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Anti-RALY (rabbit polyclonal)

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货号：AB12226

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB, IP Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB12226
Description Anti-RALY Antibody
Alternate Names
  • RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog (mouse))
  • RNA-binding protein (autoantigenic)
  • hnRNP associated with lethal yellow protein homolog
  • hnRNP core protein C-like 2
  • RNA-binding protein (autoantigenic, hnRNP-associated with lethal yellow)
  • Heterogeneous nuclear ribonucleoprotein C-like 2
  • RNA binding protein (autoantigenic, hnRNP-associated with lethal yellow)
  • Autoantigen p542
Background Information RNA-binding protein RALY is a member of the RRM HNRPC family and contains a characteristic RRM (RNA recognition motif) domain. It is thought to participate in pre-mRNA splicing and may be a heterogenous nuclear ribonucleoprotein. Phosphorylation of RALY has been observed following damage to DNA. Expression has been observed in brain, heart, kidney, liver, lung, pancreas, and skeletal muscle.
References
Product Information
Format Affinity Purified
Control
  • HepG2 cell lysate
Presentation Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application Use Anti-RALY Antibody (rabbit polyclonal antibody) validated in WB, IP to detect RALY also known as hnRNP core protein C-like 2, RNA-binding protein (autoantigenic hnRNP-associated with lethal yellow).
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application Notes Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated RALY from HepG2 cell lysate.
Biological Information
Immunogen Histidine-tagged recombinant protein corresponding to human RALY.
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary In infectious mononucleosis, anti-EBNA-1 antibodies are produced which cross-react with multiple normal human proteins. The cross-reactivity is due to anti-gly/ala antibodies that cross-react with host proteins containing configurations like those in the EBNA-1 repeat. One such antigen is RALY which is a member of the heterogeneous nuclear ribonucleoprotein gene family. [provided by RefSeq].
Gene Symbol
  • P542
  • HNRPCL2
  • RALY
Purification Method Affinity Purfied
UniProt Number
UniProt Summary FUNCTION: Probable-RNA binding protein. Could be a heterogeneous nuclear ribonucleoprotein (hnRNP). May be involved in pre-mRNA splicing (By similarity).

SUBUNIT STRUCTURE: Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8.

SUBCELLULAR LOCATION: Nucleus Probable.

TISSUE SPECIFICTY: Expressed in heart, brain, lung, liver, skeletal muscle, kidney and pancreas. Weakly expressed in placenta.

PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.6 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.19 Ref.20

MISCELLANEOUS: Autoantigen found in infectious mononucleosis caused by Epstein-Barr virus. An epitope recognized by B-cells, which cross-react with the BKRF1 protein (EBNA-1 nuclear protein) of Epstein-Barr virus has been identified.

SEQUENCE SIMILARITIES: Belongs to the RRM HNRPC family. RALY subfamily.

Contains 1 RRM (RNA recognition motif) domain.
Molecular Weight ~ 38 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in HepG2 cell lysate.

Western Blot Analysis: 1:10,000 dilution of this antibody detected RALY on 10 µg of HepG2 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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