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Anti-MDM2, clone 4B2C1.11 (mouse monoclonal)

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货号：MABE331

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M WB, IP, ICC M Purified Monoclonal Antibody
Description
Catalogue Number MABE331
Description Anti-MDM2 Antibody, clone 4B2C1.11
Alternate Names
  • E3 ubiquitin-protein ligase Mdm2
  • Double minute 2 protein
  • Hdm2
  • Oncoprotein Mdm2
  • p53-binding protein Mdm2
Background Information MDM2 is over-expressed in many tumors. Its principal function is the ubiquitination and degradation of p53 tumour suppressor protein. This monoclonal antibody also recognizes a peptide epitope around Thr-216 of murine MDM2 when Thr-216 is unphosphorylated. SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.
References
Product Information
Format Purified
Control
  • Mouse brain tissue lysate
Presentation Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application Use Anti-MDM2 Antibody, clone 4B2C1.11 (mouse monoclonal antibody) validated in WB, IP, ICC to detect MDM2 also known as E3 ubiquitin-protein ligase Mdm2, Double minute 2 protein, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunocytochemistry
Application Notes Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated MDM2 in SV5-tagged, fusion p53-EGFP transfected H1299 cell lysates (Hjerpe, R., et al. (2010). 42(5):725-735.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected MDM2 in SV5-tagged, fusion p53-EGFP transfected H1299 cells (Hjerpe, R., et al. (2010). 42(5):725-735.).
Biological Information
Immunogen Recombinant protein corresponding to human MDM2.
Clone 4B2C1.11
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Isotype IgG1κ
Species Reactivity
  • Human
  • Mouse
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • MDM2
Purification Method Protein G Purified
UniProt Number
Molecular Weight ~55 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 0.25 µg/mL of this antibody detected MDM2 in 10 µg of mouse brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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