Anti-phospho-Nibrin/nbs1 (Ser343), clone 2G2E3

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	IP	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

Purified

Monoclonal Antibody

Description
Catalogue Number	05-663
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-phospho-Nibrin/Nbs1 (Ser343) Antibody, clone 2G2E3

Description

Catalogue Number

05-663

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-phospho-Nibrin/Nbs1 (Ser343) Antibody, clone 2G2E3

Product Information
Format	Purified
Presentation	0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%

Product Information

Format

Purified

Presentation

0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%

Applications
Application	Detect phospho-Nibrin/Nbs1 (Ser343) with Anti-phospho-Nibrin/Nbs1 (Ser343) Antibody, clone 2G2E3 (Mouse Monoclonal Antibody), that has been demonstrated to work in IP.
Key Applications	Immunoprecipitation

Applications

Application

Detect phospho-Nibrin/Nbs1 (Ser343) with Anti-phospho-Nibrin/Nbs1 (Ser343) Antibody, clone 2G2E3 (Mouse Monoclonal Antibody), that has been demonstrated to work in IP.

Key Applications

Immunoprecipitation

Biological Information
Immunogen	Peptide corresponding to amino acids 337-350 of human Nibrin/nbs1
Clone	2G2E3
Host	Mouse
Specificity	Nibrin/nbs1 phosphorylated on serine 343
Isotype	IgG2aκ
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_002485
Entrez Gene Summary	Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Gene Symbol	NBN P95 MGC87362 Nibrin FLJ10155 ATV AT-V2 NBS AT-V1 NBS1
Modifications	Phosphorylation
Purification Method	Protein A chromatography
UniProt Number	O60934
UniProt Summary	FUNCTION: SwissProt: O60934 # Component of the MRE11/RAD50/NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. SIZE: 754 amino acids; 84959 Da SUBUNIT: Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50 and MRE11A (By similarity). Interacts with histone H2AFX this requires phosphorylation of H2AFX on 'Ser-139'. Interacts with TERF2 and with KPNA2. SUBCELLULAR LOCATION: Nucleus (By similarity). Note=Localizes to discrete nuclear foci after treatment with genotoxic agents (By similarity). TISSUE SPECIFICITY: Ubiquitous. Expressed at high levels in testis. DOMAIN: SwissProt: O60934 The FHA and BRCT domains are likely to have a crucial role for both binding to histone H2AFX and for relocalization of MRE11/RAD50 complex to the vicinity of DNA damage. & The C-terminal domain contains a MRE11-binding site, and this interaction is required for the nuclear localization of the MRN complex. & The EEXXXDDL motif at the C-terminus is required for the interaction with ATM and its recruitment to sites of DNA damage and promote the phosphorylation of ATM substrates, leading to the events of DNA damage response. PTM: Phosphorylated by ATM in response of ionizing radiation, and such phosphorylation is responsible intra-S phase checkpoint control and telomere maintenance. DISEASE: SwissProt: O60934 # Defects in NBN are the cause of Nijmegen breakage syndrome (NBS) [MIM:251260]. NBS is an autosomal recessive syndrome characterized by chromosomal instability, radiation sensitivity, microcephaly, growth retardation, immunodeficiency and predisposition to cancer, particularly to lymphoid malignancies. & Defects in NBN are a cause of genetic susceptibility to breast cancer (BC) [MIM:114480]. BC is an extremely common malignancy, affecting one in eight women during their lifetime. A positive family history has been identified as major contributor to risk of development of the disease, and this link is striking for early-onset breast cancer. & Defects in NBN may be associated with aplastic anemia [MIM:609135]. Aplastic anemia is a disease of bone-marrow failure characterized by peripheral pancytopenia and marrow hypoplasia. Most of the cases of aplastic anemia are idiopathic, some are familial and some are due to a viral infection or to exposure to chemicals and radiation. & Defects in NBN might play a role in the pathogenesis of childhood acute lymphoblastic leukemia (ALL). SIMILARITY: Contains 1 BRCT domain. & Contains 1 FHA domain. MISCELLANEOUS: In case of infection by adenovirus E4, the MRN complex is inactivated and degraded by viral oncoproteins, thereby preventing concatenation of viral genomes in infected cells.
Molecular Weight	~100kDa

