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Milli-Mark™ Anti-Nestin-PE clone 10C2

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货号：FCMAB313PE

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H FC M Phycoerythrin Monoclonal Antibody
Description
Catalogue Number FCMAB313PE
Trade Name
  • Milli-Mark
Description Milli-Mark® Anti-Nestin-PE Antibody, clone 10C2
Background Information Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma.
References
Product Information
Format Phycoerythrin
Control
  • U251 cells
Presentation Purified mouse monoclonal IgG1κ conjugated to PE in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Applications
Application Detect Nestin using this Milli-Mark Anti-Nestin-PE Antibody, clone 10C2 validated for use in FC.
Key Applications
  • Flow Cytometry
Biological Information
Immunogen Fusion protein
Epitope Human
Clone 10C2
Host Mouse
Specificity Antibody recognizes Human Nestin.
Isotype IgG1κ
Species Reactivity
  • Human
Species Reactivity Note Tested and passed on human. No reactivity with mouse or rat Nestin.
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.
Gene Symbol
  • NES
  • Nbla00170
Purification Method Protein A Purfied
UniProt Number
UniProt Summary FUNCTION: SwissProt: P48681 # May play a role in the trafficking and distribution of IF proteins and potentially other cellular factors to daughter cells during progenitor cell division (By similarity).

SIZE: 1621 amino acids; 177439 Da

SUBUNIT: Interacts with FHOD3 (By similarity).

TISSUE SPECIFICITY: CNS stem cells.

DEVELOPMENTAL STAGE: Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.

SIMILARITY: SwissProt: P48681 ## Belongs to the intermediate filament family.
Molecular Weight 177 kDa Calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by flow cytometry using U251 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Packaging Information
Material Size 100 tests
Transport Information
Supplemental Information
Specifications

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