Anti-erbB-3/HER-3, clone 2F12

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, R, M, B	ICC, IHC, IP, WB	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, R, M, B

ICC, IHC, IP, WB

Purified

Monoclonal Antibody

Description
Catalogue Number	05-390
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-erbB-3/HER-3 Antibody, clone 2F12
Alternate Names	Tyrosine kinase-type cell surface receptor HER3 lethal congenital contracture syndrome 2 v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3 v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)
Background Information	The ErbB3 gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. ErbB3 is a membrane-bound protein which has a neuregulin binding domain but not an active kinase domain. It can therefore bind this ligand but cannot convey a signal into the cell via protein phosphorylation. However it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers including prostate, bladder and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported but they have not been thoroughly characterized.

Description

Catalogue Number

05-390

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-erbB-3/HER-3 Antibody, clone 2F12

Alternate Names

Tyrosine kinase-type cell surface receptor HER3
lethal congenital contracture syndrome 2
v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3
v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)

Background Information

The ErbB3 gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. ErbB3 is a membrane-bound protein which has a neuregulin binding domain but not an active kinase domain. It can therefore bind this ligand but cannot convey a signal into the cell via protein phosphorylation. However it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers including prostate, bladder and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported but they have not been thoroughly characterized.

Product Information
Format	Purified
Control	Human breast adenocarcinoma (well-differentiated), mouse brain lysate, MCF-7 cell lysate.
Presentation	Purified mouse monoclonal IgG2aκ in buffer containing 10 mM PBS, pH 7.4, 0.2% BSA, with 0.09% sodium azide. Frozen solution.

Product Information

Format

Purified

Control

Human breast adenocarcinoma (well-differentiated), mouse brain lysate, MCF-7 cell lysate.

Presentation

Purified mouse monoclonal IgG2aκ in buffer containing 10 mM PBS, pH 7.4, 0.2% BSA, with 0.09% sodium azide. Frozen solution.

Applications
Application	Detect erbB-3/HER-3 using this Anti-erbB-3/HER-3 Antibody, clone 2F12 validated for use in IC, IH, IP & WB.
Key Applications	Immunocytochemistry Immunohistochemistry Immunoprecipitation Western Blotting
Application Notes	Immunoprecipitation: 4 µg of a previous lot of this antibody immunoprecipitated erbB-3/HER-3 from human MDA and MCF-7 RIPA lysates. Immunocytochemistry: 10 µg/mL of a previous lot of this antibody gave positive immunostaining for erbB-3/HER-3 in MCF-7cells fixed with 95% ethanol/5% acetic acid. Immunohistochemistry: 1:50 and 1:100 dilution of a previous lot detected erbB-3/HER-3 membrane staining in human normal and carcinoma breast tissue.

Applications

Application

Detect erbB-3/HER-3 using this Anti-erbB-3/HER-3 Antibody, clone 2F12 validated for use in IC, IH, IP & WB.

Key Applications

Immunocytochemistry
Immunohistochemistry
Immunoprecipitation
Western Blotting

Application Notes

Immunoprecipitation:
4 µg of a previous lot of this antibody immunoprecipitated erbB-3/HER-3 from human MDA and MCF-7 RIPA lysates.

Immunocytochemistry:
10 µg/mL of a previous lot of this antibody gave positive immunostaining for erbB-3/HER-3 in MCF-7cells fixed with 95% ethanol/5% acetic acid.

Immunohistochemistry:
1:50 and 1:100 dilution of a previous lot detected erbB-3/HER-3 membrane staining in human normal and carcinoma breast tissue.

Biological Information
Immunogen	Recombinant rat erbB-3/HER-3 oncoprotein expressed in insect cells and purified by metal chelating chromatography. The epitope maps between amino acid residues 1295-1323 of human erbB-3. Clone 2F12.
Epitope	a.a. 1295-1323 of human erbB-3
Clone	2F12
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody recognizes erbB-3/HER-3, Mr 185 kDa. Does not cross-react with erbB-1 (EGFR) and erbB-2/HER-2.
Isotype	IgG2aκ
Species Reactivity	Human Rat Mouse Bovine
Species Reactivity Note	Human. Predicted to cross-react with mouse, rat, and cow.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_001982
Entrez Gene Summary	This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized.
Gene Symbol	ERBB3 c-erbB-3 c-erbB3 erbB-3 p180-ErbB3 p85-sErbB3 p45-sErbB3 ErbB-3 erbB3-S MGC88033 HER3 MDA-BF-1
Purification Method	Protein A chromatography
UniProt Number	P21860
UniProt Summary	FUNCTION: SwissProt: P21860 # Binds and is activated by neuregulins and NTAK. SIZE: 1342 amino acids; 148098 Da SUBUNIT: Heterodimer with each of the other ERBB receptors (Potential). Interacts with CSPG5 and PA2G4. SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted. TISSUE SPECIFICITY: Epithelial tissues and brain. DOMAIN: SwissProt: P21860 The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins. PTM: Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (By similarity). DISEASE: SwissProt: P21860 # Overexpressed in a subset of human mammary tumors. & Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology. SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
Molecular Weight	185 kDa

Biological Information

Immunogen

Recombinant rat erbB-3/HER-3 oncoprotein expressed in insect cells and purified by metal chelating chromatography. The epitope maps between amino acid residues 1295-1323 of human erbB-3. Clone 2F12.

Epitope

a.a. 1295-1323 of human erbB-3

Clone

2F12

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

This antibody recognizes erbB-3/HER-3, Mr 185 kDa. Does not cross-react with erbB-1 (EGFR) and erbB-2/HER-2.

Isotype

IgG2aκ

Species Reactivity

Human
Rat
Mouse
Bovine

Species Reactivity Note

Human. Predicted to cross-react with mouse, rat, and cow.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_001982

Entrez Gene Summary

This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized.

Gene Symbol

ERBB3
c-erbB-3
c-erbB3
erbB-3
p180-ErbB3
p85-sErbB3
p45-sErbB3
ErbB-3
erbB3-S
MGC88033
HER3
MDA-BF-1

Purification Method

Protein A chromatography

UniProt Number

P21860

UniProt Summary

FUNCTION: SwissProt: P21860 # Binds and is activated by neuregulins and NTAK.
SIZE: 1342 amino acids; 148098 Da
SUBUNIT: Heterodimer with each of the other ERBB receptors (Potential). Interacts with CSPG5 and PA2G4.
SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
TISSUE SPECIFICITY: Epithelial tissues and brain.
DOMAIN: SwissProt: P21860 The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins.
PTM: Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (By similarity).
DISEASE: SwissProt: P21860 # Overexpressed in a subset of human mammary tumors. & Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.

Molecular Weight

185 kDa

Product Usage Statements
Quality Assurance	Routinely evaluated by Western Blot on RIPA lysates of human MCF-7 cells. Western Blot Analysis: 0.1-2 µg/mL of this lot detected erbB-3/HER-3 in RIPA lysates of human MCF-7 cells. Previous lots have detected erbB-3/HER-3 in MDA RIPA lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated by Western Blot on RIPA lysates of human MCF-7 cells.

Western Blot Analysis:
0.1-2 µg/mL of this lot detected erbB-3/HER-3 in RIPA lysates of human MCF-7 cells. Previous lots have detected erbB-3/HER-3 in MDA RIPA lysates.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	200 µg

Packaging Information

Material Size

200 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-erbB-3/HER-3, clone 2F12

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