Anti-hnRNP Q, clone 7A11.2 (mouse monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	WB, ICC	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

WB, ICC

Purified

Monoclonal Antibody

Description
Catalogue Number	MAB11004
Description	Anti-hnRNP Q Antibody, clone 7A11.2
Alternate Names	heterogeneous nuclear ribonucleoprotein Q-like Synaptotagmin-binding, cytoplasmic RNA-interacting protein Glycine- and tyrosine-rich RNA-binding protein GRY-RBP NS1-associated protein 1
Background Information	Heterogeneous nuclear ribonucleoprotein Q, commonly refered to as hnRNP Q, can be found as three different isoforms derived from alternative splicing of a single gene. This RNA binding protein is implicated in control of mRNA precursor splicing for the survival of motor neurons (SMN) protein. Loss-of-function mutations of SMN are linked to spinal muscular atrophy (SMA) a common neurodegenerative disease. It has been shown that hnRNP Q proteins interact with SMN, and this is interacton is required for efficient pre-mRNA splicing in vitro. Current data suggests that hnRNP Q is a splicing modulator of SMN.

Description

Catalogue Number

MAB11004

Description

Anti-hnRNP Q Antibody, clone 7A11.2

Alternate Names

heterogeneous nuclear ribonucleoprotein Q-like
Synaptotagmin-binding, cytoplasmic RNA-interacting protein
Glycine- and tyrosine-rich RNA-binding protein
GRY-RBP
NS1-associated protein 1

Background Information

Heterogeneous nuclear ribonucleoprotein Q, commonly refered to as hnRNP Q, can be found as three different isoforms derived from alternative splicing of a single gene. This RNA binding protein is implicated in control of mRNA precursor splicing for the survival of motor neurons (SMN) protein. Loss-of-function mutations of SMN are linked to spinal muscular atrophy (SMA) a common neurodegenerative disease. It has been shown that hnRNP Q proteins interact with SMN, and this is interacton is required for efficient pre-mRNA splicing in vitro. Current data suggests that hnRNP Q is a splicing modulator of SMN.

Product Information
Format	Purified
Control	NIH/3T3 cell lysate
Presentation	Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Purified

Control

NIH/3T3 cell lysate

Presentation

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	Anti-hnRNP Q Antibody, clone 7A11.2 is a Mouse Monoclonal Antibody for detection of hnRNP Q also known as heterogeneous nuclear ribonucleoprotein Q-like & has been validated in WB, ICC.
Key Applications	Western Blotting Immunocytochemistry
Application Notes	Western Blot Analysis: 1 µg/mL from a representative lot detected hnRNP Q on 10 µg of PC-12 and HEK293 cell lysate. Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected hnRNP Q in NIH/3T3, A431, and HeLa cells.

Applications

Application

Anti-hnRNP Q Antibody, clone 7A11.2 is a Mouse Monoclonal Antibody for detection of hnRNP Q also known as heterogeneous nuclear ribonucleoprotein Q-like & has been validated in WB, ICC.

Key Applications

Western Blotting
Immunocytochemistry

Application Notes

Western Blot Analysis: 1 µg/mL from a representative lot detected hnRNP Q on 10 µg of PC-12 and HEK293 cell lysate.

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected hnRNP Q in NIH/3T3, A431, and HeLa cells.

Biological Information
Immunogen	Full length recombinant protein corresponding to human hnRNPQ.
Clone	7A11.2
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Isotype	IgG1κ
Species Reactivity	Human Mouse Rat
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_001153145
Gene Symbol	hnRNP-Q hnRNP Q SYNCRIP HNRPQ NSAP1
Purification Method	Protein G
UniProt Number	O60506
UniProt Summary	FUNCTION: Heterogenous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Component of the CRD-mediated complex that promotes MYC mRNA stability. Isoform 1, isoform 2 and isoform 3 are associated in vitro with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences. Isoform 1 is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. Isoform 3 may be involved in cytoplasmic vesicle-based mRNA transport through interaction with synaptotagmins. SUBUNIT STRUCTURE: Isoform 1 is a component of the APOB mRNA editosome complex and interacts with APOBEC1 and A1CF (APOBEC1 complementation factor). Part of a complex associated with the FOS mCRD domain and consisting of PABPC1, PAIP1, CSDE1/UNR, HNRPD and SYNCRIP. Isoform 3 interacts with HNRPR. Interacts with POLR2A hyperphosphorylated C-terminal domain. Interacts with minute virus of mice (MVM) NS1 protein. Isoform 1, isoform 2 and isoform 3 interact with SMN. Isoform 3 interacts through its C-terminal domain with SYT7, SYT8 and SYT9 (By similarity). The non-phosphorylated and phosphorylated forms are colocalized with PAIP1 in polysomes (By similarity). Identified in a histone pre-mRNA complex, at least composed of ERI1, LSM11, SLBP, SNRPB, SYNCRIP and YBX1 (By similarity). Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8. Component of the coding region determinant (CRD)-mediated complex, composed of DHX9, HNRNPU, IGF2BP1, SYNCRIP and YBX1. Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. SUBCELLULAR LOCATION: Cytoplasm. Microsome (By similarity). Endoplasmic reticulum (By similarity). Nucleus (By similarity). Note: The tyrosine phosphorylated form bound to RNA is found in microsomes (By similarity). Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Isoform 1: Nucleus › nucleoplasm (By similarity). Note: Expressed predominantly in the nucleoplasm (By similarity). Isoform 2: Nucleus › nucleoplasm (By similarity). Note: Expressed predominantly in the nucleoplasm (By similarity). Isoform 3: Nucleus › nucleoplasm (By similarity). Note: Expressed predominantly in the nucleoplasm (By similarity). TISSUE SPECIFICITY: Ubiquitously expressed. Detected in heart, brain, pancreas, placenta, spleen, lung, liver, skeletal muscle, kidney, thymus, prostate, uterus, small intestine, colon, peripheral blood and testis. Domain The domain containing eight Arg-Gly-Gly repeats may be involved in RNA-binding and protein-protein interactions. PTM: Phosphorylated on tyrosine. The membrane-bound form found in microsomes is phosphorylated in vitro by insulin receptor tyrosine kinase (INSR). Phosphorylation is inhibited upon binding to RNA, whereas the cytoplasmic form is poorly phosphorylated By similarity. Phosphorylated upon DNA damage, probably by ATM or ATR. SEQUENCE SIMILARITIES: Contains 3 RRM (RNA recognition motif) domains. SEQUENCE CAUTION: The sequence AAH15575.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Potential poly-A sequence starting in position 413.
Molecular Weight	~59 observed. Mulitple isoforms may be observed in some lysates.

