Anti-PDE4D (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
R, H, Chp	WB	Rb	Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

R, H, Chp

Purified

Polyclonal Antibody

Description
Catalogue Number	ABS22
Description	Anti-PDE4D Antibody
Alternate Names	phosphodiesterase 4D, cAMP-specific cAMP-specific phosphodiesterase PDE4D6 cAMP-specific 3',5'-cyclic phosphodiesterase 4D phosphodiesterase 4D, cAMP-specific (dunce (Drosophila)-homolog phosphodiesterase E3) phosphodiesterase 4D, cAMP-specific (dunce) phosphodiesterase E3 dunce homolog phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila) dunce-like phosphodiesterase E3 cAMP-specific phosphodiesterase 4D
Background Information	Phosphodiesterase 4D (PDE4D) is a member of the PDE cyclic nucleotides. PDE4D is known to be regulated by protein kinase C (PKC)-Raf-MEK-ERK and also by cAMP in vascular smooth muscle cells. PDE4D is essential to have equilibrium in relaxing and contracting cues to airway smooth muscle. Down-regulation of PDE4D may be associated with analgesic and anti-inflammatory effects. It has also been linked to the risk of stroke, specifically common polygenic stroke.

Description

Catalogue Number

ABS22

Description

Anti-PDE4D Antibody

Alternate Names

phosphodiesterase 4D, cAMP-specific
cAMP-specific phosphodiesterase PDE4D6
cAMP-specific 3',5'-cyclic phosphodiesterase 4D
phosphodiesterase 4D, cAMP-specific (dunce (Drosophila)-homolog phosphodiesterase E3)
phosphodiesterase 4D, cAMP-specific (dunce)
phosphodiesterase E3 dunce homolog
phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila)
dunce-like phosphodiesterase E3
cAMP-specific phosphodiesterase 4D

Background Information

Phosphodiesterase 4D (PDE4D) is a member of the PDE cyclic nucleotides. PDE4D is known to be regulated by protein kinase C (PKC)-Raf-MEK-ERK and also by cAMP in vascular smooth muscle cells. PDE4D is essential to have equilibrium in relaxing and contracting cues to airway smooth muscle. Down-regulation of PDE4D may be associated with analgesic and anti-inflammatory effects. It has also been linked to the risk of stroke, specifically common polygenic stroke.

Product Information
Format	Purified
Control	Rat brain tissue lysate
Presentation	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Purified

Control

Rat brain tissue lysate

Presentation

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	Anti-PDE4D Antibody is an antibody against PDE4D for use in WB.
Key Applications	Western Blotting

Applications

Application

Anti-PDE4D Antibody is an antibody against PDE4D for use in WB.

Key Applications

Western Blotting

Biological Information
Immunogen	GST-tagged recombinant protein corresponding to the C-terminus of human PDE4D.
Epitope	C-terminus
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	This antibody recognizes PDE4D at the C-terminus.
Species Reactivity	Rat Human Chimpanzee
Species Reactivity Note	Demonstrated to react with Human and Rat. Predicted to react with Chimpanzee based on 100% sequence homology. Expected to react with Mouse based on 90% sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_001098101
Entrez Gene Summary	This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.
Gene Symbol	PDE4DN2 DPDE3 PDE43 PDE4D STRK1 HSPDE4D
Purification Method	Protein A
UniProt Number	Q08499
UniProt Summary	FUNCTION: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. CATALYTIC ACTIVITY: Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate. COFACTOR: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions. Ref.26 Ref.27 ENZYME REGULATION: Inhibited by rolipram. Activated by phosphatidic acid. PATHWAY: Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1. SUBUNIT STRUCTURE: Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 2 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP (By similarity). Isoform 6, isoform 7 and isoform 12 bind GNB2L1 via their unique N-terminus. Binds ARRB2. SUBCELLULAR LOCATION: Cytoplasm (By similarity). Membrane (By similarity). Cytoplasm › cytoskeleton (By similarity). Cytoplasm › cytoskeleton › centrosome (By similarity). Note: Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome (By similarity). TISSUE SPECIFICTY: Widespread; most abundant in skeletal muscle. Isoform 8 is detected in brain. Isoform 9 is detected in brain, placenta, lung and kidney. Isoform 11 is detected in heart and skeletal muscle. PTM: Isoform 2 and isoform 11 are activated by phosphorylation (in vitro), but not isoform 8. Isoform 7 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59. INVOLVEMENT IN DISEASE: Note=Genetic variations in PDE4D might be associated with susceptibility to stroke. Ref.16 states that association with stroke has to be considered with caution. SEQUENCE SIMLARITIES: Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
Molecular Weight	~ 59, 68, 85, 90, and 105 kDa observed. UniProtKB/Swiss-Prot Q08499 (PDE4D_HUMAN) entry describes 12 isoforms produced by alternative splicing: Isoform 1 at 91.115 kDa, Isoform 2 at 76.369 kDa, Isoform 3 at 68.607 kDa, Isoform 4 at 66.376 kDa, Isoform 5 at 57.792 kDa, Isoform 6 at 84.428 kDa, Isoform 7 at 23.839 kDa, Isoform 8 at 59.113 kDa, Isoform 9 at 77.705 kDa, Isoform 10 at 76.816 kDa, Isoform 11 at 84.662 kDa, and Isoform 12 at 24.429 kDa.

