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Anti-N-Myc, clone NCM II 100 (mouse monoclonal)

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货号：MABE333

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB, IP, ICC M Purified Monoclonal Antibody
Description
Catalogue Number MABE333
Description Anti-N-Myc Antibody, clone NCM II 100
Alternate Names
  • N-myc proto-oncogene protein
  • Class E basic helix-loop-helix protein 37
  • bHLHe37
Background Information The v-myc oncogene, initially identified in the MC29 avian retrovirus, causes myelocytomas, carcinomas, sarcomas and lymphomas, and belongs to a family of oncogenes conserved throughout evolution. In humans the family consists of five genes: c-myc, N-myc, R-myc, L-myc and B-myc. Amplification of the N-myc gene has been found in human neuroblastomas and cell lines. The extent of N-myc amplification correlates well with the stage of neuroblastoma disease. Immunological studies have shown that the human N-myc gene gives rise to at least two nuclear phosphoproteins that exhibit relatively short (30 min) half lives in vivo and exhibit DNA binding properties in vitro.
References
Product Information
Format Purified
Control
  • IMR-32 cell lysate
Presentation Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application Use Anti-N-Myc Antibody, clone NCM II 100 (mouse monoclonal antibody) validated in WB, IP, ICC to detect N-Myc also known as N-myc proto-oncogene protein, Class E basic helix-loop-helix protein 37, bHLHe37.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunocytochemistry
Application Notes Immunofluorescence Analysis: A representative lot from an independent laboratory detected N-Myc in IMR5 cells (Ikegaki, N., et al. (1986). 83(16):5929-5933.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated N-Myc in IP (Brondyk, W. H., et al. (1991). 6(7):1269-1276.).
Biological Information
Immunogen Recombinant protein corresponding to human N-Myc.
Clone NCM II 100
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Isotype IgG1κ
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • MYCN
  • BHLHE37
  • NMYC
Purification Method Protein G Purified
UniProt Number
Molecular Weight ~55 kDa observed. Uniprot describes a molecular weight at ~50 kDa. Uniprot states that this protein may be phosphorylated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in IMR-32 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected N-Myc in 10 µg of IMR-32 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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