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Anti-JHDM1B (rabbit polyclonal)

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货号：09-864

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB, ICC Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number 09-864
Description Anti-JHDM1B Antibody
Alternate Names
  • JMJD
  • lysine (K)-specific demethylase 2B
  • F-box protein FBL10
  • [Histone-H3]-lysine-36 demethylase 1B
  • JEMMA (Jumonji domain, EMSY-interactor, methyltransferase motif) protein
  • F-box and leucine-rich repeat protein 10
  • protein containing CXXC domain 2
  • jumonji C domain-containing histone demethylase 1B
  • Jumonji domain-containing EMSY-interactor methyltransferase motif protein
  • lysine-specific demethylase 2B
  • Protein-containing CXXC domain 2
  • JmjC domain-containing histone demethylation protein 1B
  • Protein JEMMA
  • F-box/LRR-repeat protein 10
  • CXXC-type zinc finger protein 2
Background Information JmjC domain-containing histone demethylation protein 1B, also known as Lysine-specific demethylase 2B, is a nuclear enzyme encoded by the JHDM1B gene (also known as KDM2B) in human. JHDM1A/KDM2B exhibits H3K36-specific demethylase activity with a preference for H3K36me2 over H3K36me and H3K36me3 (PMID 16362057). JHDM1A/KDM2B is also reported to negatively regulate cell proliferation by targeting rDNA and thereby repressing the transcription of rRNA in a JmjC-domain-dependent manner (PMID 17994099).
References
Product Information
Format Affinity Purified
Control
  • HeLa nuclear extract
Presentation Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application Anti-JHDM1B Antibody is a rabbit polyclonal antibody for detection of JHDM1B also known as lysine (K)-specific demethylase 2B, F-box protein FBL10, [Histone-H3]-lysine-36 demethylase 1B & has been validated in WB, ICC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application Notes Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected JHDM1B in NIH/3T3 and HeLa cells.
Immunocytochemistry Analysis: 2 µg/mL from a representative lot detected JHDM1B in A431 cells.
Biological Information
Immunogen KLH-conjugated linear peptide corresponding to human JHDM1B.
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity Note Human, Mouse, Rat. Predicted to react with Rabbit, Chimpanzee, Horse, and Porcine based on 100% sequence homology.
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq].
Gene Symbol
  • KDM2B
  • CXXC2
  • FBXL10
  • JHDM1B
  • PCCX2
  • Fbl10
  • FBL10
Purification Method Affinity Purfied
UniProt Number
Molecular Weight ~165 kDa observed. An uncharacterized lower molecular weight band may be observed in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in HeLa nuclear extract.

Western Blot Analysis: 1 µg/mL of this antibody detected JHDM1B on 10 µg of HeLa nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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