Anti-dimethyl-Histone H4 (Arg3) Symmetric (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	WB, ICC, DB	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

WB, ICC, DB

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	07-947
Description	Anti-dimethyl-Histone H4 (Arg3) Symmetric Antibody
Alternate Names	histone 4, H4 histone H4 histone cluster 4, H4
Background Information	Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications, which include acetylation, phosphorylation, methylation, and ubiquitin-ation, occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Arginine modifications of histones proteins are mediated by two classes of protein arginine methyltransferases (PRMTs), class I and II. Class I PRMTs place two methyl groups on the one nitrogen atom of the arginine guanidino group, resulting in an asymmetric modification. In contrast class II PRMTs place two methyl groups on two nitrogen atoms of the guanidino group producing a symmetric dimethylation. Although there are exceptions, asymmetric modifications are typically associated with transcription activation while symmetric modifications are most commonly associated with transcriptional repression.

Description

Catalogue Number

07-947

Description

Anti-dimethyl-Histone H4 (Arg3) Symmetric Antibody

Alternate Names

histone 4, H4
histone H4
histone cluster 4, H4

Background Information

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications, which include acetylation, phosphorylation, methylation, and ubiquitin-ation, occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Arginine modifications of histones proteins are mediated by two classes of protein arginine methyltransferases (PRMTs), class I and II. Class I PRMTs place two methyl groups on the one nitrogen atom of the arginine guanidino group, resulting in an asymmetric modification. In contrast class II PRMTs place two methyl groups on two nitrogen atoms of the guanidino group producing a symmetric dimethylation. Although there are exceptions, asymmetric modifications are typically associated with transcription activation while symmetric modifications are most commonly associated with transcriptional repression.

Product Information
Format	Affinity Purified
Control	HeLa acid extract
Presentation	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Affinity Purified

Control

HeLa acid extract

Presentation

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	Use Anti-dimethyl-Histone H4 (Arg3) Symmetric Antibody (rabbit polyclonal antibody) validated in WB, ICC, DB to detect dimethyl-Histone H4 (Arg3) Symmetric also known as histone H4, histone cluster 4 H4.
Key Applications	Western Blotting Immunocytochemistry Dot Blot
Application Notes	Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone H4 in HeLa and A431 cells. Peptide Dot Blot Analysis: 0.5 µg/mL of this antibody detected Histone H4 dimethyl Arg3 (symmetric) peptides, but did not detect Histone H4 dimethyl Arg3 (assymmetric) peptide, other modified peptides, or non-methyated histone H4 peptides.

Applications

Application

Use Anti-dimethyl-Histone H4 (Arg3) Symmetric Antibody (rabbit polyclonal antibody) validated in WB, ICC, DB to detect dimethyl-Histone H4 (Arg3) Symmetric also known as histone H4, histone cluster 4 H4.

Key Applications

Western Blotting
Immunocytochemistry
Dot Blot

Application Notes

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone H4 in HeLa and A431 cells.

Peptide Dot Blot Analysis: 0.5 µg/mL of this antibody detected Histone H4 dimethyl Arg3 (symmetric) peptides, but did not detect Histone H4 dimethyl Arg3 (assymmetric) peptide, other modified peptides, or non-methyated histone H4 peptides.

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to human Histone H4 when symmetrically dimethylated at Arg3.
Epitope	Symmetrically dimethylated Arg3
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	This antibody recognizes Histone H4 symmetrically dimethylated at Arg3.
Species Reactivity	Human Mouse Rat
Species Reactivity Note	Demonstrated to react with human, mouse, and rat. Broad species cross-reactivity expected based on sequence similarity.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_001029249
Entrez Gene Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. [provided by RefSeq].
Gene Symbol	HIST1H4A H4/J HIST1H4F HIST1H4L H4FN H4FA H4FH HIST1H4H H4F2 H4FM H4/A H4FK H4FG HIST2H4 H4FB H4/K HIST1H4I H4/N HIST1H4B H4FE H4/M H4/a HIST1H4E HIST4H4 HIST2H4A H4FC H4FI H4/H HIST1H4C H4/G HIST1H4K H4FJ H4FD H4/I H4/B H4/D H4/C HIST1H4J HIST1H4D H4/E
Modifications	Methylation
Purification Method	Affinity Purfied
UniProt Number	P62805
UniProt Summary	FUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. SUBCELLULAR LOCATION: Nucleus. Chromosome. PTM: Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin. Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation. Monomethylation at Arg-4 (H4R3me1) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2a) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage. Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing. Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me). Sumoylated, which is associated with transcriptional repression. SEQUENCE SIMILARITIES: Belongs to the histone H4 family. SEQUENCE CAUTION: The sequence AAI28106.1 differs from that shown. Reason: Frameshift at position 3.
Molecular Weight	~13 kDa observed. Crossreacting band at ~58 kDa has been observed in some lysates.

Biological Information

Immunogen

KLH-conjugated linear peptide corresponding to human Histone H4 when symmetrically dimethylated at Arg3.

Epitope

Symmetrically dimethylated Arg3

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

This antibody recognizes Histone H4 symmetrically dimethylated at Arg3.

Species Reactivity

Human
Mouse
Rat

Species Reactivity Note

Demonstrated to react with human, mouse, and rat. Broad species cross-reactivity expected based on sequence similarity.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_001029249

Entrez Gene Summary

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. [provided by RefSeq].

Gene Symbol

HIST1H4A
H4/J
HIST1H4F
HIST1H4L
H4FN
H4FA
H4FH
HIST1H4H
H4F2
H4FM
H4/A
H4FK
H4FG
HIST2H4
H4FB
H4/K
HIST1H4I
H4/N
HIST1H4B
H4FE
H4/M
H4/a
HIST1H4E
HIST4H4
HIST2H4A
H4FC
H4FI
H4/H
HIST1H4C
H4/G
HIST1H4K
H4FJ
H4FD
H4/I
H4/B
H4/D
H4/C
HIST1H4J
HIST1H4D
H4/E

Modifications

Methylation

Purification Method

Affinity Purfied

UniProt Number

P62805

UniProt Summary

FUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

SUBCELLULAR LOCATION: Nucleus. Chromosome.

PTM: Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.

Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.

Monomethylation at Arg-4 (H4R3me1) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6.

Symmetric dimethylation on Arg-4 (H4R3me2a) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.

Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.

Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).

Sumoylated, which is associated with transcriptional repression.

SEQUENCE SIMILARITIES: Belongs to the histone H4 family.

SEQUENCE CAUTION: The sequence AAI28106.1 differs from that shown. Reason: Frameshift at position 3.

Molecular Weight

~13 kDa observed. Crossreacting band at ~58 kDa has been observed in some lysates.

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in HeLa acid extract. Western Blot Analysis: 0.1 µg/mL of this antibody detected Histone H4 on 10 µg of HeLa acid extract.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in HeLa acid extract.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Histone H4 on 10 µg of HeLa acid extract.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-dimethyl-Histone H4 (Arg3) Symmetric (rabbit polyclonal)

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