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Anti-phospho-Adducin (Ser662)

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货号：06-820

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
Ca, H, M, R WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number 06-820
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-phospho-Adducin (Ser662) Antibody
References
Product Information
Format Affinity Purified
Presentation 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide, and 30% glycerol. Liquid at -20°C.
Applications
Application This Anti-phospho-Adducin (Ser662) Antibody is validated for use in WB for the detection of phospho-Adducin (Ser662).
Key Applications
  • Western Blotting
Biological Information
Immunogen peptide (C-KKFRTPpSFLKKNK) corresponding to amino acids 656-668 of human gamma-Adducin
Host Rabbit
Specificity phosphorylated serine 662 of γ-Adducin, phosphorylated serine 724 of α-Adducin, phosphorylated serine 713 of β-Adducin
Isotype IgG
Species Reactivity
  • Canine
  • Human
  • Mouse
  • Rat
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced adducin gamma transcripts encoding different isoforms have been described. The functions of the different isoforms are not known.
Gene Symbol
  • ADD3
  • adducin-like
  • ADDL
  • Gamma-adducin
Modifications
  • Phosphorylation
Purification Method ImmunoAffinity Purified
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q9UEY8 # Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
SIZE: 706 amino acids; 79155 Da
SUBUNIT: Heterodimer of an alpha and a gamma subunit.
TISSUE SPECIFICITY: Heart only expresses isoform 1.
DOMAIN:SwissProt: Q9UEY8 Comprised of three regions: a N-terminal protease- resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
PTM: Sumoylated.
SIMILARITY: Belongs to the aldolase class II family. Adducin subfamily.
Molecular Weight 80kDa, 120kDa, 110kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance routinely evaluated by immunoblot on RIPA lysates from 3T3/NIH cells or on canine MDCK cells
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions 1 year at -20°C
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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