Biological Information

Immunogen

Peptide corresponding to amino acids 337-350 of human Nibrin/nbs1

Clone

2G2E3

Host

Mouse

Specificity

Nibrin/nbs1 phosphorylated on serine 343

Isotype

IgG2aκ

Species Reactivity

Human

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_002485

Entrez Gene Summary

Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Gene Symbol

NBN
P95
MGC87362
Nibrin
FLJ10155
ATV
AT-V2
NBS
AT-V1
NBS1

Modifications

Phosphorylation

Purification Method

Protein A chromatography

UniProt Number

O60934

UniProt Summary

FUNCTION: SwissProt: O60934 # Component of the MRE11/RAD50/NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability.

SIZE: 754 amino acids; 84959 Da

SUBUNIT: Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50 and MRE11A (By similarity). Interacts with histone H2AFX this requires phosphorylation of H2AFX on 'Ser-139'. Interacts with TERF2 and with KPNA2.

SUBCELLULAR LOCATION: Nucleus (By similarity). Note=Localizes to discrete nuclear foci after treatment with genotoxic agents (By similarity).

TISSUE SPECIFICITY: Ubiquitous. Expressed at high levels in testis.

DOMAIN: SwissProt: O60934 The FHA and BRCT domains are likely to have a crucial role for both binding to histone H2AFX and for relocalization of MRE11/RAD50 complex to the vicinity of DNA damage. & The C-terminal domain contains a MRE11-binding site, and this interaction is required for the nuclear localization of the MRN complex. & The EEXXXDDL motif at the C-terminus is required for the interaction with ATM and its recruitment to sites of DNA damage and promote the phosphorylation of ATM substrates, leading to the events of DNA damage response.

PTM: Phosphorylated by ATM in response of ionizing radiation, and such phosphorylation is responsible intra-S phase checkpoint control and telomere maintenance.

DISEASE: SwissProt: O60934 # Defects in NBN are the cause of Nijmegen breakage syndrome (NBS) [MIM:251260]. NBS is an autosomal recessive syndrome characterized by chromosomal instability, radiation sensitivity, microcephaly, growth retardation, immunodeficiency and predisposition to cancer, particularly to lymphoid malignancies. & Defects in NBN are a cause of genetic susceptibility to breast cancer (BC) [MIM:114480]. BC is an extremely common malignancy, affecting one in eight women during their lifetime. A positive family history has been identified as major contributor to risk of development of the disease, and this link is striking for early-onset breast cancer. & Defects in NBN may be associated with aplastic anemia [MIM:609135]. Aplastic anemia is a disease of bone-marrow failure characterized by peripheral pancytopenia and marrow hypoplasia. Most of the cases of aplastic anemia are idiopathic, some are familial and some are due to a viral infection or to exposure to chemicals and radiation. & Defects in NBN might play a role in the pathogenesis of childhood acute lymphoblastic leukemia (ALL).

SIMILARITY: Contains 1 BRCT domain. & Contains 1 FHA domain.

MISCELLANEOUS: In case of infection by adenovirus E4, the MRN complex is inactivated and degraded by viral oncoproteins, thereby preventing concatenation of viral genomes in infected cells.

Molecular Weight

~100kDa

Product Usage Statements
Quality Assurance	routinely evaluated by immunoprecipitating nibrin-containing immune complexes collected with anti-Nibrin (Catalog #07-317) from RIPA lysates of etoposide-treated, but not untreated, Jurkat cells
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

routinely evaluated by immunoprecipitating nibrin-containing immune complexes collected with anti-Nibrin (Catalog #07-317) from RIPA lysates of etoposide-treated, but not untreated, Jurkat cells

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	2 years at -20°C

Storage and Shipping Information

Storage Conditions

2 years at -20°C

Packaging Information
Material Size	200 µg

Packaging Information

Material Size

200 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-phospho-Nibrin/nbs1 (Ser343), clone 2G2E3

浏览了该商品的用户最终购买

浏览了该商品的用户还浏览了

特卖

重要规格表

我要咨询