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Qiagen 凯杰 Biosharp Omega

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Anti-ATG5, clone EPR1755(2) Rabbit Monoclonal

价:
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号:MABC137

牌:Millipore 密理博

账期 货到付款

EA (预计3-5工作日到货)

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Replacement Information

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB, ICC, IHC Rb Serum Polyclonal Antibody
Description
Catalogue Number AB15404
Replaces MABC137
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-APG5 Antibody
Alternate Names
  • Autophagy-related protein 5
  • APG5-like
  • ATG5
  • Apoptosis-specific protein
Background Information Autophagy is the process by which cells recycle cytoplasm and dispose of excess or defective organelles. This process is suggested to be involved development, differentiation, growth regulation and tissue remodeling in multicellular organisms. APG5 conjugates to APG12 and this conjugate is required for elongation of the isolation membrane to form a complete spherical autophagosome. The APG5 protein may play an important role in the apoptotic process, possibly within the modified cytoskeleton.
References
Product Information
Format Serum
Presentation Lyophilized. Reconstitute with 50 μL of sterile distilled water. Centrifuge to remove any insoluble material. Contains no preservative.
Applications
Application This Anti-APG5 Antibody is validated for use in WB, IC, IH for the detection of APG5.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry
Application Notes Western blot: 1:200-1:1,000

Immunocytochemistry: 1:200-1:1,000

Immunohistochemistry: 1:200-1:1,000

Optimal working dilutions must be determined by end user.
Biological Information
Immunogen Synthetic peptide corresponding to a region within the C-terminus of human APG5 protein.
Host Rabbit
Specificity APG5 [Autophagy-related protein 5, APG5-like, ATG5, Apoptosis-specific protein].
Species Reactivity
  • Human
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • ATG5
  • ASP
  • APG5-like
  • APG5
  • OTTHUMP00000040507
  • APG5L
  • hAPG5
Purification Method Serum
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q9H1Y0 # May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
SIZE: 275 amino acids; 32447 Da
SUBCELLULAR LOCATION: Cytoplasm. Note=Colocalizes with nonmuscle actin.
TISSUE SPECIFICITY: Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.
PTM: Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.
SIMILARITY: SwissProt: Q9H1Y0 ## Belongs to the ATG5 family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain lyophilized material frozen at -20°C to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1, ACS or better grade) can be added for additional stability.
Packaging Information
Material Size 50 µL
Transport Information
Supplemental Information
Specifications

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