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Milli-Mark™ Anti-Phosphotyrosine-PE recombinant clone 4G10®

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货号：FCMAB323PE

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
Vrt FC M Phycoerythrin Monoclonal Antibody
Description
Catalogue Number FCMAB323PE
Trade Name
  • Milli-Mark
Description Milli-Mark™ Anti-Phosphotyrosine-PE Antibody, recombinant clone 4G10®
Background Information Some of the tyrosine residues can be tagged with a phosphate group by protein kinases and in its phosphorylated state, it is referred to as phosphotyrosine. Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity.
The advent of anti-phosphotyrosine antibodies is one of the significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosine phosphorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyne phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
References
Product Information
Format Phycoerythrin
Control
  • Treated versus untreated A431 cells
Presentation Purified mouse monoclonal IgG2bk conjugated to PE in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Applications
Application Milli-Mark Anti-Phosphotyrosine-PE Antibody, recombinant clone 4G10 is an antibody against Phosphotyrosine clone 4G10 for use in FC.
Key Applications
  • Flow Cytometry
Biological Information
Immunogen Phosphotyramine-KLH
Clone 4G10
Host Mouse
Specificity Antibody recognizes tyrosine-phosphorylated proteins from all species
Isotype IgG2bκ
Species Reactivity
  • Vertebrates
Species Reactivity Note Tested and passed on Human. Expected to detect tyrosyne phosphorylated proteins in all species.
Antibody Type Monoclonal Antibody
Purification Method Protein G Purified
Molecular Weight Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by flow cytometry using EGF treated versus untreated A431 cells
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain refrigerated at 2-8°C protected from light for up to 6 months from date of receipt.
Packaging Information
Material Size 100 tests
Transport Information
Supplemental Information
Specifications

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