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Anti-CAPZIP (rabbit polyclonal)

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货号：07-2189

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB, IP Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number 07-2189
Description Anti-CAPZIP Antibody
Alternate Names
  • RCSD domain containing 1
  • capZ-interacting protein
  • Protein kinase substrate CapZIP
  • protein kinase substrate MK2S4
  • RCSD domain-containing protein 1
Background Information CAPZIP (CapZ-interacting protein) is highly expressed in immune cells and muscle and uniquely interacts with CapZ, an F-actin capping protein, in splenocytes. Phosphorylation of CAPZIP is thought to regulate CapZ ability to remodel actin filament assembly. CAPZIP may also contain a CPI (capping protein interaction) motif that seems to restrict capping protein to a low-affinity, filament-binding conformation.
References
Product Information
Format Affinity Purified
Control
  • Jurkat cell lysate
Presentation Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application Anti-CAPZIP detects levels of CAPZIP proteins & has been published & validated for use in WB & IP.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application Notes Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated CAPZIP from Jurkat cell lysate.
Biological Information
Immunogen GST-tagged recombinant protein corresponding to human CAPZIP.
Host Rabbit
Species Reactivity
  • Human
Species Reactivity Note Demonstrated to react with human. Other homologies: Mouse (64% sequence homology). Rat (58% sequence homology).
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • MK2S4
  • CAPZIP
Purification Method Affinity Purfied
UniProt Number
UniProt Summary FUNCTION: Stress-induced phosphorylation of CAPZIP may regulate the ability of F-actin-capping protein to remodel actin filament assembly.

SUBUNIT STRUCTURE: Interacts with CAPZA2 and CAPZB.

TISSUE SPECIFICITY: Highly expressed in skeletal muscle and more weakly in cardiac muscle. Also expressed in several lymphoid organs, including spleen, thymus, peripheral blood leukocytes, lymph node and bone marrow.

POST-TRANSLATIONAL MODIFICATION: Dephosphorylation results in its dissociation from CAPZA2.

SEQUENCE SIMILARITIES: Contains 1 RCSD domain.

SEQUENCE CAUTION: The sequence CAB70910.1 differs from that shown. Reason: Erroneous initiation.
Molecular Weight ~70 kDa observed. The native form of CAPZIP runs anomalously, with a molecular mass of 70 kDa (much slower than its real molecular mass). The 47 kDa band may be non-specific, a splice variant or a degradation product. (Eyers, C.E., et al. (2005). Biochem J. 389(1):127-135.). An uncharacterized band appears at ~18 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in Jurkat cell lysate.

Western Blot Analysis: 1:250 dilution of this antibody detected CAPZIP on 10 µg of Jurkat cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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