Anti-Myosin Heavy Chain (MyHC), clone A4.1025

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, R, M, Zebrafish, Dr, Rb	IHC, WB	M	Ascites	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, R, M, Zebrafish, Dr, Rb

IHC, WB

Ascites

Monoclonal Antibody

Description
Catalogue Number	05-716
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-Myosin Heavy Chain Antibody, clone A4.1025
Alternate Names	Myosin heavy chain 1 Myosin heavy chain 2x Myosin heavy chain IIx/d Myosin heavy chain, skeletal muscle, adult 1 myosin, heavy chain 1, skeletal muscle, adult myosin, heavy polypeptide 1, skeletal muscle, adult
Background Information	Myosin is one of the two major components of muscle tissue responsible for contraction. In muscle cells myosin is arranged as thick filamentous structures that are arranged in parallel to thin filamentous structures composed of actin. Muscle contraction is actuated by the coupling and uncoupling of thin and thick filaments through a chemical linkage mediated by ATP. The coupling and uncoupling is arranged such that the filaments slide in one direction when coupled and in reverse direction when uncoupled or released. Myosin and actin also serve to facilitate motility in non-muscle cells such as in slime molds where shorter yet similar filamentous structures work together. An increasing number of different myosins are being described. Myosin I is a low molecular weight myosin found in protozoa and does not self-assemble. Myosin II is the classic form found in muscle cells that self assemble into thick bundles. Myosin II is a multimeric protein that is composed of two heavy chains and two light chains.

Description

Catalogue Number

05-716

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-Myosin Heavy Chain Antibody, clone A4.1025

Alternate Names

Myosin heavy chain 1
Myosin heavy chain 2x
Myosin heavy chain IIx/d
Myosin heavy chain, skeletal muscle, adult 1
myosin, heavy chain 1, skeletal muscle, adult
myosin, heavy polypeptide 1, skeletal muscle, adult

Background Information

Myosin is one of the two major components of muscle tissue responsible for contraction. In muscle cells myosin is arranged as thick filamentous structures that are arranged in parallel to thin filamentous structures composed of actin. Muscle contraction is actuated by the coupling and uncoupling of thin and thick filaments through a chemical linkage mediated by ATP. The coupling and uncoupling is arranged such that the filaments slide in one direction when coupled and in reverse direction when uncoupled or released. Myosin and actin also serve to facilitate motility in non-muscle cells such as in slime molds where shorter yet similar filamentous structures work together. An increasing number of different myosins are being described. Myosin I is a low molecular weight myosin found in protozoa and does not self-assemble. Myosin II is the classic form found in muscle cells that self assemble into thick bundles. Myosin II is a multimeric protein that is composed of two heavy chains and two light chains.

Product Information
Format	Ascites
Control	RIPA lysates from C2C12 cells
Presentation	Unpurified mouse ascites monoclonal IgG in buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol. Liquid at -20°C.

Product Information

Format

Ascites

Control

RIPA lysates from C2C12 cells

Presentation

Unpurified mouse ascites monoclonal IgG in buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol. Liquid at -20°C.

Applications
Application	Anti-Myosin Heavy Chain Antibody, clone A4.1025 is an antibody against Myosin Heavy Chain for use in IH & WB.
Key Applications	Immunohistochemistry Western Blotting
Application Notes	Immunohistochemistry: This antibody has been reported by an independent laboratory to detect myosin heavy chain in zebrafish embryos and skeletal muscle sections fixed with 4% paraformaldehyde in a previous lot (Blagden, C.S., 1997; Cho, M., 1994).

Applications

Application

Anti-Myosin Heavy Chain Antibody, clone A4.1025 is an antibody against Myosin Heavy Chain for use in IH & WB.

Key Applications

Immunohistochemistry
Western Blotting

Application Notes

Immunohistochemistry:
This antibody has been reported by an independent laboratory to detect myosin heavy chain in zebrafish embryos and skeletal muscle sections fixed with 4% paraformaldehyde in a previous lot (Blagden, C.S., 1997; Cho, M., 1994).

Biological Information
Immunogen	Partially purified protein from adult human skeletal muscle myosin. Clone A4.1025.
Clone	A4.1025
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody recognizes myosin heavy chain, Mr ~220 kDa. Immunoblotting with muscle cell lysates will often show several close bands from different myosin heavy chain genes, as well as degradation products.
Isotype	IgG
Species Reactivity	Human Rat Mouse Zebrafish Drosophila Rabbit
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_005963
Entrez Gene Summary	Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development.
Gene Symbol	MYH1 MyHC-2X/D MGC133384 MYHa MyHC-IIx/d MyHC-2x Myosin-1 MYHSA1
Purification Method	Unpurified
UniProt Number	P12882
UniProt Summary	FUNCTION: SwissProt: P12882 # Muscle contraction. SIZE: 1939 amino acids; 223115 Da SUBUNIT: Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). SUBCELLULAR LOCATION: Cytoplasm, myofibril. Note=Thick filaments of the myofibrils. DOMAIN: SwissProt: P12882 The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils. SIMILARITY: Contains 1 IQ domain. & Contains 1 myosin head-like domain. MISCELLANEOUS: Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
Molecular Weight	~220 kDa

Biological Information

Immunogen

Partially purified protein from adult human skeletal muscle myosin. Clone A4.1025.

Clone

A4.1025

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

This antibody recognizes myosin heavy chain, Mr ~220 kDa. Immunoblotting with muscle cell lysates will often show several close bands from different myosin heavy chain genes, as well as degradation products.

Isotype

IgG

Species Reactivity

Human
Rat
Mouse
Zebrafish
Drosophila
Rabbit

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_005963

Entrez Gene Summary

Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development.

Gene Symbol

MYH1
MyHC-2X/D
MGC133384
MYHa
MyHC-IIx/d
MyHC-2x
Myosin-1
MYHSA1

Purification Method

Unpurified

UniProt Number

P12882

UniProt Summary

FUNCTION: SwissProt: P12882 # Muscle contraction.
SIZE: 1939 amino acids; 223115 Da
SUBUNIT: Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).
SUBCELLULAR LOCATION: Cytoplasm, myofibril. Note=Thick filaments of the myofibrils.
DOMAIN: SwissProt: P12882 The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
SIMILARITY: Contains 1 IQ domain. & Contains 1 myosin head-like domain.
MISCELLANEOUS: Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

Molecular Weight

~220 kDa

Product Usage Statements
Quality Assurance	Routinely evaluated by Western Blot on RIPA lysates from C2C12 cells. Western Blot Analysis: 1:1000-1:5000 dilution of this lot detected Myosin Heavy Chain (MyHC) in RIPA lysates from Rabbit skeletal muscle cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated by Western Blot on RIPA lysates from C2C12 cells.

Western Blot Analysis:
1:1000-1:5000 dilution of this lot detected Myosin Heavy Chain (MyHC) in RIPA lysates from Rabbit skeletal muscle cells.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20ºC from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20ºC from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

Packaging Information
Material Size	200 µL

Packaging Information

Material Size

200 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Myosin Heavy Chain (MyHC), clone A4.1025

浏览了该商品的用户最终购买

浏览了该商品的用户还浏览了

特卖

重要规格表

我要咨询