Anti-TSPAN8, clone CO-029 (1116NS-29) (mouse monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	IH(P), FC, WB, ABA	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

IH(P), FC, WB, ABA

Purified

Monoclonal Antibody

Description
Catalogue Number	MABC1161
Description	Anti-TSPAN8 Antibody, clone CO-029 (1116NS-29)
Alternate Names	Tetraspanin-8 Transmembrane 4 superfamily member 3 Tspan-8 Tumor-associated antigen CO-029
Background Information	Tetraspanin-8 (UniProt P19075; also known as Transmembrane 4 superfamily member 3, Tspan-8, Tumor-associated antigen CO-029) is encoded by the TSPAN8 (also known as TM4SF3) gene (Gene ID 7103) in human. CO-029 is a member of the transmembrane 4 or tetraspanin family of cell-surface 4-transmembrane glycoproteins involved in the regulation of cell activation, growth, and motility. Tetraspanin-8 (CO-029 antigen) was originally characterized as a tumor-associated antigen expressed in gastric, colorectal, and pancreatic cancer cells and exert tumor progression-promoting activity. In metastatic pancreatic and colorectal carcinoma cell lines, PKC activation enhances the colocalization of Tetraspanin-8 and tetraspanin CD151 with integrin α6β4 and subsequent internalization of this integrin-tetraspanin complex, resultinig in decreased cell-matrix adhesion and increased cell migration. Tetraspanin-8 knockdown in HT29 colon cancer cells is reported to significantly reduce cell migratory ability as a result of an upregulation of both integrin-dependent cell-matrix adhesion and calcium-dependent cell-cell adhesion. Human tetraspanin-8 is a four-transmembrane (10-33, 58-72, 84-109, 206-230) protein with both its N- and C-terminal ends (a.a. 1-9 and 231-237) exposed at the cytoplasmic side.

Description

Catalogue Number

MABC1161

Description

Anti-TSPAN8 Antibody, clone CO-029 (1116NS-29)

Alternate Names

Tetraspanin-8
Transmembrane 4 superfamily member 3
Tspan-8
Tumor-associated antigen CO-029

Background Information

Tetraspanin-8 (UniProt P19075; also known as Transmembrane 4 superfamily member 3, Tspan-8, Tumor-associated antigen CO-029) is encoded by the TSPAN8 (also known as TM4SF3) gene (Gene ID 7103) in human. CO-029 is a member of the transmembrane 4 or tetraspanin family of cell-surface 4-transmembrane glycoproteins involved in the regulation of cell activation, growth, and motility. Tetraspanin-8 (CO-029 antigen) was originally characterized as a tumor-associated antigen expressed in gastric, colorectal, and pancreatic cancer cells and exert tumor progression-promoting activity. In metastatic pancreatic and colorectal carcinoma cell lines, PKC activation enhances the colocalization of Tetraspanin-8 and tetraspanin CD151 with integrin α6β4 and subsequent internalization of this integrin-tetraspanin complex, resultinig in decreased cell-matrix adhesion and increased cell migration. Tetraspanin-8 knockdown in HT29 colon cancer cells is reported to significantly reduce cell migratory ability as a result of an upregulation of both integrin-dependent cell-matrix adhesion and calcium-dependent cell-cell adhesion. Human tetraspanin-8 is a four-transmembrane (10-33, 58-72, 84-109, 206-230) protein with both its N- and C-terminal ends (a.a. 1-9 and 231-237) exposed at the cytoplasmic side.

Product Information
Format	Purified
Presentation	Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Purified

Presentation

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	This Anti-TSPAN8 Antibody, clone CO-029 (1116NS-29) is validated for use in Immunohistochemistry (Paraffin), Flow Cytometry, Western Blotting, Affinity Binding Assay for the detection of TSPAN8.
Key Applications	Immunohistochemistry (Paraffin) Flow Cytometry Western Blotting Affinity Binding Assay
Application Notes	Flow Cytometry Analysis: 0.1 µg from a representative lot detected TSPAN8 in Caco-2 human epithelial colorectal adenocarcinoma cells. Affinity Binding Assay: A representative lot detected surface CO-029 antigen (TSPAN8) expression among a panel of human tumor cell lines by radioimmune assay (RIA) and mixed-hemadsorption assay (MHA) (Szala, S., et al. (1990). Proc. Natl. Acad. Sci. U. S. A. 87(17):6833-6837). Flow Cytometry Analysis: A representative lot detected surface CO-029 antigen (TSPAN8) immunoreactivity predominantly among tumor cells in pancreatic adenocarcinoma tissues-derived cell cultures (Gesierich, S., et al. (2005). Clin. Cancer Res. 11(8):2840-2852). Immunocytochemistry Analysis: A representative lot detected CO-029 antigen (TSPAN8) immunoreactivity colocalized with those of integrins alpha6 and beta4 by dual fluorescent immunocytochemistry staining of 8.18 human pancreatic cancer cells (Gesierich, S., et al. (2005). Clin. Cancer Res. 11(8):2840-2852). Immunocytochemistry Analysis: A representative lot detected CO-029 antigen (TSPAN8) immunoreactivity colocalized with that of integrin beta4 by dual fluorescent immunocytochemistry staining of human pancreatic cancer Capan1 cells, as well as human colon cancer HT29 and WIDR cells (Gesierich, S., et al. (2005). Clin. Cancer Res. 11(8):2840-2852). Immunohistochemistry Analysis: A representative lot detected an upregulated CO-029 antigen (TSPAN8) immunoreactivity in frozen pancreatic adenocarcinoma tissue sections than normal pancreatic tissue sections (Gesierich, S., et al. (2005). Clin. Cancer Res. 11(8):2840-2852). Western Blotting Analysis: A representative lot detected CO-029 antigen (TSPAN8) in integrin alpha3 immunoprecipitates from 8.18 human pancreatic cancer cell lysates (Gesierich, S., et al. (2005). Clin. Cancer Res. 11(8):2840-2852) Western Blotting Analysis: A representative lot detected the endogenous CO-029 antigen (TSPAN8) in SW948 human colon cancer cell lysate as well as the exogenously expressed CO-029 antigen in transfected COS cells (Szala, S., et al. (1990). Proc. Natl. Acad. Sci. U. S. A. 87(17):6833-6837).

