[关闭]
您好！欢迎来到炼石商城   请登录  注册    兼职小伙伴  
我的购物车
0
Qiagen 凯杰 Biosharp Omega

首页抗体Millipore 密理博>

图片仅供参考，请以实物为准
收藏

Anti-Cytocrome P450 1A1, clone 17D7.1 (mouse monoclonal)

市场价：
0.00
促销价：
¥0.00

货号：MABS19

品牌：Millipore 密理博

配送至：

支付： 账期 货到付款

数量：

EA (预计3-5工作日到货)

亲，想了解价格请QQ咨询客服！

工作时间

周一至周五：9:00-18:00

咨询电话

0771-3293894

在线咨询

客服 郭恒 蔡玉坤 曾宪飞 技术咨询

浏览了该商品的用户最终购买

RNeasy Mini Kit (50) 50次
RNeasy Mini Kit (50) 50次
3720.00
Plasmid Mini Kit I(200) 200次
Plasmid Mini Kit I(200) 200...
576.00
10ul吸头，透明，袋装，可灭菌 1000支/袋，50袋/箱
10ul吸头，透明，袋装，可灭菌...
85.80

浏览了该商品的用户还浏览了

QIAfilter Plasmid Maxi Kit (10) -10
QIAfilter Plasmid Maxi Kit (...
3000.00
QIAamp DNA Blood Midi Kit (20) 20次
QIAamp DNA Blood Midi Kit (2...
2680.00
QIAamp UCP Pathogen Mini Kit (50)
QIAamp UCP Pathogen Mini Kit...
4112.00
Replacement Information

特卖

100% Performance Guaranteed

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB, IP, ICC Rb Purified Monoclonal Antibody
Description
Catalogue Number MABS199
Description Anti-Linear Ubiquitin, clone 1E3 Antibody
Alternate Names
  • Polyubiquitin-C
  • Ubiquitin
Background Information Ubiqutin (Ub) is initially produced as a 229-a.a. Polyubiquitin-B (UniProt P0CG47) precursor protein encoded by the UBB gene (Gene ID 7314) or a 685-a.a. Polyubiquitin-C precursor protein (UniProt P0CG48) encoded by the UBC gene (Gene ID 7316) in human. The C-termini of Polyubiquitin-B (Cys229) and Polyubiquitin-C (Val685) are post-translationally removed, followed by additional posttranslational cleavages to yield multiple copies of idential 76-amino acid ubiquitin (also known as high mobility group nonhistone protein HMG-20) molecules (three copies per Polyubiquitin-B and nine copiesf per Polyubiquitin-C). Ub is involved in one of the most common post-translational modifications (PTMs) of cellular proteins, where Ub is linked covalently via its carboxyl terminus (Gly76) to usually Lys residues in target proteins. A given target Lys residue can be linked to one single Ub molecule (monoubiquitylated) or to a chain of Ub molecules (polyubiquitylated). In a polyUb chain, Ub molecules can be linked through one of the seven Lys residues (K6, K11, K27, K29, K33, K48, and K63) or through the Ub N-terminus Met1 residue (which generates linear chains). An Ub chain with a single linkage type is referred to as homotypic, whereas an heterotypic chain contains mixed linkages within the same chain, which can lead to either branched/forked (as a result of one Ub linked to two or more Ubs) or non-branched Ub chains. Lys48-linked chains mark proteins for proteasomal degradation, while Lys63-linked chains regulate the activity/function of cellular proteins.
References
Product Information
Format Purified
Presentation Purified rabbit monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application This Anti-Ubiquitin antibody, clone 1E3 is validated for use in western blotting, IP & ICC for the detection of Linear Ubiquitin.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunocytochemistry
Application Notes Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected linear ubiquitin in Hoi-1L/Hoip transfected Hela cells.

Immunoprecipitatation Analysis: 10 µg from a representative lot immunoprecipitated linear ubiquitin from 500 µg of Hoi-1L/Hoip transfected HEK293 cells.

Western Blotting Analysis: A representative lot from an independent laboratory detected K64-linked diUbiquitin (Matsumoto, M. L., et al. (2012). J Mol Biol. 418(3-4):134-144.).
Biological Information
Immunogen Recombinant protein corresponding to all linear ubiquitin linkages.
Clone 1E3
Host Rabbit
Isotype IgGκ
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Gene Symbol
  • UBC
Purification Method Protein A Purfied
UniProt Number
Molecular Weight Multiple molecular weight bands observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blotting in linear polyubiquitin chains, K48 Ubiquitin 2-7, K63 Ubiquitin 2-7, and K11-only Ubn-ubiquitinylated HM substrate protein synthesized using K11-only mutant ubiquitin.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected linear ubiquitin chains, and not K48 Ubiquitin 2-7, K63 Ubiquitin 2-7, or K11-only Ubn-ubiquitinylated HM substrate protein synthesized using K11-only mutant ubiquitin.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

温馨提示:因厂家更改产品包装、产地或者更换随机附件等没有任何提前通知，且每位咨询者购买情况、提问时间等不同，为此以下回复仅对提问者3天内有效，其他网友仅供参考！若由此给您带来不便请多多谅解，谢谢！

我要咨询

咨询内容：
购物指南 免费注册 批量采购 购物流程 购物保障
配送方式 上门自提 第三方配送 炼石配送
支付方式 货到付款 账期付款
售后服务 售后政策 退款说明 售后流程 免责条款

服务热线

0771-3293894

关于我们|帮助中心|诚聘英才|炼石云实验室|联系我们|友情链接

Copyright 2014-2025 炼石云商网络科技（海南）有限公司版权所有 琼ICP备2023006525号-3