Biological Information

Immunogen

Full length recombinant protein corresponding to human hnRNPQ.

Clone

7A11.2

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Isotype

IgG1κ

Species Reactivity

Human
Mouse
Rat

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_001153145

Gene Symbol

hnRNP-Q
hnRNP Q
SYNCRIP
HNRPQ
NSAP1

Purification Method

Protein G

UniProt Number

O60506

UniProt Summary

FUNCTION: Heterogenous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Component of the CRD-mediated complex that promotes MYC mRNA stability. Isoform 1, isoform 2 and isoform 3 are associated in vitro with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences. Isoform 1 is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. Isoform 3 may be involved in cytoplasmic vesicle-based mRNA transport through interaction with synaptotagmins.

SUBUNIT STRUCTURE: Isoform 1 is a component of the APOB mRNA editosome complex and interacts with APOBEC1 and A1CF (APOBEC1 complementation factor). Part of a complex associated with the FOS mCRD domain and consisting of PABPC1, PAIP1, CSDE1/UNR, HNRPD and SYNCRIP. Isoform 3 interacts with HNRPR. Interacts with POLR2A hyperphosphorylated C-terminal domain. Interacts with minute virus of mice (MVM) NS1 protein. Isoform 1, isoform 2 and isoform 3 interact with SMN. Isoform 3 interacts through its C-terminal domain with SYT7, SYT8 and SYT9 (By similarity). The non-phosphorylated and phosphorylated forms are colocalized with PAIP1 in polysomes (By similarity). Identified in a histone pre-mRNA complex, at least composed of ERI1, LSM11, SLBP, SNRPB, SYNCRIP and YBX1 (By similarity). Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8. Component of the coding region determinant (CRD)-mediated complex, composed of DHX9, HNRNPU, IGF2BP1, SYNCRIP and YBX1. Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs.

SUBCELLULAR LOCATION: Cytoplasm. Microsome (By similarity). Endoplasmic reticulum (By similarity). Nucleus (By similarity). Note: The tyrosine phosphorylated form bound to RNA is found in microsomes (By similarity). Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Isoform 1: Nucleus › nucleoplasm (By similarity). Note: Expressed predominantly in the nucleoplasm (By similarity).

Isoform 2: Nucleus › nucleoplasm (By similarity). Note: Expressed predominantly in the nucleoplasm (By similarity).

Isoform 3: Nucleus › nucleoplasm (By similarity). Note: Expressed predominantly in the nucleoplasm (By similarity).

TISSUE SPECIFICITY: Ubiquitously expressed. Detected in heart, brain, pancreas, placenta, spleen, lung, liver, skeletal muscle, kidney, thymus, prostate, uterus, small intestine, colon, peripheral blood and testis.

Domain The domain containing eight Arg-Gly-Gly repeats may be involved in RNA-binding and protein-protein interactions.

PTM: Phosphorylated on tyrosine. The membrane-bound form found in microsomes is phosphorylated in vitro by insulin receptor tyrosine kinase (INSR). Phosphorylation is inhibited upon binding to RNA, whereas the cytoplasmic form is poorly phosphorylated By similarity. Phosphorylated upon DNA damage, probably by ATM or ATR.

SEQUENCE SIMILARITIES: Contains 3 RRM (RNA recognition motif) domains.

SEQUENCE CAUTION: The sequence AAH15575.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Potential poly-A sequence starting in position 413.

Molecular Weight

~59 observed. Mulitple isoforms may be observed in some lysates.

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in NIH/3T3 cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected hnRNP Q on 10 µg of NIH/3T3 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in NIH/3T3 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected hnRNP Q on 10 µg of NIH/3T3 cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-hnRNP Q, clone 7A11.2 (mouse monoclonal)

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