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Anti-STAT1 (Mouse Monoclonal IgG)

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货号：04-069

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB, ICC, IHC M Purified Monoclonal Antibody
Description
Catalogue Number MABE269
Replaces 04-1069
Description Anti-Musashi-2 Antibody
Alternate Names
  • RNA-binding protein Musashi homolog 2
  • Musashi-2
Background Information Musashi-2 (Musashi homolog 2; Msi2) is a member of the Musashi family of RNA-binding proteins that binds to mRNA sequences via their RNA-binding motifs. It is highly expressed in mammalian neural precursor cells and is involved in the post-transcriptional regulation of genes affecting CNS development. Musashi-2 is also expressed in subpopulations of neural cells in the mature CNS. Previous studies have demonstrated that the expression of Musashi-2 increases several days after brain injury. Other studies have suggested that Musashi-2 may also play a role in the pathogenesis of myeloid leukemia.
References
Product Information
Format Purified
Control
  • PC-12 cell lysate
Presentation Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application Anti-Musashi-2 Antibody is an antibody against Musashi-2 for use in Western Blotting, ICC & IHC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry
Application Notes Western Blot Analysis: 1 µg/mL from a representative lot detected Musashi-2 in 10 µg of T47D cell lysate.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Musashi-2 in HeLa cells.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Musashi-2 in neurons and glial cells of rat pons tissues and in neurons and astrocytes of rat frontal cortex tissues.
Biological Information
Immunogen GST tagged recombinant protein corresponding to human Musashi-2.
Clone 6E7.2
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Isotype IgG1κ
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Two transcript variants encoding distinct isoforms have been identified for this gene.
Gene Symbol
  • MSI2
Purification Method Protein G Purified
UniProt Number
UniProt Summary FUNCTION: RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system.

SUBCELLULAR LOCATION: Cytoplasm. Note: Associated with polysomes.

TISSUE SPECIFICITY: Ubiquitous; detected at low levels.

INDUCTION: Up-regulated in astrocytes after brain injury.

POST-TRANSLATIONAL MODIFICATION: Phosphorylated.

INVOLVEMENT IN DISEASE: Note=Chromosomal aberrations involving MSI2 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with HOXA9; translocation t(7;17)(q32-34;q23).

SEQUENCE SIMILARITIES: Belongs to the Musashi family. Contains 2 RRM (RNA recognition motif) domains.
Molecular Weight ~38 kDa observed. Uniprot describes two other isoforms at ~28 kDa and ~17 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in PC-12 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Musashi-2 in 10 µg of PC-12 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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