Rat Anti-Mouse PECAM-1 [CD31] Monoclonal Antibody - 炼石商城

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Rat Anti-Mouse PECAM-1 [CD31] Monoclonal Antibody

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货号：CBL1337

品牌：Millipore 密理博

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
M	FC, IHC, IP, FUNC	R	Purified	Monoclonal Antibody

Description
Catalogue Number	CBL1337
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-PECAM-1 Antibody, clone 390
Alternate Names	CD31
Background Information	CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1) is a type I integral membrane glycoprotein, and a member of the immunoglobulin superfamily of cell surface receptors. PECAM-1 is constitutively expressed on the surface of endothelial cells where it is concentrated at cell junctions. PECAM-1 is also expressed at low levels on all leukocytes and platelets. Homotypic interaction of CD31 is important in cell-cell interaction, cell-matrix interaction and signal transduction.

References

Product Information
Format	Purified
Control	Jurkat and Raji cells, tonsil tissue
Presentation	Protein A Purified mouse immunoglobulin in 100mM borate buffered saline, pH 8.0, containing no preservative.

Applications
Application	Anti-PECAM-1 Antibody, clone 390 is an antibody against PECAM-1 for use in FC, IH & IP.
Key Applications	Flow Cytometry Immunohistochemistry Immunoprecipitation Affects Function
Application Notes	Flow Cytometry: 1 μg/10e6 cells. Immunohistochemistry: acteone fixed frozen tissue sections Immunoprecipitation Blocking of cell adhesion: CBL1337 inhibits aggregation of L cells transfected with a variant form of CD31 (DeLisser, 1993) Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	Mouse leukocyte cell line 32D
Clone	390
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rat
Specificity	CBL1337 recognizes mouse PECAM-1, a 130-140 kDa type 1 integral membrane glycoprotein. This antibody has been shown to inhibit the aggregation of L cells transfected with a variant form of PECAM-1 (DeLisser, 1993).
Isotype	IgG2aκ
Species Reactivity	Mouse
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_000442.3
Gene Symbol	PECAM1 EndoCAM GPIIA PECAM-1 CD31 CD31/EndoCAM
Purification Method	Protein A Purfied
UniProt Number	P16284
UniProt Summary	FUNCTION: SwissProt: P16284 # This protein is a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. SIZE: 738 amino acids; 82536 Da SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Long isoform predominates all tissues examined, isoform Delta12 was detected only in trachea and isoform Delta14-15 only in lung, isoform Delta14 was detected in all tissues examined with the strongest expression in heart. PTM: Phosphorylated on Ser and Tyr residues after cellular activation. SIMILARITY: SwissProt: P16284 ## Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

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Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Packaging Information
Material Size	500 µg

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