Anti-Reelin, clone G10, Alexa Fluor® 488 Conjugate

Species Reactivity	Key Applications	Host	Format	Antibody Type
M, R	ICC, IHC	M	AlexaFluor®488	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

M, R

ICC, IHC

AlexaFluor®488

Monoclonal Antibody

Description
Catalogue Number	MAB5364A4
Description	Anti-Reelin Antibody, clone G10, Alexa Fluor® 488 Conjugate
Alternate Names	Reelin
Background Information	Reelin, a glycoprotein, plays a crucial role during embryonic neuronal migration, primarily as a regulator of corticogenesis and neuronal cell positioning in the prenatal period. In adults, reelin may act as a modulator of synaptic plasticity. Reelin has been implicated to play a role in several brain diseases including schizphrenia, Alzheimers's disease, temporal lobe epilespy, and autism. Reelin is found in the brain, spinal cord, blood, and other body organs and tissues.

Description

Catalogue Number

MAB5364A4

Description

Anti-Reelin Antibody, clone G10, Alexa Fluor® 488 Conjugate

Alternate Names

Reelin

Background Information

Reelin, a glycoprotein, plays a crucial role during embryonic neuronal migration, primarily as a regulator of corticogenesis and neuronal cell positioning in the prenatal period. In adults, reelin may act as a modulator of synaptic plasticity. Reelin has been implicated to play a role in several brain diseases including schizphrenia, Alzheimers's disease, temporal lobe epilespy, and autism. Reelin is found in the brain, spinal cord, blood, and other body organs and tissues.

Product Information
Format	AlexaFluor®488
Control	Adult mouse brain tissue
Presentation	Purified mouse monoclonal IgG1 conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Product Information

Format

AlexaFluor®488

Control

Adult mouse brain tissue

Presentation

Purified mouse monoclonal IgG1 conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Applications
Application	Anti-Reelin Antibody, clone G10, Alexa Fluor 488 Conjugate detects level of Reelin & has been published & validated for use in IC, IH.
Key Applications	Immunocytochemistry Immunohistochemistry
Application Notes	Immunocytochemistry Analysis: A 1:400 dilution from a representative lot detected Reelin in rat E18 primary cortex cells.

Applications

Application

Anti-Reelin Antibody, clone G10, Alexa Fluor 488 Conjugate detects level of Reelin & has been published & validated for use in IC, IH.

Key Applications

Immunocytochemistry
Immunohistochemistry

Application Notes

Immunocytochemistry Analysis: A 1:400 dilution from a representative lot detected Reelin in rat E18 primary cortex cells.

Biological Information
Immunogen	Recombinant protein corresponding to rat Reelin.
Clone	G10
Host	Mouse
Isotype	IgG
Species Reactivity	Mouse Rat
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_536319
Entrez Gene Summary	This gene encodes a large secreted extracellular matrix protein thought to control cell-cell interactions critical for cell positioning and neuronal migration during brain development. This protein may be involved in schizophrenia, autism, bipolar disorder, major depression and in migration defects associated with temporal lobe epilepsy. Mutations of this gene are associated with autosomal recessive lissencephaly with cerebellar hypoplasia. Two transcript variants encoding distinct isoforms have been identified for this gene. Other transcript variants have been described but their full length nature has not been determined.
Gene Symbol	Reln
Purification Method	Protein A Purfied
UniProt Number	P58751
UniProt Summary	FUNCTION: Extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. Regulates microtubule function in neurons and neuronal migration. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and ApoER2 induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation (By similarity). SIZE: 3460 amino acids; 388402 Da SUBUNIT: Binds to the ectodomains of VLDLR and ApoER2 (By similarity). SUBCELLULAR LOCATION: Secreted (By similarity). TISSUE SPECIFICITY: Abundantly produced during brain ontogenesis by the Cajal-Retzius cells and other pioneer neurons located in the telencephalic marginal zone and by granule cells of the external granular layer of the cerebellum. In adult brain, preferentially expressed in GABAergic interneurons of prefrontal cortices, temporal cortex, hippocampus and glutamatergic granule cells of cerebellum. Also expressed in fetal and adult liver.DEVELOPMENTAL STAGE: Expressed in fetal and postnatal brain and liver. Expression in postnatal human brain is high in the cerebellum. DOMAIN: The basic C-terminal region is essential for secretion (By similarity). DISEASE: Defects in RELN are the cause of autosomal recessive lissencephaly with cerebellar hypoplasia [MIM:257320]; also known as Norman-Roberts syndrome. Some patients also displayed persistent lymphedema neonatally, and one showed accumulation of chlyous or fatty, ascites fluid. & Defects in RELN may contribute to susceptibility to schizophrenia. Expression of the protein is reduced to about 50% in patients with schizophrenia. & Defects in RELN may predispose to autistic disorder. A polymorphic GGC triplet repeat located in the 5'-UTR region of RELN gene, which harbors in the normal population 8 to 10 repeats, is significantly increased in autistic patients to carry 4 to 23 additional repeats. SIMILARITY: Belongs to the reelin family. & Contains 15 BNR repeats. & Contains 8 EGF-like domains. & Contains 1 reelin domain.
Molecular Weight	388 kDa calculated

