Anti-dimethyl-Arginine, asymmetric (ASYM24)

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Anti-dimethyl-Arginine, asymmetric (ASYM24)

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货号：07-414

品牌：Millipore 密理博

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北京朝阳

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EA (预计3-5工作日到货)

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M	WB	Rb	Serum	Polyclonal Antibody

Description
Catalogue Number	07-414
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-dimethyl-Arginine Antibody, asymmetric (ASYM24)
Background Information	Arginine methylation is a post-translational modification that results in the formation of asymmetrical and symmetrical dimethylated arginines (a- and sDMA).

References

Product Information
Format	Serum
Control	Jurkat cell lysate was probed with Anti-dimethyl-Arginine, asymmetric. Several proteins containing asymmetrically dimethylated arginines were detected, including p110, p75 and Sam68. Included Positive Antigen Control: Catalog # 12-303, Jurkat lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.
Presentation	Rabbit antiserum diluted in storage buffer (PBS, 0.035% sodium azide and 30% glycerol). Store at -20°C.

Applications
Application	Detect dimethyl-Arginine with Anti-dimethyl-Arginine Antibody, asymmetric (ASYM24) (Rabbit Polyclonal Antibody), that has been shown to work in WB.
Key Applications	Western Blotting

Biological Information
Immunogen	KLH-conjugated, synthetic peptide containing asymmetric dimethyl-arginine-glycine repeats (KGRGRGRGRGPPPPPRGRGRGRG in which R* denotes asymmetric dimethylation of arginine).
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Recognizes proteins that contain arginines that are asymmetrically dimethylated including p300, p110, p75 and Sam68. Specificity for asymmetrically dimethylated arginine was determined by ELISA with an unmethylated ‘backbone’ peptide or an identical peptide containing symmetrically dimethylated arginines.
Isotype	IgG
Species Reactivity	Human Mouse
Species Reactivity Note	Human and mouse. Reactivity with other species has not been confirmed.
Antibody Type	Polyclonal Antibody
Modifications	Methylation
Purification Method	Unpurified
Molecular Weight	Varies. Antibody detects proteins containing asymmetrical dimethylated arginines.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Routinely evaluated by Western Blot HeLa nuclear extract and p110, p75, and Sam68 in RIPA lysates from Jukat cells. Western Blot Analysis: A 1:500-1:1000 dilution of this lot detected p110, p75, and Sam68 in RIPA lysates from Jurkat cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.