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Anti-ATG12, clone EPR4800 Rabbit Monoclonal

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货号：MABC135

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H ICC, IH(P), WB M Purified Monoclonal Antibody
Description
Catalogue Number MABC1235
Description Anti-N-Myc Interactor Antibody, clone 16E9.2
Alternate Names
  • N-myc-interactor
  • N-myc and STAT interactor
  • Nmi
Background Information N-myc-interactor (UniProt Q13287; also known as N-myc and STAT interactor, Nmi) is encoded by the NMI gene (Gene ID 9111) in human. Nmi is expressed in all adult and fetal tissues except brain and skin and is shown to be more abundant in fetal liver. It interacts with all STATs, except STAT2 and augments STAT-mediated transcription in response to IL-2 and gamma-interferon. Interestingly, Nmi lacks an intrinsic transcriptional activation domain; instead, it enhances the association of CBP/p300 coactivator proteins with STAT1 and STAT5. Higher expression of Nmi has been reported in myeloid leukemia that also express higher levels of c-Myc. Ref.: Zhu, M., et al. (1999). Cell 96, 121-130.
References
Product Information
Format Purified
Presentation Purified mouse IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application Detect N-Myc Interactor using this mouse monoclonal Anti-N-Myc Interactor Antibody, clone 16E9.2, Cat. No. MABC1235, validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application Notes Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected N-Myc Interactor in 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized HeLa and A431 cells.

Immunocytochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected N-Myc Interactor in human cerebellum, small instestine, and tonsil tissue sections.
Biological Information
Immunogen GST-tagged recombinant human N-Myc Interactor N-terminal fragment.
Clone 16E9.2
Concentration Please refer to lot specific datasheet.
Host Mouse
Specificity Clone 16E9.2 targeted epitope resides within the N-terminal half of N-Myc Interactor.
Isotype IgG2aκ
Species Reactivity
  • Human
Species Reactivity Note Human.
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • NMI
Purification Method Protein G purified.
UniProt Number
Molecular Weight ~39 kDa observed. 35.06 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected N-Myc Interactor in 10 µg of K562 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 μg
Transport Information
Supplemental Information
Specifications

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