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Anti-FGF-2/Bfgf (mouse monoclonal)

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货号：MABS72

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
B, H NEUT M Purified Monoclonal Antibody
Description
Catalogue Number MABS72
Description Anti-FGF-2/bFGF Antibody
Alternate Names
  • BFGF
  • HBGF-2
Background Information FGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
References
Product Information
Format Purified
Control
  • FBHE cells
Presentation Purified mouse monoclonal IgG1κ in PBS without preservative.
Applications
Application Anti-FGF-2/bFGF Antibody is an antibody against FGF-2/bFGF for use in NEUT.
Key Applications
  • Neutralizing
Biological Information
Immunogen Human recombinant FGF-2/basic FGF
Clone 17F4.1
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Isotype IgG1κ
Species Reactivity
  • Bovine
  • Human
Species Reactivity Note Demonstrated to react with Bos Taurus (domestic cattle).
Predicted to react with human based on immunogen design.
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
Gene Symbol
  • FGF2
  • FGFB
Purification Method Protein G Purified
UniProt Number
UniProt Summary FUNCTION: The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.

SUBUNIT STRUCTURE: Monomer. Interacts with CSPG4 and FGFBP1. Found in a complex with FGFBP1, FGF1 and FGF2.

TISSUE SPECIFICITY: Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
Molecular Weight 31 kDa calculated.
Other isoforms exist at 17 kDa, 21 kDa, and 22 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Neutralization Assay in FBHE cells.

Neutralization Assay Analysis: 2.5 µg/mL of this antibody blocked 5 ng of FGF-2/bFGF in FBHE cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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