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Anti- OREXIN-2 RECEPTOR (rabbit polyclonal)

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货号：AB3094

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M, R ELISA, IHC, WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB3094
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Orexin-2 Receptor Antibody
Alternate Names
  • OX2R
  • Hypocretin Receptor 2
References
Product Information
Format Affinity Purified
Presentation Affinity Purified 1mg/mL solution in PBS, pH 7.4 and 0.1% BSA
Applications
Application Detect Orexin-2 Receptor using this Anti-Orexin-2 Receptor Antibody validated for use in ELISA, IH & WB.
Key Applications
  • ELISA
  • Immunohistochemistry
  • Western Blotting
Application Notes Western blot: 1-10 μg/mL using Chemiluminescence technique

ELISA: 1:10,000 - 100,000 against the control peptide (Catalog Number AG794).

Optimal working dilutions must be determined by end user.
Biological Information
Immunogen A 19 aa peptide near the C-terminus (cytoplasmic domain) of rat OX2R. The immunogen peptide (Catalog Number AG794) is available to perform preabsorbtion controls.
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Rat/Human Orexin-2. Several peptides associated with feeding behavior have been reported recently. Orexins (Orexin-A and Orexin-B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus (Sakurai et al. 1998). Orexin-A and Orexin-B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage. Prepro-orexin is an 130 aa peptide with a putative 33 aa secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis (Sakurai et al. 1998). These neuropeptides bind and activate two closely related Orexin receptors--G protein coupled receptors (GPCRs) OX1R and OX2R.
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary HCRTR2 is a G-protein coupled receptor expressed exclusively in the brain. It has 64% identity with HCRTR1. HCRTR2 binds both orexin A and orexin B neuropeptides. HCRTR2 is involved in the central feedback mechanism that regulates feeding behaviour.
Gene Symbol
  • HCRTR2
  • OTTHUMP00000039969
  • Ox2r
  • OX2R
UniProt Number
UniProt Summary FUNCTION: SwissProt: O43614 # Nonselective, high-affinity receptor for both orexin-A and orexin-B neuropeptides.
SIZE: 444 amino acids; 50680 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: O43614 ## Belongs to the G-protein coupled receptor 1 family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain frozen at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 50 µg
Transport Information
Supplemental Information
Specifications

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