Antibody
Description
Catalogue Number 05-643
Replaces 04-1075
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-phospho-GSK3β (Ser9) Antibody, clone 2D3
References
Product Information
Format Purified
Presentation 100µg of thiophilic and size exclusion chromatography purified mouse IgG1κ in 100µL of antibody storage buffer (PBS with PEG, Sucrose, and 0.09% sodium azide) and 50% glycerol.
Applications
Application Anti-phospho-GSK3β (Ser9) Antibody, clone 2D3 is an antibody against phospho-GSK3β (Ser9) for use in EMSA & WB.
Key Applications
  • Electrophoretic Mobility Shift Assay
  • Western Blotting
Biological Information
Immunogen peptide corresponding to amino acids 6-12 (RTT[pS]AE) of human GSK3beta
Clone 2D3
Host Mouse
Specificity GSK3beta
Isotype IgG1κ
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK3A; MIM 606784) and beta, show a high degree of amino acid homology (Stambolic and Woodgett, 1994 [PubMed 7980435]). GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation (Plyte et al., 1992 [PubMed 1333807]).[supplied by OMIM]
Gene Symbol
  • GSK3B
Modifications
  • Phosphorylation
Purification Method Thiophilic adsorption chromatography
UniProt Number
UniProt Summary FUNCTION: SwissProt: P49841 # Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA.
SIZE: 420 amino acids; 46744 Da
SUBUNIT: Monomer (By similarity). Interacts with NIN and CABYR.
TISSUE SPECIFICITY: Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney.
PTM: Phosphorylated by AKT1 and ILK1.
SIMILARITY: SwissProt: P49841 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily. & Contains 1 protein kinase domain.
Molecular Weight 46kDa (GSK3β) and 51kDa (GSK3α)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance routinely evaluated by immunoblot on L6 cells stimulated with IGF-1
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions 2 years at -20°C
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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