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Anti-Tyrosinase, clone T311 (mouse monoclonal)

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货号：05-647

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB, IHC M Purified Monoclonal Antibody
Description
Catalogue Number 05-647
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-Tyrosinase Antibody, clone T311
References
Product Information
Format Purified
Presentation 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Applications
Application This Anti-Tyrosinase Antibody, clone T311 is validated for use in WB, IH for the detection of Tyrosinase.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application Notes Not recommended for Immunoprecipitation
Biological Information
Immunogen Purified recombinant Tyrosinase corresponding to residues 5-456 of human Tyrosinase
Clone T311
Host Mouse
Specificity Tyrosinase
Isotype IgG2a
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • TYR
  • LB24-AB
  • tyrosinase
  • OCAIA
  • SK29-AB
  • OCA1A
Purification Method Protein G Chromatography
UniProt Number
UniProt Summary FUNCTION: SwissProt: P14679 # This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone.
COFACTOR: Binds 2 copper ions per subunit.
SIZE: 529 amino acids; 60393 Da
SUBCELLULAR LOCATION: Melanosome membrane; Single-pass type I membrane protein.
DISEASE: SwissProt: P14679 # Defects in TYR are the cause of oculocutaneous albinism type IA (OCA-IA) [MIM:203100]. OCA-I, also known as tyrosinase negative oculocutaneous albinism, is an autosomal recessive disorder characterized by absence of pigment in hair, skin and eyes. OCA-I is divided into 2 types: type IA, characterized by complete lack of tyrosinase activity due to production of an inactive enzyme, and type IB characterized by reduced activity of tyrosinase. OCA-IA patients presents with the life-long absence of melanin pigment after birth and manifest increased sensitivity to ultraviolet radiation and to predisposition to skin cancer. & Defects in TYR are the cause of oculocutaneous albinism type IB (OCA-IB) [MIM:606952]; also known as albinism yellow mutant type. OCA-IB patients have white hair at birth that rapidly turns yellow or blond. They manifest the development of minimal- to-moderate amounts of cutaneous and ocular pigment. & Defects in TYR are the cause of oculocutaneous albinism type I temperature-sensitive (OCA-ITS) [MIM:606952]. OCA-ITS patients have white axillary and scalp hair and pigmented arm and leg hair.
SIMILARITY: SwissProt: P14679 ## Belongs to the tyrosinase family.
Molecular Weight 70-80kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance routinely evaluated by immunoblot on RIPA lysates from SK-MEL-19 cells
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions 2 years at -20°C
Packaging Information
Material Size 200 µg
Transport Information
Supplemental Information
Specifications

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