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Anti-phospho-CapZIP (Ser179) (rabbit polyclonal)

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货号：07-2191

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, Ox WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number 07-2191
Description Anti-phospho-CapZIP Antibody (Ser179)
Alternate Names
  • RCSD domain containing 1
  • capZ-interacting protein
  • Protein kinase substrate CapZIP
  • protein kinase substrate MK2S4
  • RCSD domain-containing protein 1
Background Information CapZIP (CapZ-interacting protein) is highly expressed in immune cells and muscle and uniquely interacts with CapZ, an F-actin capping protein, in splenocytes. Phosphorylation of CapZIP is thought to regulate CapZ ability to remodel actin filament assembly. CapZIP may also contain a CPI (capping protein interaction) motif that seems to restrict capping protein to a low-affinity, filament-binding conformation.
References
Product Information
Format Affinity Purified
Control
  • Lambda phosphatase treated and untreated Jurkat plus Sorbitol cell lysate.
Presentation Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application This phospho-CAPZIP antibody is validated for use in WB for the detection of the phospho-CAPZIP protein.
Key Applications
  • Western Blotting
Biological Information
Immunogen KLH-conjugated linear peptide corresponding to human CapZIP phosphorylated at Ser179.
Epitope Phosphorylated Ser179
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity This antibody recognizes CapZIP when phosphorylated at Ser179.
Species Reactivity
  • Human
  • Ox
Species Reactivity Note Demonstrated to react with human. Predicted to react with ox based on 100% sequence homology.
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CAPZIP
  • MK2S4
  • RCSD1
Modifications
  • Phosphorylation
Purification Method Affinity Purfied
UniProt Number
UniProt Summary FUNCTION: Stress-induced phosphorylation of CAPZIP may regulate the ability of F-actin-capping protein to remodel actin filament assembly.

SUBUNIT STRUCTURE: Interacts with CAPZA2 and CAPZB.

TISSUE SPECIFICITY: Highly expressed in skeletal muscle and more weakly in cardiac muscle. Also expressed in several lymphoid organs, including spleen, thymus, peripheral blood leukocytes, lymph node and bone marrow.

POST-TRANSLATIONAL MODIFICATION: Dephosphorylation results in its dissociation from CAPZA2.

SEQUENCE SIMILARITIES: Contains 1 RCSD domain.

SEQUENCE CAUTION: The sequence CAB70910.1 differs from that shown. Reason: Erroneous initiation.
Molecular Weight ~75 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in lambda phosphatase treated and untreated Jurkat plus Sorbitol cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected CapZIP on 10 µg of lambda phosphatase treated and untreated Jurkat plus Sorbitol cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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