CpG Wiz® p15 Amplification Kit

Description
Catalogue Number	S7802
Brand Family	Chemicon®
Trade Name	CpG Wiz Chemicon
Description	CpG WIZ® p15 -Methylation specific PCR assay
Overview	Aberrant methylation of the p15 promoter region has been shown to cause inactivation in several gliomas and leukemias. The CpG WIZ® p15 Amplification Kit is used for determining the methylation status of the p15 promoter by methylation-specific PCR (MSP). The kit contains primers targeted to regions of the p15 promoter where the sequences are most divergent after bisulfite treatment. PCR parameters have been identified so that all primer sets in the kit amplify template under the same conditions. Control genomic DNA samples (methylated and unmethylated) for p15* are also included. Methylation-specific PCR (MSP) is a new technology for sensitive detection of abnormal gene methylation utilizing small amounts of DNA (Herman, 1996 ). This process employs an initial bisulfite reaction to modify the DNA, followed by PCR amplification with specific primers designed to distinguish methylated from unmethylated DNA. *p15 is covered under U.S. patents 5,994,095, US 6,140,473, 6,180,776, 6,210,949 and 6,218,146.

Description

Catalogue Number

S7802

Brand Family

Chemicon®

Trade Name

CpG Wiz
Chemicon

Description

CpG WIZ® p15 -Methylation specific PCR assay

Overview

Aberrant methylation of the p15 promoter region has been shown to cause inactivation in several gliomas and leukemias. The CpG WIZ® p15 Amplification Kit is used for determining the methylation status of the p15 promoter by methylation-specific PCR (MSP). The kit contains primers targeted to regions of the p15 promoter where the sequences are most divergent after bisulfite treatment. PCR parameters have been identified so that all primer sets in the kit amplify template under the same conditions. Control genomic DNA samples (methylated and unmethylated) for p15* are also included.

Methylation-specific PCR (MSP) is a new technology for sensitive detection of abnormal gene methylation utilizing small amounts of DNA (Herman, 1996 ). This process employs an initial bisulfite reaction to modify the DNA, followed by PCR amplification with specific primers designed to distinguish methylated from unmethylated DNA.

*p15 is covered under U.S. patents 5,994,095, US 6,140,473, 6,180,776, 6,210,949 and 6,218,146.

Applications
Application	The CpG WIZ p15 Amplification Kit is used for determining the methylation status of the p15 promoter by methylation-specific PCR (MSP).
Key Applications	PCR
Application Notes	Principles of the Technique Use of either the CpGenome™ DNA Modification Kit (Cat. No. S7820) or the CpGENOME™ Fast DNA Modification Kit (Cat. No. S7824) facilitates the initial bisulfite reactions, while the The CpG WIZ® p15 Amplification Kit contains the reagents required for the gene-specific PCR amplification reactions.

Applications

Application

The CpG WIZ p15 Amplification Kit is used for determining the methylation status of the p15 promoter by methylation-specific PCR (MSP).

Key Applications

Application Notes

Principles of the Technique

Use of either the CpGenome™ DNA Modification Kit (Cat. No. S7820) or the CpGENOME™ Fast DNA Modification Kit (Cat. No. S7824) facilitates the initial bisulfite reactions, while the The CpG WIZ® p15 Amplification Kit contains the reagents required for the gene-specific PCR amplification reactions.

Biological Information
Species Reactivity	Human
Entrez Gene Number	NM_004936.3 NM_078487.2
Entrez Gene Summary	This gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported.
Gene Symbol	CDKN2B TP15 INK4B p15INK4b p14-INK4b P15 MTS2 p15-INK4b MTS-2 p14_INK4B p15INK4B CDK4I p15_INK4B
UniProt Number	P42772
UniProt Summary	FUNCTION: SwissProt: P42772 # Interacts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest. SIZE: 138 amino acids; 14722 Da SUBUNIT: Heterodimer of CDKN2B with CDK4 or CDK6. DISEASE:SwissProt: P42772 # Defects in CDKN2B are involved in tumor formation. SIMILARITY: SwissProt: P42772 ## Belongs to the CDKN2 cyclin-dependent kinase inhibitor family. & Contains 4 ANK repeats.

Biological Information

Species Reactivity

Human

Entrez Gene Number

Entrez Gene Summary

This gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported.

Gene Symbol

CDKN2B
TP15
INK4B
p15INK4b
p14-INK4b
P15
MTS2
p15-INK4b
MTS-2
p14_INK4B
p15INK4B
CDK4I
p15_INK4B

UniProt Number

P42772

UniProt Summary

FUNCTION: SwissProt: P42772 # Interacts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest.
SIZE: 138 amino acids; 14722 Da
SUBUNIT: Heterodimer of CDKN2B with CDK4 or CDK6.
DISEASE:SwissProt: P42772 # Defects in CDKN2B are involved in tumor formation.
SIMILARITY: SwissProt: P42772 ## Belongs to the CDKN2 cyclin-dependent kinase inhibitor family. & Contains 4 ANK repeats.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Packaging Information
Material Size	25 assays

Packaging Information

Material Size

25 assays

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

CpG Wiz® p15 Amplification Kit

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