Rb Anti-ASIC4

Species Reactivity	Key Applications	Host	Format	Antibody Type
R	WB	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	AB9596-200UL
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-ASIC4 Antibody

Description

Catalogue Number

AB9596-200UL

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-ASIC4 Antibody

Product Information
Format	Affinity Purified
Control	Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Presentation	Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Product Information

Format

Affinity Purified

Control

Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Presentation

Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Applications
Application	Anti-ASIC4 Antibody detects level of ASIC4 & has been published & validated for use in WB.
Key Applications	Western Blotting
Application Notes	Western blot: 1:200-1:500 using ECL on rat DRG lysate, rat brain and the ND7/23 cell line. Dilutions should be made using a carrier protein such as BSA (1-3%). Optimal working dilutions must be determined by the end user.SUGGESTED WESTERN BLOT PROTOCOL 1. Mix the samples (organ membranes: 50 μg/lane; transfected cells: 500,000 cells/lane) with sample-buffer X 2, and heat 10 min at 70°C. 2. 5-50 μL applied to Minigel lane (0.75-1.5 mm width) and run at standard conditions. (60 mA for 2 1.5 mm Minigel gels, 1.4 h). It is suggested that you run 5-15% acrylamide (37.5:1 acrylamide:bisacrysmide) minigel (1.5 mm width) at 30 mA/gel ~1-1.5 hours. 3. Transfer in semi-dry system under standard conditions (3 h 100 mA for two minigel gels) 4. Stain the transferred bands with Chemicon BLOT-FastStain (Catalog Number 2076). 5. Destain with deionized water. 6. Block with 5% non-fat milk (Marvel or Carnation) in PBS, and 0.025 % sodium azide, overnight at 2-8°C. The non-fat milk should be dissolved freshly, centrifuged 10,000 rpm for 10 min, and filtered through glass filter (Gelman Acrodisc). 7. Incubation with first antibody 2 h at room temperature or overnight at 4°C in blocking solution. The antibody preparation should be centrifuged before use (10,000 g 5 min.). Optimal working dilutions and incubation time will need to be determined by the end user. 8. Wash 4 x 10 min. with PBS-0.1% tween 20. From this stage, azide should be omitted. 9. Incubation with the secondary antibody (HRP-conjugated goat anti-rabbit antibody, for example Chemicon Catalog Number AP132P, diluted appropriately) 1 h at room temperature. 10. Wash 4 x 10 min. with PBS-0.1% tween 20. 11. Perform ECL with commercial kits (Chemilucent, Chemicon Catalog Number 2600).

Applications

Application

Anti-ASIC4 Antibody detects level of ASIC4 & has been published & validated for use in WB.

Key Applications

Western Blotting

Application Notes

Western blot: 1:200-1:500 using ECL on rat DRG lysate, rat brain and the ND7/23 cell line.

Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions must be determined by the end user.SUGGESTED WESTERN BLOT PROTOCOL

1. Mix the samples (organ membranes: 50 μg/lane; transfected cells: 500,000 cells/lane) with sample-buffer X 2, and heat 10 min at 70°C.

2. 5-50 μL applied to Minigel lane (0.75-1.5 mm width) and run at standard conditions. (60 mA for 2 1.5 mm Minigel gels, 1.4 h). It is suggested that you run 5-15% acrylamide (37.5:1 acrylamide:bisacrysmide) minigel (1.5 mm width) at 30 mA/gel ~1-1.5 hours.

3. Transfer in semi-dry system under standard conditions (3 h 100 mA for two minigel gels)

4. Stain the transferred bands with Chemicon BLOT-FastStain (Catalog Number 2076).

5. Destain with deionized water.

6. Block with 5% non-fat milk (Marvel or Carnation) in PBS, and 0.025 % sodium azide, overnight at 2-8°C. The non-fat milk should be dissolved freshly, centrifuged 10,000 rpm for

10 min, and filtered through glass filter (Gelman Acrodisc).