Applications

Application

This Anti-TSPAN8 Antibody, clone CO-029 (1116NS-29) is validated for use in Immunohistochemistry (Paraffin), Flow Cytometry, Western Blotting, Affinity Binding Assay for the detection of TSPAN8.

Key Applications

Immunohistochemistry (Paraffin)
Flow Cytometry
Western Blotting
Affinity Binding Assay

Application Notes

Flow Cytometry Analysis: 0.1 µg from a representative lot detected TSPAN8 in Caco-2 human epithelial colorectal adenocarcinoma cells.
Affinity Binding Assay: A representative lot detected surface CO-029 antigen (TSPAN8) expression among a panel of human tumor cell lines by radioimmune assay (RIA) and mixed-hemadsorption assay (MHA) (Szala, S., et al. (1990). Proc. Natl. Acad. Sci. U. S. A. 87(17):6833-6837).
Flow Cytometry Analysis: A representative lot detected surface CO-029 antigen (TSPAN8) immunoreactivity predominantly among tumor cells in pancreatic adenocarcinoma tissues-derived cell cultures (Gesierich, S., et al. (2005). Clin. Cancer Res. 11(8):2840-2852).
Immunocytochemistry Analysis: A representative lot detected CO-029 antigen (TSPAN8) immunoreactivity colocalized with those of integrins alpha6 and beta4 by dual fluorescent immunocytochemistry staining of 8.18 human pancreatic cancer cells (Gesierich, S., et al. (2005). Clin. Cancer Res. 11(8):2840-2852).
Immunocytochemistry Analysis: A representative lot detected CO-029 antigen (TSPAN8) immunoreactivity colocalized with that of integrin beta4 by dual fluorescent immunocytochemistry staining of human pancreatic cancer Capan1 cells, as well as human colon cancer HT29 and WIDR cells (Gesierich, S., et al. (2005). Clin. Cancer Res. 11(8):2840-2852).
Immunohistochemistry Analysis: A representative lot detected an upregulated CO-029 antigen (TSPAN8) immunoreactivity in frozen pancreatic adenocarcinoma tissue sections than normal pancreatic tissue sections (Gesierich, S., et al. (2005). Clin. Cancer Res. 11(8):2840-2852).
Western Blotting Analysis: A representative lot detected CO-029 antigen (TSPAN8) in integrin alpha3 immunoprecipitates from 8.18 human pancreatic cancer cell lysates (Gesierich, S., et al. (2005). Clin. Cancer Res. 11(8):2840-2852)
Western Blotting Analysis: A representative lot detected the endogenous CO-029 antigen (TSPAN8) in SW948 human colon cancer cell lysate as well as the exogenously expressed CO-029 antigen in transfected COS cells (Szala, S., et al. (1990). Proc. Natl. Acad. Sci. U. S. A. 87(17):6833-6837).

Biological Information
Immunogen	SW1116 human colorectal carcinoma cells.
Epitope	Extracellular domain.
Clone	CO-029
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Specificity	Clone CO-029 (originally 1116NS-29) detected the glycosylated target bands in CO-029 antigen (TSPAN8) cDNA-transfected, but not untransfected COS cells (Szala, S., et al. (1990). Proc. Natl. Acad. Sci. U. S. A. 87(17):6833-6837).
Isotype	IgG1κ
Species Reactivity	Human
Species Reactivity Note	Human.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_004607
Gene Symbol	TSPAN8 TM4SF3
Purification Method	Protein G purified.
UniProt Number	P19075
Molecular Weight	26.04 kDa calculated. ~27-34 kDa reported (Szala, S., et al. (1990). Proc. Natl. Acad. Sci. U. S. A. 87(17):6833-6837) due to glycosylation.

Biological Information

Immunogen

SW1116 human colorectal carcinoma cells.

Epitope

Extracellular domain.

Clone

CO-029

Concentration

Please refer to lot specific datasheet.

Host

Mouse

Specificity

Clone CO-029 (originally 1116NS-29) detected the glycosylated target bands in CO-029 antigen (TSPAN8) cDNA-transfected, but not untransfected COS cells (Szala, S., et al. (1990). Proc. Natl. Acad. Sci. U. S. A. 87(17):6833-6837).

Isotype

IgG1κ

Species Reactivity

Human

Species Reactivity Note

Human.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_004607

Gene Symbol

TSPAN8
TM4SF3

Purification Method

Protein G purified.

UniProt Number

P19075

Molecular Weight

26.04 kDa calculated. ~27-34 kDa reported (Szala, S., et al. (1990). Proc. Natl. Acad. Sci. U. S. A. 87(17):6833-6837) due to glycosylation.

Product Usage Statements
Quality Assurance	Evaluated by Immunohistochemistry in human colon cancer tissue. Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected TSPAN8 in human colon cancer tissue section.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Immunohistochemistry in human colon cancer tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected TSPAN8 in human colon cancer tissue section.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 μg

Packaging Information

Material Size

100 μg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-TSPAN8, clone CO-029 (1116NS-29) (mouse monoclonal)

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