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Anti-PP5 (rabbit polyclonal)

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货号：07-1225

品牌：Millipore 密理博

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Replacement Information
Replacement Information 07-1225 is a recommended replacement for AB4092

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M WB, IH(P) Rb Purified Polyclonal Antibody
Description
Catalogue Number 07-1225
Replaces AB4092
Trade Name
  • Upstate
Description Anti-PP5 Antibody
Alternate Names
  • Protein phosphatase T
  • Serine/Threonine phosphatase
  • Serine/threonine-protein phosphatase 5
  • Protein phosphatase 5, catalytic subunit
Background Information Protein phosphatase 5 (PP5) is a novel human protein serine/threonine phosphatase of molecular weight of 58 kDa. It is made up of a C-terminal phosphatase catalytic domain and an N-terminal domain, which has four repeats of 34 amino acids, three of which are tandemly arranged. The phosphatase domain contains all of the invariant motifs of the PP1/PP2A/PP2B gene family. However, it is not closely related to any other known member of this family of phosphatases. Thus it makes up a new subfamily of phosphatases. PP5 expressed in bacteria has been shown to dephosphorylate serine residues in proteins and is more sensitive than PP1 to the tumor promoter okadaic acid. PP5 appears to be localized to the nucleus where it is thought to play a roll in the regulation of RNA biogenesis and mitosis as part of a signaling pathway regulating cell cycle progression.
References
Product Information
Format Purified
Control
  • Jurkat Cell Lysate, A549 and MCF-7 cell lysates.
Presentation Purified rabbit polyclonal IgG in phosphate buffered saline with 0.08% sodium azide
Applications
Application Detect PP5 using this Anti-PP5 Antibody validated for use in WB, IH(P).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application Notes Immunohistochemistry(paraffin):
Representative images from a previous lot.

Optimal Staining of PP5 Polyclonal Antibody: Human Testis
Biological Information
Immunogen Synthetic peptide derived from the human protein phosphatase 5 protein.
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Recognizes Protein Phosphatase 5 (PP5).
Isotype IgG
Species Reactivity
  • Human
  • Mouse
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • PPP5C
  • PPP5
  • FLJ36922
  • PP-T
  • PP5
  • PPT
  • EC 3.1.3.16
UniProt Number
UniProt Summary FUNCTION: SwissProt: P53041 # May play a role in the regulation of RNA biogenesis and/or mitosis. In vitro, dephosphorylates serine residues of skeletal muscle phosphorylase and histone H1.
COFACTOR: Binds 1 iron ion per subunit (By similarity). & Binds 1 manganese ion per subunit (By similarity).
SIZE: 499 amino acids; 56879 Da
SUBUNIT: Interacts with CDC16 and CDC27.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Predominantly nuclear. But also present in the cytoplasm.
TISSUE SPECIFICITY: Ubiquitous.
SIMILARITY: SwissProt: P53041 ## Belongs to the PPP phosphatase family. PP-5 (PP-T) subfamily. & Contains 3 TPR repeats.
Molecular Weight 58 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Routinely evaluated by Western Blot on Jurkat lysates.

Western Blot Analysis: 1:500 dilution of this lot detected PP-5 on 10 μg of Jurkat lysates
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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