7. Incubation with first antibody 2 h at room temperature or overnight at 4°C in blocking solution. The antibody preparation should be centrifuged before use (10,000 g 5 min.). Optimal working dilutions and incubation time will need to be determined by the end user.

8. Wash 4 x 10 min. with PBS-0.1% tween 20. From this stage, azide should be omitted.

9. Incubation with the secondary antibody (HRP-conjugated goat anti-rabbit antibody, for example Chemicon Catalog Number AP132P, diluted appropriately) 1 h at room temperature.

10. Wash 4 x 10 min. with PBS-0.1% tween 20.

11. Perform ECL with commercial kits (Chemilucent, Chemicon Catalog Number 2600).

Biological Information
Immunogen	Purified peptide from amino acids 7-16 of rat ASIC4 (Accession number Q9QYV9).
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Recognizes ASIC4 (H+ gated cation channel, SPASIC, ACCN4).
Species Reactivity	Rat
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_018674.3 NM_182847.1
Entrez Gene Summary	This gene belongs to the superfamily of acid-sensing ion channels, which are proton-gated, amiloride-sensitive sodium channels. These channels have been implicated in synaptic transmission, pain perception as well as mechanoperception. This gene is predominantly expressed in the pituitary gland, and was considered a candidate for paroxysmal dystonic choreoathetosis (PDC), a movement disorder, however, no correlation was found between mutations in this gene and PDC. Alternative splicing at this locus results in two transcript variants encoding different isoforms.
Gene Symbol	ACCN4 MGC24860 ASIC4 BNAC4 MGC17248
UniProt Number	Q96FT7
UniProt Summary	FUNCTION: SwissProt: Q96FT7 # Probable cation channel with high affinity for sodium. SIZE: 647 amino acids; 70033 Da SUBUNIT: Homotetramer or heterotetramer with other ASIC proteins (Probable). SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (By similarity). TISSUE SPECIFICITY: Expressed in pituitary gland. Weakly expressed in brain, vestibular system and organ of Corti. SIMILARITY: SwissProt: Q96FT7 ## Belongs to the amiloride-sensitive sodium channel family. MISCELLANEOUS: In vitro, has no proton-gated channel activity.

Biological Information

Immunogen

Purified peptide from amino acids 7-16 of rat ASIC4 (Accession number Q9QYV9).

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

Recognizes ASIC4 (H+ gated cation channel, SPASIC, ACCN4).

Species Reactivity

Antibody Type

Polyclonal Antibody

Entrez Gene Number

Entrez Gene Summary

This gene belongs to the superfamily of acid-sensing ion channels, which are proton-gated, amiloride-sensitive sodium channels. These channels have been implicated in synaptic transmission, pain perception as well as mechanoperception. This gene is predominantly expressed in the pituitary gland, and was considered a candidate for paroxysmal dystonic choreoathetosis (PDC), a movement disorder, however, no correlation was found between mutations in this gene and PDC. Alternative splicing at this locus results in two transcript variants encoding different isoforms.

Gene Symbol

ACCN4
MGC24860
ASIC4
BNAC4
MGC17248

UniProt Number

Q96FT7

UniProt Summary

FUNCTION: SwissProt: Q96FT7 # Probable cation channel with high affinity for sodium.
SIZE: 647 amino acids; 70033 Da
SUBUNIT: Homotetramer or heterotetramer with other ASIC proteins (Probable).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (By similarity).
TISSUE SPECIFICITY: Expressed in pituitary gland. Weakly expressed in brain, vestibular system and organ of Corti.
SIMILARITY: SwissProt: Q96FT7 ## Belongs to the amiloride-sensitive sodium channel family.
MISCELLANEOUS: In vitro, has no proton-gated channel activity.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Storage and Shipping Information

Storage Conditions

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Packaging Information
Material Size	200 µL

Packaging Information

Material Size

200 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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