Anti-SHIP (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, IP	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

WB, IP

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	ABS10
Description	Anti-SHIP Antibody
Alternate Names	inositol polyphosphate-5-phosphatase, 145kDa SH2 containing inositol phosphatase signaling inositol polyphosphate 5 phosphatase SIP-145 Inositol polyphosphate-5-phosphatase of 145 kDa inositol polyphosphate-5-phosphatase, 145kD SH2 domain-containing inositol phosphatase 1 SH2 containing inositol phosphatase, isoform b SH2 domain-containing inositol-5'-phosphatase 1
Background Information	Expressed only in hematopoietic cells, Src homology (SH)-2 containing Inositol phosphatase (SHIP) is a member of the Inositol polyphosphatase-5-phosphatase (INPP5) family characterized as containing several protein interaction domains that include an Inositol phosphatase domain, a SH2 domain, and a proline-rich domain. SHIP hydrolyzes the phosphatidylinositol 3-kinase product phosphatidylinositol-3,4,5-triphosphatase, a requirement of activation for several enzymes containing pleckstrin homology domains. The movement of this protein from cytosol to the plasma membrane is regulated by tyrosine phosphorylation as a response to various activations by cytokines and T and B cell receptors. SHIP is a necessary negative regulator, moderating the balance of both the positive and negative signals in myeloid cells and lymphocytes. There are multiple forms of SHIP, which have been shown to result from the truncation of the C-terminus.

Description

Catalogue Number

ABS10

Description

Anti-SHIP Antibody

Alternate Names

inositol polyphosphate-5-phosphatase, 145kDa
SH2 containing inositol phosphatase
signaling inositol polyphosphate 5 phosphatase SIP-145
Inositol polyphosphate-5-phosphatase of 145 kDa
inositol polyphosphate-5-phosphatase, 145kD
SH2 domain-containing inositol phosphatase 1
SH2 containing inositol phosphatase, isoform b
SH2 domain-containing inositol-5'-phosphatase 1

Background Information

Expressed only in hematopoietic cells, Src homology (SH)-2 containing Inositol phosphatase (SHIP) is a member of the Inositol polyphosphatase-5-phosphatase (INPP5) family characterized as containing several protein interaction domains that include an Inositol phosphatase domain, a SH2 domain, and a proline-rich domain. SHIP hydrolyzes the phosphatidylinositol 3-kinase product phosphatidylinositol-3,4,5-triphosphatase, a requirement of activation for several enzymes containing pleckstrin homology domains. The movement of this protein from cytosol to the plasma membrane is regulated by tyrosine phosphorylation as a response to various activations by cytokines and T and B cell receptors. SHIP is a necessary negative regulator, moderating the balance of both the positive and negative signals in myeloid cells and lymphocytes. There are multiple forms of SHIP, which have been shown to result from the truncation of the C-terminus.

Product Information
Format	Affinity Purified
Control	TF-1 cell lysate
Presentation	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Affinity Purified

Control

TF-1 cell lysate

Presentation

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	Anti-SHIP Antibody detects level of SHIP & has been published & validated for use in WB & IP.
Key Applications	Western Blotting Immunoprecipitation
Application Notes	Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated SHIP protein from Ramos lysate.

Applications

Application

Anti-SHIP Antibody detects level of SHIP & has been published & validated for use in WB & IP.

Key Applications

Western Blotting
Immunoprecipitation

Application Notes

Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated SHIP protein from Ramos lysate.

Biological Information
Immunogen	GST-tagged recombinant protein corresponding to the C-terminus of human SHIP.
Epitope	C-terminus
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	This antibody recognizes SHIP at the C-terminus.
Species Reactivity	Human
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_001017915
Entrez Gene Summary	This gene is a member of the inositol polyphosphate-5-phosphatase (INPP5) family and encodes a protein with an N-terminal SH2 domain, an inositol phosphatase domain, and two C-terminal protein interaction domains. Expression of this protein is restricted to hematopoietic cells where its movement from the cytosol to the plasma membrane is mediated by tyrosine phosphorylation. At the plasma membrane, the protein hydrolyzes the 5' phosphate from phosphatidylinositol (3,4,5)-trisphosphate and inositol-1, 3, 4, 5-tetrakisphosphate, thereby affecting multiple signaling pathways. Overall, the protein functions as a negative regulator of myeloid cell proliferation and survival. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].
Gene Symbol	INPP5D hp51CN p150Ship SHIP SIP-145 SHIP-1 SHIP1
Purification Method	Affinity Purfied
UniProt Number	Q92835
UniProt Summary	FUNCTION: Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity. Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression. May also hydrolyze PtdIns(1,3,4,5)P4, and could thus affect the levels of the higher inositol polyphosphates like InsP6. CATALYTIC ACTIVITY: Phosphatidylinositol 3,4,5-trisphosphate + H2O = phosphatidylinositol 3,4-bisphosphate + phosphate. ENZYME REGULATION: Activated upon translocation to the sites of synthesis of PtdIns(3,4,5)P3 in the membrane (By similarity). SUBUNIT STRUCTURE: Interacts with tyrosine phosphorylated forms of SHC1, DOK1, DOK3, PTPN11/SHP-2, SLAMF1/CD150. Interacts with PTPN11 in response to IL-3. Interacts with receptors EPOR, MS4A2/FCER1B and FCER1G, FCGR2A, FCGR2B and FCGR3. Interacts with GRB2 and PLCG1. Interacts with tyrosine kinases SRC and TEC. Interacts with FCGR2A, leading to regulate gene expression during the phagocytic process. Interacts with c-Met/MET (By similarity). SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein. Note: Translocates to the plasma membrane when activated, translocation is probably due to different mechanisms depending on the stimulus and cell type. Partly translocated via its SH2 domain which mediates interaction with tyrosine phosphorylated receptors such as the FC-gamma-RIIB receptor (FCGR2B) or CD16/FCGR3. Tyrosine phosphorylation may also participate to membrane localization (By similarity). TISSUE SPECIFICITY: Specifically expressed in immune and hematopoietic cells. Expressed in bone marrow and blood cells. Levels vary considerably within this compartment. Present in at least 74% of immature CD34+ cells, whereas within the more mature population of CD33+ cells, it is present in only 10% of cells. Present in the majority of T-cells, while it is present in a minority of B-cells (at protein level). DOMAIN: The SH2 domain interacts with tyrosine phosphorylated forms of proteins such as SHC1 or PTPN11/SHP-2. It competes with that of GRB2 for binding to phosphorylated SHC1 to inhibit the Ras pathway. It is also required for tyrosine phosphorylation (By similarity). The NPXY sequence motif found in many tyrosine-phosphorylated proteins is required for the specific binding of the PID domain (By similarity). PTM: Tyrosine phosphorylated by the members of the SRC family after exposure to a diverse array of extracellular stimuli such as cytokines, growth factors, antibodies, chemokines, integrin ligands and hypertonic and oxidative stress. Phosphorylated upon IgG receptor FCGR2B-binding. SEQUENCE SIMILARITIES: Belongs to the inositol-1,4,5-trisphosphate 5-phosphatase family. Contains 1 SH2 domain.
Molecular Weight	~145 kDa observed

