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Anti-DRAM2 (mouse monoclonal)

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货号：MABC64

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB M Purified Monoclonal Antibody
Description
Catalogue Number MABC64
Description Anti-DRAM2 Antibody
Alternate Names
  • DNA damage-regulated autophagy modulator protein 2
  • Transmembrane protein 77
Background Information Damage-Regulated Autophagy Modulator (DRAM) is a lysosomal protein that acts as an effector molecule that is critical for autophagy and p53-mediated apoptosis. In humans, there are 5 known proteins that show significant homology to DRAM. The closest of these proteins is DRAM2, which displays 67% conservation when compared to DRAM. However, unlike DRAM, DRAM2 is not induced by p53 or p73. Additionally, it does not modulate autophagy in contrast with DRAM. DRAM2 is known to be co-localized with DRAM in lysosomes, and co-expression with DRAM is shown to significantly increase cell death.
References
Product Information
Format Purified
Control
  • Human placenta tissue lysate
Presentation Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application Anti-DRAM2 Antibody detects level of DRAM2 & has been published & validated for use in WB.
Key Applications
  • Western Blotting
Biological Information
Immunogen Linear peptide corresponding to the human DRAM2
Clone 4H6.1
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Isotype IgG2aκ
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • DRAM2
  • TMEM77
Purification Method Protein G Purified
UniProt Number
UniProt Summary FUNCTION: Induces apoptotic cell death when co-expressed with DRAM1.

SUBCELLULAR LOCALIZATION: Lysosome membrane; Multi-pass membrane protein.

TISSUE SPECIFICITY: Expression is down-regulated in ovarian tumors (at protein level). Widely expressed with highest levels in placenta and heart. Not detected in brain or thymus.
Molecular Weight ~29 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in human placenta tissue lysate.

Western Blot Analysis: 1 µg/mL dilution detected DRAM2 in 10 µg of human placenta tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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