Biological Information

Immunogen

Recombinant protein corresponding to rat Reelin.

Clone

G10

Host

Mouse

Isotype

IgG

Species Reactivity

Mouse
Rat

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_536319

Entrez Gene Summary

This gene encodes a large secreted extracellular matrix protein thought to control cell-cell interactions critical for cell positioning and neuronal migration during brain development. This protein may be involved in schizophrenia, autism, bipolar disorder, major depression and in migration defects associated with temporal lobe epilepsy. Mutations of this gene are associated with autosomal recessive lissencephaly with cerebellar hypoplasia. Two transcript variants encoding distinct isoforms have been identified for this gene. Other transcript variants have been described but their full length nature has not been determined.

Gene Symbol

Reln

Purification Method

Protein A Purfied

UniProt Number

P58751

UniProt Summary

FUNCTION: Extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. Regulates microtubule function in neurons and neuronal migration. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and ApoER2 induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation (By similarity).

SIZE: 3460 amino acids; 388402 Da

SUBUNIT: Binds to the ectodomains of VLDLR and ApoER2 (By similarity).

SUBCELLULAR LOCATION: Secreted (By similarity).

TISSUE SPECIFICITY: Abundantly produced during brain ontogenesis by the Cajal-Retzius cells and other pioneer neurons located in the telencephalic marginal zone and by granule cells of the external granular layer of the cerebellum. In adult brain, preferentially expressed in GABAergic interneurons of prefrontal cortices, temporal cortex, hippocampus and glutamatergic granule cells of cerebellum. Also expressed in fetal and adult liver.DEVELOPMENTAL STAGE: Expressed in fetal and postnatal brain and liver. Expression in postnatal human brain is high in the cerebellum.

DOMAIN: The basic C-terminal region is essential for secretion (By similarity).

DISEASE: Defects in RELN are the cause of autosomal recessive lissencephaly with cerebellar hypoplasia [MIM:257320]; also known as Norman-Roberts syndrome. Some patients also displayed persistent lymphedema neonatally, and one showed accumulation of chlyous or fatty, ascites fluid. & Defects in RELN may contribute to susceptibility to schizophrenia. Expression of the protein is reduced to about 50% in patients with schizophrenia. & Defects in RELN may predispose to autistic disorder. A polymorphic GGC triplet repeat located in the 5'-UTR region of RELN gene, which harbors in the normal population 8 to 10 repeats, is significantly increased in autistic patients to carry 4 to 23 additional repeats.

SIMILARITY: Belongs to the reelin family. & Contains 15 BNR repeats. & Contains 8 EGF-like domains. & Contains 1 reelin domain.

Molecular Weight

388 kDa calculated

Product Usage Statements
Quality Assurance	Evaluated by Immunohistochemistry in adult mouse brain tissue. Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected Reelin in adult mouse brain tissue.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Immunohistochemistry in adult mouse brain tissue.

Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected Reelin in adult mouse brain tissue.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Storage and Shipping Information

Storage Conditions

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Reelin, clone G10, Alexa Fluor® 488 Conjugate

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