Anti-PI3 Kinase-C2ß (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, Chp, Rhesus Macaque	WB, IHC	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, Chp, Rhesus Macaque

WB, IHC

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	07-2170
Description	Anti-PI3 Kinase-C2β Antibody
Alternate Names	phosphoinositide-3-kinase, class 2, beta polypeptide1 2 phosphatidylinositol 3-kinase C2 domain-containing beta polypeptide PI3K-C2beta PTDINS-3-kinase C2 beta Phosphoinositide 3-kinase-C2-beta phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit beta PtdIns-3-kinase C2 subunit beta PI3K-C2-beta
Background Information	The class II phosphoinositide 3-kinase beta (PI3K-C2) is a member of the PI3K family of enzymes and is thought to play a role in the creation of 3’ phosphoinositides after the activation of polypeptide growth factor receptors. PI3K-C2activation during liver growth causes increased levels of phosphatidylinositol 4-phosphate in cell nuclei and may be regulated by calpain. The proline-rich motifs in the N-terminus of PI3K-C2 is thought to associate with epidermal growth factor and this could also include the Grb2 adaptor. It has also been shown that PI3K-C2 may play a role in a signaling pathway for cell migration. Recent research has also found that PI3K-C2 may be essential in T-cell activation.

Description

Catalogue Number

07-2170

Description

Anti-PI3 Kinase-C2β Antibody

Alternate Names

phosphoinositide-3-kinase, class 2, beta polypeptide1 2 phosphatidylinositol 3-kinase C2 domain-containing beta polypeptide
PI3K-C2beta
PTDINS-3-kinase C2 beta
Phosphoinositide 3-kinase-C2-beta
phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit beta
PtdIns-3-kinase C2 subunit beta
PI3K-C2-beta

Background Information

The class II phosphoinositide 3-kinase beta (PI3K-C2) is a member of the PI3K family of enzymes and is thought to play a role in the creation of 3’ phosphoinositides after the activation of polypeptide growth factor receptors. PI3K-C2activation during liver growth causes increased levels of phosphatidylinositol 4-phosphate in cell nuclei and may be regulated by calpain. The proline-rich motifs in the N-terminus of PI3K-C2 is thought to associate with epidermal growth factor and this could also include the Grb2 adaptor. It has also been shown that PI3K-C2 may play a role in a signaling pathway for cell migration. Recent research has also found that PI3K-C2 may be essential in T-cell activation.

Product Information
Format	Affinity Purified
Control	MCF-7 cell lysate
Presentation	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Affinity Purified

Control

MCF-7 cell lysate

Presentation

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	This PI3 Kinase-C2β antibody is validated for use in WB & IHC for the detection of the PI3 Kinase-C2β protein.
Key Applications	Western Blotting Immunohistochemistry
Application Notes	Immunohistochemistry Analysis: 1:300 dilution from a previous lot was used to detect PI3K-C2β in normal human spleen tissue.

Applications

Application

This PI3 Kinase-C2β antibody is validated for use in WB & IHC for the detection of the PI3 Kinase-C2β protein.

Key Applications

Western Blotting
Immunohistochemistry

Application Notes

Immunohistochemistry Analysis: 1:300 dilution from a previous lot was used to detect PI3K-C2β in normal human spleen tissue.

