Anti-SHPS-1, clone P84 (rat monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
M	WB, ICC, IHC, FUNC	R	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

WB, ICC, IHC, FUNC

Purified

Monoclonal Antibody

Description
Catalogue Number	MABS164
Description	Anti-SHPS-1 Antibody, clone P84
Alternate Names	Tyrosine-protein phosphatase non-receptor type substrate 1 SHP substrate 1 Brain Ig-like molecule with tyrosine-based activation motifs Bit CD172 antigen-like family member A Inhibitory receptor SHPS-1 MyD-1 antigen Signal-regulatory protein alpha-1 Sirp-alpha-1 mSIRP-alpha1 p84 CD172a
Background Information	SHPS-1 (also known as P84, BIT, and SIRP) is a neuronal membrane glycoprotein that promotes the attachment and neurite outgrowth of cultured murine cerebellar cells. Because of its heterophilic adhesive properties and its localization at synaptogenesis sites, SHPS-1 is suggested play a role in the regulation of synapse formation or maintenance. As a member of a phosphatase binding protein family, SHPS-1 shares identical homology with murine SHPS-1 cDNA. It is involved in receptor tyrosine kinase signaling that is found at synapses in the mammalian central nervous system and in non-neural tissues. It is reported that integrin-associated protein (IAP/CD47) is a predominant binding partner for P84. Upon phosphorylation of its cytoplasmic region, SHPS-1 binds to the protein-tyrosine phosphatase SHPS-2. Formation of the SHPS-1-SHP-2 complex is also implicated in the regulation of cellular migration. SHPS-1 and its ligand CD47 constitute an intercellular recognition system that contributes to the inhibition of cell migration via cell-cell contact. The ectodomain of SHPS-1 is shown to be excised from cells in a reaction that is likely mediated by a metalloproteinase. It is demonstrated that forced expression of a SHPS-1 mutant resistant to ectodomain shedding impaires cell migration, cell spreading, actin cytoskeleton reorganization, and paxillin- and FAK- tyrosine phosphorylation.

Description

Catalogue Number

MABS164

Description

Anti-SHPS-1 Antibody, clone P84

Alternate Names

Tyrosine-protein phosphatase non-receptor type substrate 1
SHP substrate 1
Brain Ig-like molecule with tyrosine-based activation motifs
Bit
CD172 antigen-like family member A
Inhibitory receptor SHPS-1
MyD-1 antigen
Signal-regulatory protein alpha-1
Sirp-alpha-1
mSIRP-alpha1
p84
CD172a

Background Information

SHPS-1 (also known as P84, BIT, and SIRP) is a neuronal membrane glycoprotein that promotes the attachment and neurite outgrowth of cultured murine cerebellar cells. Because of its heterophilic adhesive properties and its localization at synaptogenesis sites, SHPS-1 is suggested play a role in the regulation of synapse formation or maintenance. As a member of a phosphatase binding protein family, SHPS-1 shares identical homology with murine SHPS-1 cDNA. It is involved in receptor tyrosine kinase signaling that is found at synapses in the mammalian central nervous system and in non-neural tissues. It is reported that integrin-associated protein (IAP/CD47) is a predominant binding partner for P84. Upon phosphorylation of its cytoplasmic region, SHPS-1 binds to the protein-tyrosine phosphatase SHPS-2. Formation of the SHPS-1-SHP-2 complex is also implicated in the regulation of cellular migration. SHPS-1 and its ligand CD47 constitute an intercellular recognition system that contributes to the inhibition of cell migration via cell-cell contact. The ectodomain of SHPS-1 is shown to be excised from cells in a reaction that is likely mediated by a metalloproteinase. It is demonstrated that forced expression of a SHPS-1 mutant resistant to ectodomain shedding impaires cell migration, cell spreading, actin cytoskeleton reorganization, and paxillin- and FAK- tyrosine phosphorylation.

Product Information
Format	Purified
Control	Mouse brain tissue lysate
Presentation	Purified rat monoclonal IgG1k in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Purified

Control

Mouse brain tissue lysate

Presentation

Purified rat monoclonal IgG1k in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	Anti-SHPS-1 Antibody, clone P84 detects level of SHPS-1 & has been published & validated for use in WB, IC, IH.
Key Applications	Western Blotting Immunocytochemistry Immunohistochemistry Affects Function
Application Notes	Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in mouse astrocytes (Chuang, W., and Lagenaur, C.F., (1990). Developmental Biology. 137(2):219-232). Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in adult mouse retina (Jiang, P., Lagenaur, C.F., and Narayanan, V. (1999). J Biol Chem. 274(2):559-562). Neurite Outgrowth Inhibition Analysis: A representative lot was used by an independent laboratory to inhibit neurite outgrowth in mouse cerebellum cells (Chuang, W., and Lagenaur, C.F., (1990). Developmental Biology. 137(2):219-232).

