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Anti-Raptor (rabbit polyclonal)

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货号：09-217

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H IP, WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number 09-217
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-Raptor Antibody
Alternate Names
  • p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats, regulatory associated protein of mTOR
References
Product Information
Format Affinity Purified
Presentation Affinity purified rabbit polyclonal IgG in 0.02M PBS, 0.25M sodium chloride containing 0.1% sodium azide and 50% glycerol.
Applications
Application Detect Raptor using this anti-Raptor Antibody validated for use in IP & WB.
Key Applications
  • Immunoprecipitation
  • Western Blotting
Biological Information
Immunogen KLH conjugated peptide encompassing the N-terminal region of human Raptor.
Host Rabbit
Isotype IgG
Species Reactivity
  • Human
Species Reactivity Note Other species have not been tested.
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • KIAA1303
  • Raptor
Purification Method ImmunoAffinity Purified
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q8N122 # Participates in the FRAP1 pathway and associates in a near stoichiometric ratio with FRAP1 to form a nutrient-sensitive complex (NSC). Plays a pivotal role as a scaffold protein in the FRAP1-signaling pathway and this interaction is essential for the catalyzed phosphorylation of EIF4EBP1. Has a positive role in nutrient-stimulated signaling to the downstream effector RPS6KB1. Under nutrient-deprived conditions, serves as a negative regulator of FRAP1 kinase activity. Regulation of the interaction with FRAP1 is a critical mechanism by which cells coordinate the rate of cell growth and maintenance of cell size with different environmental conditions.
SIZE: 1335 amino acids; 149038 Da
SUBUNIT: Binds directly 4EBP1 and RPS6KB1 independently of its association with FRAP1. Binds preferentially to poorly or non- phosphorylated form of EIF4EBP1, and this binding is critical to the ability of FRAP1 to catalyze phosphorylation. Complex with FRAP1 physically interacts under both leucine-rich and -poor conditions and therefore in at least two nutrient-determined states with different stability.
TISSUE SPECIFICITY: Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta.
SIMILARITY: SwissProt: Q8N122 ## Contains 7 WD repeats.
MISCELLANEOUS: Rapamycin destabilizes the interaction with FRAP1 regardless of nutrient availability, and its potency for dissociation is increased under nutrient-rich conditions. This action uncouples FRAP1 from its substrates, and inhibits FRAP1 signaling without altering its intrinsic catalytic activity.
Molecular Weight 150 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Routinely evaluated by immunoblot.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions 2 years at -20°C from date of shipment
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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