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Anti-JARID1D (rabbit polyclonal)

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货号：ABE203

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M WB, ICC, IP Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number ABE203
Description Anti-JARID1D Antibody
Alternate Names
  • Lysine-specific demethylase 5D
  • Histocompatibility Y antigen
  • H-Y
  • Histone demethylase JARID1D
  • Jumonji/ARID domain-containing protein 1D
  • Protein SmcY
Background Information Also known as Lysine-specific demethylase 5D, this histone demethylase is specific for 'Lys-4' of histone H3 and is known to play a central role in histone function and spermatogenesis. It demethylates trimethylated and dimethylated, but not monomethylated H3 'Lys-4'. Uniprot.org states two different isoforms of this enzyme.
References
Product Information
Format Affinity Purified
Control
  • Human testis tissue lysate.
Presentation Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application Use Anti-JARID1D Antibody (rabbit polyclonal antibody) validated in WB, ICC, IP to detect JARID1D also known as Lysine-specific demethylase 5D, Histocompatibility Y antigen, H-Y, Histone demethylase JARID1D.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunoprecipitation
Application Notes Immunocytochemistry Analysis: 2 µg/mL from a representative lot detected JARID1D in mESCs.

Immunoprecipitation Analysis: A representative lot immunoprecipitated JARID1D in human testis tissue lysate.
Biological Information
Immunogen KLH-conjugated linear peptide corresponding to human JARID1D.
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Species Reactivity
  • Human
  • Mouse
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene encodes a protein containing zinc finger domains. A short peptide derived from this protein is a minor histocompatibility antigen which can lead to graft rejection of male donor cells in a female recipient. Alternative splicing results in multiple transcript variants.
Gene Symbol
  • KDM5D
  • HY
  • HYA
  • JARID1D
  • KIAA0234
  • SMCY
Purification Method Affinity Purfied
UniProt Number
UniProt Summary FUNCTION: Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. May play a role in spermatogenesis.

COFACTOR: Ascorbate. Binds 1 Fe2+ ion per subunit.

SUBUNIT STRUCTURE: Interacts with PCGF6 and MSH5.

SUBCELLULAR LOCATION: Nucleus.
Molecular Weight ~174 kDa observed. Two isoforms may be observed due to alternative splicing at a molecular weight of ~167 kDa and ~174 kDa in some cell lysates. An uncharacterized band at a molecular weight of ~255 kDa may be observed in some cell lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in human testis tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected JARID1D in 10 µg of human testis tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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