Applications

Application

Anti-SHPS-1 Antibody, clone P84 detects level of SHPS-1 & has been published & validated for use in WB, IC, IH.

Key Applications

Western Blotting
Immunocytochemistry
Immunohistochemistry
Affects Function

Application Notes

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in mouse astrocytes (Chuang, W., and Lagenaur, C.F., (1990). Developmental Biology. 137(2):219-232).
Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in adult mouse retina (Jiang, P., Lagenaur, C.F., and Narayanan, V. (1999). J Biol Chem. 274(2):559-562).
Neurite Outgrowth Inhibition Analysis: A representative lot was used by an independent laboratory to inhibit neurite outgrowth in mouse cerebellum cells (Chuang, W., and Lagenaur, C.F., (1990). Developmental Biology. 137(2):219-232).

Biological Information
Immunogen	Linear peptide corresponding to mouse SHPS-1.
Clone	P84
Host	Rat
Isotype	IgG1κ
Species Reactivity	Mouse
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_001171117
Gene Symbol	Sirpa Bit Myd1 Ptpns1 Shps1 Sirp
Purification Method	Protein G Purified
UniProt Number	P97797
UniProt Summary	FUNCTION: Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. SUBUNIT STRUCTURE: Binds PTPN11 when tyrosine-phosphorylated, except in macrophages, where it primarily binds PTPN6. Binds GRB2 vitro. Binds FGR. Binds JAK2 irrespective of its phosphorylation status and forms a stable complex. Binds SCAP1 and/or SCAP2. The resulting complex recruits FYB. Binds PTK2B. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Highly expressed in cerebral cortex, brain, spinal cord, cerebellum and spleen, and at much lower levels in kidney, thymus, heart, lung and liver. Within the cerebellum, highly expressed throughout the molecular layer, and in synaptic glomeruli in the granule cell layer. Detected in neurons of the hippocampus and dentate gyrus, and in olfactory bulb. Not detected in Purkinje cells. Highly expressed in the plexiform layers, optic fiber layer and the outer segments of the photoreceptor layer in the retina. Highly expressed in macrophages. Isoform 3 is detected at very low levels in all tissues tested.
Molecular Weight	~100 kDa observed. The calculated molecular weight of this protein is 53 kDa, but can be observed at ~100-120 kDa due to high glycosolation (Comu, S., et al. (1997). J Neurosci. 17(22):8702-8710).

Biological Information

Immunogen

Linear peptide corresponding to mouse SHPS-1.

Clone

P84

Host

Rat

Isotype

IgG1κ

Species Reactivity

Mouse

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_001171117

Gene Symbol

Sirpa
Bit
Myd1
Ptpns1
Shps1
Sirp

Purification Method

Protein G Purified

UniProt Number

P97797

UniProt Summary

FUNCTION: Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.

SUBUNIT STRUCTURE: Binds PTPN11 when tyrosine-phosphorylated, except in macrophages, where it primarily binds PTPN6. Binds GRB2 vitro. Binds FGR. Binds JAK2 irrespective of its phosphorylation status and forms a stable complex. Binds SCAP1 and/or SCAP2. The resulting complex recruits FYB. Binds PTK2B.

SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.

TISSUE SPECIFICITY: Highly expressed in cerebral cortex, brain, spinal cord, cerebellum and spleen, and at much lower levels in kidney, thymus, heart, lung and liver. Within the cerebellum, highly expressed throughout the molecular layer, and in synaptic glomeruli in the granule cell layer. Detected in neurons of the hippocampus and dentate gyrus, and in olfactory bulb. Not detected in Purkinje cells. Highly expressed in the plexiform layers, optic fiber layer and the outer segments of the photoreceptor layer in the retina. Highly expressed in macrophages. Isoform 3 is detected at very low levels in all tissues tested.

Molecular Weight

~100 kDa observed.
The calculated molecular weight of this protein is 53 kDa, but can be observed at ~100-120 kDa due to high glycosolation (Comu, S., et al. (1997). J Neurosci. 17(22):8702-8710).

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in mouse brain tissue lysate. Western Blot Analysis: A 1:1,000 dilution of this antibody detected SPHS-1 in 10 µg of mouse brain tissue lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected SPHS-1 in 10 µg of mouse brain tissue lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-SHPS-1, clone P84 (rat monoclonal)

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