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Ms X Hu Vimentin

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货号：MAB1687

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, Mk ICC, IH(P) M Ascites Monoclonal Antibody
Description
Catalogue Number MAB1687
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Vimentin Antibody, clone 43BE8
References
Product Information
Format Ascites
Presentation Liquid
Applications
Application This Anti-Vimentin Antibody, clone 43BE8 is validated for use in IC, IH(P) for the detection of Vimentin.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
Application Notes Immunohistochemistry: 1:500-1:2,000 on paraffin embedded sections (ABC peroxidase) fixed with alcohol (e.g. Carnoy) with a 2" pronase digestion. Aldehyde fixation is not recommended.

Immunocytochemistry: 1:100-1:200 cells in vitro (IFA) fixed in ice cold absolute methanol or acetone.

Optimal working dilutions must be determined by the end user.
Biological Information
Clone 43BE8
Host Mouse
Specificity Vimentin (58kD). Reacts in immunocytochemical assays with all mesenchymal cells (fibroblasts, Scwann cells, endothelial cells, smooth muscle cells, etc.).
Isotype IgM
Species Reactivity
  • Human
  • Monkey
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]
Gene Symbol
  • VIM
  • FLJ36605
  • Vimentin.
  • vimentin
UniProt Number
UniProt Summary FUNCTION: SwissProt: P08670 # Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.
SIZE: 466 amino acids; 53652 Da
SUBUNIT: Homopolymer. Component of a complex composed at least of ACTB, AP2M1, AP2A1, AP2A2, MEGF10 and VIM. Interacts with HCV core protein.
TISSUE SPECIFICITY: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines.
PTM: One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized.
SIMILARITY: SwissProt: P08670 ## Belongs to the intermediate filament family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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