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Anti-HOXD4, clone 8E11.1 (mouse monoclonal)

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号:MABE157

牌:Millipore 密理博

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Replacement Information

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
R, M WB M Purified Monoclonal Antibody
Description
Catalogue Number MABE157
Description Anti-HOXD4 Antibody, clone 8E11.1
Alternate Names
  • Homeobox protein Hox-D4
  • Homeobox protein HHO.C13
  • Homeobox protein Hox-4B
  • Homeobox protein Hox-5.1
Background Information This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, located on different chromosomes, consisting of 9 to 11 genes arranged in tandem. It is reported that all Hox paralog group 4 members, A4, B4, C4, and D4, have similar effects on hematopoietic stem and progenitor self-renewal in vitro. It is shown that each paralog group 4 member both promotes proliferation and inhibits differentiation, thereby enabling the exponential expansion of hematopoietic progenitors from c-kit(+)/CD41(+) cell fractions of day 6 murine embryoid bodies.
References
Product Information
Format Purified
Control
  • Rat kidney tissue lysate
Presentation Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
Applications
Application Anti-HOXD4 Antibody, clone 8E11.1 is a Mouse Monoclonal Antibody for detection of HOXD4 also known as Homeobox protein Hox-D4 & has been validated in WB.
Key Applications
  • Western Blotting
Biological Information
Immunogen GST-tagged recombinant protein corresponding to human HOXD4.
Clone 8E11.1
Host Mouse
Isotype IgMκ
Species Reactivity
  • Rat
  • Mouse
Species Reactivity Note Demonstrated to react with Rat and Mouse. Predicted to react with Human based on immunogen design.
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, located on different chromosomes, consisting of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXD genes located at 2q31-2q37 chromosome regions. Deletions that removed the entire HOXD gene cluster or 5' end of this cluster have been associated with severe limb and genital abnormalities. The protein encoded by this gene may play a role in determining positional values in developing limb buds. Alternatively spliced variants have been described but their full length nature has not been determined.
Gene Symbol
  • HOXD4
  • HOX4B
Purification Method Purified by Ion-Exchange Chromatography
UniProt Number
UniProt Summary FUNCTION: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.

SUBCELLULAR LOCATION: Nucleus.
Molecular Weight ~38 kDa observed. 28 kDa calculated. Observed molecular weight is ~38 kDa due to the proline-rich N-terminus of HOXD-4. (Phelan, M.L., Sadoul, R., Featherstone, M.S. (1994). Mol Cell Biol. 14(8):5066-5075.)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in rat kidney tissue lysate.

Western Blot Analysis: A 1:1,2000 dilution of this antibody detected HOXD4 in 10 µg of rat kidney tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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