Biological Information

Immunogen

GST-tagged recombinant protein corresponding to the C-terminus of human SHIP.

Epitope

C-terminus

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

This antibody recognizes SHIP at the C-terminus.

Species Reactivity

Human

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_001017915

Entrez Gene Summary

This gene is a member of the inositol polyphosphate-5-phosphatase (INPP5) family and encodes a protein with an N-terminal SH2 domain, an inositol phosphatase domain, and two C-terminal protein interaction domains. Expression of this protein is restricted to hematopoietic cells where its movement from the cytosol to the plasma membrane is mediated by tyrosine phosphorylation. At the plasma membrane, the protein hydrolyzes the 5' phosphate from phosphatidylinositol (3,4,5)-trisphosphate and inositol-1, 3, 4, 5-tetrakisphosphate, thereby affecting multiple signaling pathways. Overall, the protein functions as a negative regulator of myeloid cell proliferation and survival. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].

Gene Symbol

INPP5D
hp51CN
p150Ship
SHIP
SIP-145
SHIP-1
SHIP1

Purification Method

Affinity Purfied

UniProt Number

Q92835

UniProt Summary

FUNCTION: Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity. Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression. May also hydrolyze PtdIns(1,3,4,5)P4, and could thus affect the levels of the higher inositol polyphosphates like InsP6.

CATALYTIC ACTIVITY: Phosphatidylinositol 3,4,5-trisphosphate + H2O = phosphatidylinositol 3,4-bisphosphate + phosphate.

ENZYME REGULATION: Activated upon translocation to the sites of synthesis of PtdIns(3,4,5)P3 in the membrane (By similarity).

SUBUNIT STRUCTURE: Interacts with tyrosine phosphorylated forms of SHC1, DOK1, DOK3, PTPN11/SHP-2, SLAMF1/CD150. Interacts with PTPN11 in response to IL-3. Interacts with receptors EPOR, MS4A2/FCER1B and FCER1G, FCGR2A, FCGR2B and FCGR3. Interacts with GRB2 and PLCG1. Interacts with tyrosine kinases SRC and TEC. Interacts with FCGR2A, leading to regulate gene expression during the phagocytic process. Interacts with c-Met/MET (By similarity).

SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein. Note: Translocates to the plasma membrane when activated, translocation is probably due to different mechanisms depending on the stimulus and cell type. Partly translocated via its SH2 domain which mediates interaction with tyrosine phosphorylated receptors such as the FC-gamma-RIIB receptor (FCGR2B) or CD16/FCGR3. Tyrosine phosphorylation may also participate to membrane localization (By similarity).

TISSUE SPECIFICITY: Specifically expressed in immune and hematopoietic cells. Expressed in bone marrow and blood cells. Levels vary considerably within this compartment. Present in at least 74% of immature CD34+ cells, whereas within the more mature population of CD33+ cells, it is present in only 10% of cells. Present in the majority of T-cells, while it is present in a minority of B-cells (at protein level).

DOMAIN: The SH2 domain interacts with tyrosine phosphorylated forms of proteins such as SHC1 or PTPN11/SHP-2. It competes with that of GRB2 for binding to phosphorylated SHC1 to inhibit the Ras pathway. It is also required for tyrosine phosphorylation (By similarity).

The NPXY sequence motif found in many tyrosine-phosphorylated proteins is required for the specific binding of the PID domain (By similarity).

PTM: Tyrosine phosphorylated by the members of the SRC family after exposure to a diverse array of extracellular stimuli such as cytokines, growth factors, antibodies, chemokines, integrin ligands and hypertonic and oxidative stress. Phosphorylated upon IgG receptor FCGR2B-binding.

SEQUENCE SIMILARITIES: Belongs to the inositol-1,4,5-trisphosphate 5-phosphatase family.

Contains 1 SH2 domain.

Molecular Weight

~145 kDa observed

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in TF-1 cell lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected SHIP on 10 µg of TF-1 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in TF-1 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected SHIP on 10 µg of TF-1 cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-SHIP (rabbit polyclonal)

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