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to human PI3 Kinase-C2β.
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Species Reactivity	Human Chimpanzee Rhesus Macaque
Species Reactivity Note	Demonstrated to react with human. Predicted to react with chimpanzee, rhesus macaque, and orangutan based on 100% sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_002637.3
Entrez Gene Summary	The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is sensitive to low nanomolar levels of the inhibitor wortmanin. The C2 domain of this protein was shown to bind phospholipids but not Ca2+, which suggests that this enzyme may function in a calcium-independent manner. [provided by RefSeq].
Gene Symbol	C2-PI3K PIK3C2B
Purification Method	Affinity Purfied
UniProt Number	O00750
UniProt Summary	FUNCTION: Phosphorylates PtdIns and PtdIns4P with a preference for PtdIns. Does not phosphorylate PtdIns(4,5)P2. May be involved in EGF and PDGF signaling cascades. Ref.4 CATALYTIC ACTIVITY: ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate. CAFACTOR: Calcium, magnesium, or manganese. ENZYME REGULATION: Activated by GRB2. SUBUNIT STRUCTURE: Part of a complex with ERBB2 and EGFR. Part of a complex with phosphorylated EGFR and GRB2. Interacts with phosphorylated EGFR and PDGFR, maybe indirectly. Interacts with GRB2 and SHC1. SUBCELLULAR LOCATION: Microsome. Cell membrane. Cytoplasm › cytosol. Nucleus. Endoplasmic reticulum. Note: Found mostly in the microsome, but also in the plasma membrane and cytosol. Nuclear in testis. TISSUE SPECIFICITY: Expressed in columnar and transitional epithelia, mononuclear cells, and ganglion cells (at protein level). Widely expressed, with highest levels in thymus and placenta and lowest in peripheral blood, skeletal muscle and kidney. SEQUENCE SIMILARITIES: Belongs to the PI3/PI4-kinase family. Contains 1 C2 domain. Contains 1 PI3K/PI4K domain. Contains 1 PX (phox homology) domain. CAUTION: It is uncertain whether Met-1 or Met-26 is the initiator. SEQUENCE CAUTION: The sequence CAA74194.1 differs from that shown. Reason: Erroneous initiation.
Molecular Weight	~184 kDa observed. Weaker cross-reacting bands observed at ~28 and 16 kDa in some lysates.

Biological Information

Immunogen

KLH-conjugated linear peptide corresponding to human PI3 Kinase-C2β.

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Species Reactivity

Human
Chimpanzee
Rhesus Macaque

Species Reactivity Note

Demonstrated to react with human. Predicted to react with chimpanzee, rhesus macaque, and orangutan based on 100% sequence homology.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_002637.3

Entrez Gene Summary

The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is sensitive to low nanomolar levels of the inhibitor wortmanin. The C2 domain of this protein was shown to bind phospholipids but not Ca2+, which suggests that this enzyme may function in a calcium-independent manner. [provided by RefSeq].

Gene Symbol

C2-PI3K
PIK3C2B

Purification Method

Affinity Purfied

UniProt Number

O00750

UniProt Summary

FUNCTION: Phosphorylates PtdIns and PtdIns4P with a preference for PtdIns. Does not phosphorylate PtdIns(4,5)P2. May be involved in EGF and PDGF signaling cascades. Ref.4

CATALYTIC ACTIVITY: ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate.

CAFACTOR: Calcium, magnesium, or manganese.

ENZYME REGULATION: Activated by GRB2.

SUBUNIT STRUCTURE: Part of a complex with ERBB2 and EGFR. Part of a complex with phosphorylated EGFR and GRB2. Interacts with phosphorylated EGFR and PDGFR, maybe indirectly. Interacts with GRB2 and SHC1.

SUBCELLULAR LOCATION: Microsome. Cell membrane. Cytoplasm › cytosol. Nucleus. Endoplasmic reticulum. Note: Found mostly in the microsome, but also in the plasma membrane and cytosol. Nuclear in testis.

TISSUE SPECIFICITY: Expressed in columnar and transitional epithelia, mononuclear cells, and ganglion cells (at protein level). Widely expressed, with highest levels in thymus and placenta and lowest in peripheral blood, skeletal muscle and kidney.

SEQUENCE SIMILARITIES: Belongs to the PI3/PI4-kinase family.

Contains 1 C2 domain.

Contains 1 PI3K/PI4K domain.

Contains 1 PX (phox homology) domain.

CAUTION: It is uncertain whether Met-1 or Met-26 is the initiator.

SEQUENCE CAUTION: The sequence CAA74194.1 differs from that shown. Reason: Erroneous initiation.

Molecular Weight

~184 kDa observed. Weaker cross-reacting bands observed at ~28 and 16 kDa in some lysates.

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in MCF-7 cell lysate. Western Blot Analysis: 2 µg/mL of this antibody detected PI3 Kinase-C2β on 10 µg of MCF-7 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in MCF-7 cell lysate.

Western Blot Analysis: 2 µg/mL of this antibody detected PI3 Kinase-C2β on 10 µg of MCF-7 cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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Anti-PI3 Kinase-C2ß (rabbit polyclonal)

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