Anti-PARP1 (cleaved form) (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, ICC	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

WB, ICC

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	ABC26
Replaces	04-576
Description	Anti-PARP1 (cleaved form) Antibody
Alternate Names	Poly [ADP-ribose] polymerase 1 PARP-1 NAD(+) ADP-ribosyltransferase 1 ADPRT 1 Poly[ADP-ribose] synthase 1 ARTD1
Background Information	Poly [ADP-ribose] polymerase 1 (UniProt: P09874; also known as EC: 2.4.2.30, PARP-1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1) is encoded by the PARP1 (also known as ADPRT, PPOL) gene (Gene ID: 142) in human. PARP1 is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair. It is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Its DNA-binding region is localized to amino acids 2-372. PARP1 contains two zinc-finger regions (aa 9-93 and 113-203). PARP is cleaved between amino acids Asp214 and Gly215 to yield two fragments: a 29 kDa (N-terminal) and a 85 kDa (C-terminal DNA-binding domain). The carboxyl-terminally located 54-kDa domain of PARP represents the NAD1-binding domain with the characteristic “PARP signature,” which includes a highly conserved sequence comprising the catalytically crucial amino acid residue Glu-988. Its catalytic activity is stimulated by noncovalent contact of the DNA-binding domain with DNA strand breaks and results in the post-translational modification of various acceptor proteins. In between the DNA binding domain and the NAD1-binding domain, it also contains a 22-kDa automodification domain. (Ref.: Alvarez-Gonzalez, R., et al. (1999). J. Biol. Chem. 274(45); 32122–32126).

Description

Catalogue Number

ABC26

Replaces

04-576

Description

Anti-PARP1 (cleaved form) Antibody

Alternate Names

Poly [ADP-ribose] polymerase 1
PARP-1
NAD(+) ADP-ribosyltransferase 1
ADPRT 1
Poly[ADP-ribose] synthase 1
ARTD1

Background Information

Poly [ADP-ribose] polymerase 1 (UniProt: P09874; also known as EC: 2.4.2.30, PARP-1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1) is encoded by the PARP1 (also known as ADPRT, PPOL) gene (Gene ID: 142) in human. PARP1 is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair. It is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Its DNA-binding region is localized to amino acids 2-372. PARP1 contains two zinc-finger regions (aa 9-93 and 113-203). PARP is cleaved between amino acids Asp214 and Gly215 to yield two fragments: a 29 kDa (N-terminal) and a 85 kDa (C-terminal DNA-binding domain). The carboxyl-terminally located 54-kDa domain of PARP represents the NAD1-binding domain with the characteristic “PARP signature,” which includes a highly conserved sequence comprising the catalytically crucial amino acid residue Glu-988. Its catalytic activity is stimulated by noncovalent contact of the DNA-binding domain with DNA strand breaks and results in the post-translational modification of various acceptor proteins. In between the DNA binding domain and the NAD1-binding domain, it also contains a 22-kDa automodification domain. (Ref.: Alvarez-Gonzalez, R., et al. (1999). J. Biol. Chem. 274(45); 32122–32126).

Product Information
Format	Affinity Purified
Control	Jurkat cell lysate untreated and Camptothecin-treated
Presentation	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Affinity Purified

Control

Jurkat cell lysate untreated and Camptothecin-treated

Presentation

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	This Anti-PARP1 (cleaved form) Antibody is validated for use in WB, IC for the detection of PARP1 (cleaved form).
Key Applications	Western Blotting Immunocytochemistry
Application Notes	Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected cleaved PARP1 in staurosporine-untreated and staurosporine-treated HeLa cells.

Applications

Application

This Anti-PARP1 (cleaved form) Antibody is validated for use in WB, IC for the detection of PARP1 (cleaved form).

Key Applications

Western Blotting
Immunocytochemistry

Application Notes

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected cleaved PARP1 in staurosporine-untreated and staurosporine-treated HeLa cells.

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to the DNA binding domain of human PARP1.
Epitope	DNA binding domain
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	This antibody recognizes cleaved PARP1 at the DNA binding domain.
Species Reactivity	Human
Species Reactivity Note	Demonstrated to react with Human. Other homologies: Rat and Mouse (90% sequence homology).
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_001609
Entrez Gene Summary	This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq].
Gene Symbol	PARP1 ADPRT PPOL
Purification Method	Affinity Purfied
UniProt Number	P09874
UniProt Summary	FUNCTION: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. CATALYTIC ACTIVITY: NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor. Ref.27 SUBUNIT STRUCTURE: Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and ZNF423 (By similarity). Interacts (when poly-ADP-ribosylated) with CHD1L. Interacts with the DNA polymerase alpha catalytic subunit POLA1; this interaction functions as part of the control of replication fork progression. Interacts with RNF4. SUBCELLULAR LOCATION: Nucleus. PTM: Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR. Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites. S-nitrosylated, leading to inhibit transcription regulation activity (By similarity). MISCELLANEOUS: The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. SEQUENCE SIMILARITIES: Contains 1 BRCT domain. Contains 1 PARP alpha-helical domain. Contains 1 PARP catalytic domain. Contains 2 PARP-type zinc fingers.
Molecular Weight	~89 kDa observed

Biological Information

Immunogen

KLH-conjugated linear peptide corresponding to the DNA binding domain of human PARP1.

Epitope

DNA binding domain

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

This antibody recognizes cleaved PARP1 at the DNA binding domain.

Species Reactivity

Human

Species Reactivity Note

Demonstrated to react with Human.
Other homologies: Rat and Mouse (90% sequence homology).

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_001609

Entrez Gene Summary

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq].

Gene Symbol

PARP1
ADPRT
PPOL

Purification Method

Affinity Purfied

UniProt Number

P09874

UniProt Summary

FUNCTION: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.

CATALYTIC ACTIVITY: NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor. Ref.27

SUBUNIT STRUCTURE: Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and ZNF423 (By similarity). Interacts (when poly-ADP-ribosylated) with CHD1L. Interacts with the DNA polymerase alpha catalytic subunit POLA1; this interaction functions as part of the control of replication fork progression. Interacts with RNF4.

SUBCELLULAR LOCATION: Nucleus.

PTM: Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.

Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.

S-nitrosylated, leading to inhibit transcription regulation activity (By similarity).

MISCELLANEOUS: The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units.

SEQUENCE SIMILARITIES: Contains 1 BRCT domain.

Contains 1 PARP alpha-helical domain.

Contains 1 PARP catalytic domain.

Contains 2 PARP-type zinc fingers.

Molecular Weight

~89 kDa observed

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in Jurkat cell lysate, untreated and Camptothecin-treated. Western Blot Analysis: A 1:50,000 dilution of this antibody detected cleaved PARP1 in 10 µg of Jurkat cell lysate, untreated and Camptothecin-treated.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in Jurkat cell lysate, untreated and Camptothecin-treated.

Western Blot Analysis: A 1:50,000 dilution of this antibody detected cleaved PARP1 in 10 µg of Jurkat cell lysate, untreated and Camptothecin-treated.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-PARP1 (cleaved form) (rabbit polyclonal)

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