Biological Information

Immunogen

GST-tagged recombinant protein corresponding to the C-terminus of human PDE4D.

Epitope

C-terminus

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

This antibody recognizes PDE4D at the C-terminus.

Species Reactivity

Rat
Human
Chimpanzee

Species Reactivity Note

Demonstrated to react with Human and Rat. Predicted to react with Chimpanzee based on 100% sequence homology.
Expected to react with Mouse based on 90% sequence homology.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_001098101

Entrez Gene Summary

This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.

Gene Symbol

PDE4DN2
DPDE3
PDE43
PDE4D
STRK1
HSPDE4D

Purification Method

Protein A

UniProt Number

Q08499

UniProt Summary

FUNCTION: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

CATALYTIC ACTIVITY: Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.

COFACTOR: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions. Ref.26 Ref.27

ENZYME REGULATION: Inhibited by rolipram. Activated by phosphatidic acid.

PATHWAY: Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.

SUBUNIT STRUCTURE: Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 2 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP (By similarity). Isoform 6, isoform 7 and isoform 12 bind GNB2L1 via their unique N-terminus. Binds ARRB2.

SUBCELLULAR LOCATION: Cytoplasm (By similarity). Membrane (By similarity). Cytoplasm › cytoskeleton (By similarity). Cytoplasm › cytoskeleton › centrosome (By similarity). Note: Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome (By similarity).

TISSUE SPECIFICTY: Widespread; most abundant in skeletal muscle. Isoform 8 is detected in brain. Isoform 9 is detected in brain, placenta, lung and kidney. Isoform 11 is detected in heart and skeletal muscle.

PTM: Isoform 2 and isoform 11 are activated by phosphorylation (in vitro), but not isoform 8. Isoform 7 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59.

INVOLVEMENT IN DISEASE: Note=Genetic variations in PDE4D might be associated with susceptibility to stroke. Ref.16 states that association with stroke has to be considered with caution.

SEQUENCE SIMLARITIES: Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.

Molecular Weight

~ 59, 68, 85, 90, and 105 kDa observed. UniProtKB/Swiss-Prot Q08499 (PDE4D_HUMAN) entry describes 12 isoforms produced by alternative splicing: Isoform 1 at 91.115 kDa, Isoform 2 at 76.369 kDa, Isoform 3 at 68.607 kDa, Isoform 4 at 66.376 kDa, Isoform 5 at 57.792 kDa, Isoform 6 at 84.428 kDa, Isoform 7 at 23.839 kDa, Isoform 8 at 59.113 kDa, Isoform 9 at 77.705 kDa, Isoform 10 at 76.816 kDa, Isoform 11 at 84.662 kDa, and Isoform 12 at 24.429 kDa.

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in rat brain tissue lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected PDE4D on 10 µg of rat brain tissue lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in rat brain tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected PDE4D on 10 µg of rat brain tissue lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-PDE4D (rabbit polyclonal)

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